Specific primers for identifying Chiua virens and method for identifying Chiua virens by the specific primers

Abstract

The invention discloses specific primers for identifying Chiua virens and a method for identifying Chiua virens by the specific primers. The specific primers for identifying Chiua virens are CVf and CVr. A DNA sequence of CVf is CTTACCGAACGGAMGGGATAACACC and a DNA sequence of CVr is AGAAGCAAAGNACAGGAAAAGCATA. The method for identifying Chiua virens by the specific primers comprises the following steps of extracting a total DNA of a mixed bolete sample, carrying out specific primer PCR amplification, and carrying out agarose gel electrophoresis detection of amplification products to identify if the mixed bolete sample is mixed with toxic and unclean Chiua virens. The method for identifying Chiua virens by the specific primers does not need a PCR product sequencing process and can realize rapid and accurate identification, wherein generally, time from mixed bolete sample taking to identification result production is in a range of 2 to 3 hours.

Description

technical field [0001] The present invention relates to a kind of boletus viridis ( Chiua virens (W. F. Chiu) Yan C. Li & Zhu L. Yang) PCR identification method and its specific primers, which belong to the technical field of identifying boletus with unclear food poisoning by molecular biological methods. Background technique [0002] Yunnan's diverse three-dimensional climate and complex habitats have bred a wealth of wild mushroom resources. These resources play an important role in increasing the income of farmers in mountainous areas. In some remote areas, the income of wild mushrooms accounts for about 1 / 3-1 of the annual household income. / 2. Among wild fungus resources, boletus resources are the most widely distributed, most diverse, highly morphologically similar, relatively difficult to identify morphologically, and have the largest amount of resources. The fungal tissue of the boletus group is fleshy, prone to insects and decay, so in order to facilitate storage...

AI Technical Summary

Problems solved by technology

Accurate identification of boletus with unclear food toxicity from mixed commercial boletus requires a certain basis of large-scale fungal taxonomy, but consumers often do not have this knowledge. Therefore, on the one hand, it leads to poisoning The occurrence of incidents, on the other hand, connived at unscrupulous dealers

At present, the domestic research on Boletus viridis mainly focuses on morphological classification and systematic research, and there is no report on the rapid identification or detection of the bacteria using its specific primers

Images