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Boletus aereus mother strain preservation method, preservation culture medium and preparation method thereof

The technology of a mother seed culture medium and black bolete, which is applied in the field of mother seed preservation, can solve the problems of high cost, reduced activity of the mother seed, and high equipment requirements, so as to meet the needs of succession and propagation, realize stable preservation, and reduce impurities. The effect of bacterial infection

Pending Publication Date: 2021-10-29
JINGHONG HONGZHEN AGRI TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the preservation methods of the mother species of Boletus nigra mainly include inclined-plane low-temperature preservation, paraffin-sealing preservation, sterile water preservation, liquid nitrogen ultra-low temperature preservation, etc., and these preservation methods all have the following problems: ①Short storage period: inclined-plane preservation, 2- 4 months, stored in sterile water, 8-12 months; ②The activity of the parent species is reduced: paraffin sealing preservation and sterile water preservation methods, the survival rate of the preservation time exceeds 6 months is reduced to about 50%; ③High cost: liquid Nitrogen cryopreservation requires high equipment, complicated operation, and high loss; ④ The excellent traits of the strain change: the storage time exceeds 1 year, the mother species has weak resistance to impurities, poor cultivation traits, and serious yield reduction

Method used

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  • Boletus aereus mother strain preservation method, preservation culture medium and preparation method thereof
  • Boletus aereus mother strain preservation method, preservation culture medium and preparation method thereof
  • Boletus aereus mother strain preservation method, preservation culture medium and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] A culture medium for preserving boletus female species consists of the following components: 200g of fine wood chips, 100g of coarse wood chips, 50g of coffee husks, 100g of peat soil, 2g of potassium dihydrogen phosphate, 0.5g of magnesium sulfate, and 500ml of water.

[0047] The preparation method of the black boletus female species preservation medium: weigh 200g fine wood chips, 100g coarse wood chips, 50g coffee shells, 100g peat soil and stir them evenly, mix water and other medicines with the substrate, pH natural, divide Put it into a test tube with a specification of 150mm×15mm, the filling amount is half of the length of the test tube, and sterilize at 121°C for 30 minutes.

[0048] The black boletus female species preservation method of the present embodiment adopts the black boletus female species preservation medium to preserve the black boletus female species, comprising the following steps:

[0049] (1) Cut the mycelium into 0.3mm×0.3mm mycelium pieces, ...

Embodiment 2

[0056] In embodiment 2 and embodiment 3, the mother seed culture medium, mother seed cultivation and division, mother seed inoculation and cultivation, mother seed preservation, and mother seed viability detection are the same as in Example 1, and the difference is the proportion of the preservation medium , see Table 1 for details:

[0057] Table 1 Comparison of preservation effects of different preservation media

[0058]

[0059]

[0060] As can be seen from Table 1, under the same conditions of preservation, the growth rate of mycelium of embodiment 2 and embodiment 3 is fast, higher than that of embodiment 1, activated after two years of preservation, the time required for germination is short, and the survival rate is high. It shows that the preservation effect of mother species is better after adding aspartic acid. Amino acids can be converted into carbohydrates and fats. After adding a small amount of aspartic acid, the carbohydrates in the preservation medium c...

Embodiment 4

[0062] In embodiment 4 and embodiment 5, preservation culture medium, mother seed culture medium, mother seed cultivation and division, mother seed preservation, mother seed viability detection are the same as in Example 1, and the difference is the mother seed inoculation block: embodiment 4. The inoculation block is 5-10 days old and the tip hyphae; the inoculation block in Example 5 is 20-30 days old and the inner ring hyphae. See Table 2 for details:

[0063] Table 2 Comparison of preservation effects of different inoculation blocks

[0064]

[0065]

[0066] As can be seen from Table 2, under the same condition of the preservation medium, the growth rate of mycelium in Example 4 is significantly higher than that in Implementation 1 and Example 5, and it is activated after 2 years of preservation. The time required for germination in Example 4 is short, and the mother The species survival rate is high, significantly higher than that of Example 1 and Example 5, indic...

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Abstract

According to the technical scheme, the invention provides a boletus aereus mother strain preservation method, a preservation culture medium and a preparation method thereof, and the preservation culture medium comprises 50-200 g of coarse sawdust, 100-400 g of fine sawdust, 25-125 g of coffee shells, 25-150 g of turfy soil, 1-5 g of monopotassium phosphate, 0.5-3 g of magnesium phosphate and 300-700 ml of water. The preservation culture medium and the preparation method thereof are low in cost and simple to operate, and the preservation method of the boletus aereus mother strain by using the preservation culture medium is long-term, stable, simple and convenient.

Description

technical field [0001] The invention relates to the technical field of mother species preservation, in particular to a method for preserving the mother species of Boletus nigricans, a preservation medium and a preparation method thereof. Background technique [0002] Black boletus (Phlebopus portentosus) belongs to the family Boletaceae of the order Boletus and is one of the rare and delicious edible fungi. It is rich in various essential amino acids and mineral elements such as phosphorus, potassium and calcium. The scale of industrial cultivation of black boletus is growing day by day, and the importance of the mother species as the source of production is self-evident. [0003] At present, the preservation methods of the mother species of Boletus nigra mainly include inclined-plane low-temperature preservation, paraffin-sealing preservation, sterile water preservation, liquid nitrogen ultra-low temperature preservation, etc., and these preservation methods all have the fo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/04C12N1/14C12R1/645
CPCC12N1/04C12N1/14
Inventor 晏汐蓓纪开萍纪光燕陶玲刀明晶罗顺珍纪光玉玉应香
Owner JINGHONG HONGZHEN AGRI TECH CO LTD
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