Chinese chestnut wine and preparation method thereof Chinese chestnut fungus root preparations and preparation method thereof

A technology of chestnut fungi and preparations, applied in botany equipment and methods, biocides, animal repellents, etc., can solve the problems of long seedling breeding cycle, poor absorption capacity, weak healing ability, etc., to improve growth level, enhance Absorptive capacity and the effect of small wilting coefficient

Inactive Publication Date: 2007-11-28
秦岭
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, chestnut seedlings have poor ability to absorb fertilizer and water, and their ability to heal wounds is also weak. In produ...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 1. Selection of strains: Shanxi Rhizopus edulis and Boletus edulis isolated from the chestnut forest in Huairou Bohai Sea and the ectomycorrhizal fungus Rhizopus genus (Rhizopogon) symbiotic with chestnuts were selected.

[0028] 2. Strain culture:

[0029] 1) Activation of strains: a small amount of hyphae were picked from the strains Rhizogenus Shanxi and Boletus cerevisiae cultured on the inclined plane with a sterilized inoculation needle and connected to the PDA plate medium for activation. Activation conditions: 25°C, dark culture for 3 days, when the hyphae grow to 1 / 3 of the diameter of the petri dish. Wherein the PDA medium is: 200g of potatoes, 20g of glucose, 18g of agar, and 1000ml of distilled water.

[0030] 2) Liquid fermentation culture: pick a small amount of activated hyphae from the plate medium with a sterilized inoculation needle, and inoculate them into a liquid culture bottle, and the medium is PDA or ODA. Culture conditions: temperature 25°C, p...

Embodiment 2

[0035] 1. Selection of strains: choose Rhizopus edulis and Boletus edulis isolated from the chestnut forest in Huairou Bohai Sea and the ectomycorrhizal fungus Rhizopus genus (Rhizopogon) symbiotic with chestnuts.

[0036] 2. Strain culture:

[0037] 1) Activation of strains: use a sterilized inoculation needle to pick a small amount of mycelia from the strains of Rhizobia rubrum and Boletus cerevisiae cultured on a slant and connect them to the PDA plate medium for activation. Activation conditions: 25°C, dark culture for 5 days, when the hyphae grow to 1 / 2 of the diameter of the petri dish. Wherein the PDA medium is: 150g of potatoes, 25g of glucose, 20g of agar, and 1000ml of distilled water.

[0038] 2) Liquid fermentation culture: pick a small amount of activated hyphae from the plate medium with a sterilized inoculation needle, and inoculate them into a liquid culture bottle, and the medium is PDA or ODA. Culture conditions: temperature 25°C, pH about 5, shaking cultur...

Embodiment 3

[0043] 1. Selection of strains: select the ectomycorrhizal fungus Rhizopogon and Boletus edulis isolated from chestnut forests in Huairou Bohai Sea and symbiotic with chestnuts.

[0044] 2. Strain culture:

[0045] 1) Activation of strains: use a sterilized inoculation needle to pick a small amount of hyphae from the strains of Rhizobia nigeri and Boletus cerevisiae cultured on an inclined plane and connect them to the PDA plate medium for activation. Activation conditions: 25°C, dark culture for 4 days, when the hyphae grow to 1 / 3 of the diameter of the petri dish. Wherein the PDA medium is: 250g of potatoes, 15g of glucose, 15g of agar, and 1000ml of distilled water.

[0046]2) Liquid fermentation culture: pick a small amount of activated hyphae from the plate medium with a sterilized inoculation needle, and inoculate them into a liquid culture bottle, and the medium is PDA or ODA. Training conditions:

[0047] The temperature is 25°C, the pH is about 5, and the shaking c...

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Abstract

The present invention discloses one kind of Chinese chestnut mycorrhiza preparation obtained with Rhizopogon and Boletus edulis and through simultaneous culturing. The Chinese chestnut mycorrhiza preparation can raise the fertilizer and water absorbing ability of Chinese chestnut root system, expand the absorption area, decompose soil nutrients, strengthen drought fighting capacity, and secrete hormone and organic acid to enhance physiological activity of root system.

Description

technical field [0001] The invention relates to a chestnut mycorrhizal preparation and a preparation method thereof. Background technique [0002] Chestnut is one of the characteristic fruit trees in our country, and it is a traditional product that earns foreign exchange through export. Chestnut grows in mountainous areas with poor soil. Drought and nutritional deficiencies are the main factors limiting chestnut production. [0003] For example, in the Yanshan Mountains and Taihang Mountains, planting chestnuts is an effective way to develop barren hills and maintain water and soil. However, the natural conditions in these mountainous areas are relatively poor: the soil layer is dry and barren. However, chestnut seedlings have poor ability to absorb fertilizer and water, and their ability to callus is also weak. In production, they are generally planted with large seedlings that are more than 2 years old, and the seedling period is long and the cost is high. Contents o...

Claims

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Application Information

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IPC IPC(8): A01N63/00A01P21/00
Inventor 秦岭
Owner 秦岭
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