Method for identifying and detecting boletus bainiugan dentinger or components of boletus bainiugan dentinger

A detection method, the technology of white boletus, is applied in the field of fluorescent PCR identification and detection, which can solve the problems that the identification cannot meet very high standards, and achieve the effects of improving technical influence, accurate identification results, and short detection time

Pending Publication Date: 2020-01-31
昆明海关技术中心
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  • Abstract
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  • Claims
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AI Technical Summary

Problems solved by technology

Although mass spectrometry can be used to identify species, its identification of a certain bacterial species cannot reach a very high standard. Therefore, more accurate and targeted identification methods should be used when appropriate and necessary. Species identification of wild fungi has become a necessary solution

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  • Method for identifying and detecting boletus bainiugan dentinger or components of boletus bainiugan dentinger

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Embodiment Construction

[0020] 1. An identification and detection method of boletus or its components, the detection method is a fluorescent PCR detection method. The white boletus that the present invention refers to is Boletus bainiugan Dentinger.

[0021] Further, the primers used in the detection method of the present invention are: upstream primer (F) 5'-GGATCATTATCGAGTTAGAC-3'; downstream primer (R) 5'-TGGACATGCAATAGAGTA-3'.

[0022] Further, the probe used in the detection method of the present invention is: probe (P)(HEX)TTCCTCGGACTCTCCTTCCTAGT (Eclipse).

[0023] Further, the reaction system of the detection method of the present invention is:

[0024] Reaction system components Sample volume Final concentration 2×premix Ex Taq(Probe qPCR) 12.5μL 1× Upstream primer (F) (10μmol / L) 0.5μL 0.2 µmol / L Downstream primer (R) (10µmol / L) 0.5μL 0.2 µmol / L Probe (P) (10µmol / L) 0.5μL 0.2 µmol / L DNA (10-100ng / μL) 2 μL (0.8-8ng) wxya 2 o

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Abstract

The invention relates to a method for identifying and detecting boletus bainiugan dentinger or componentsof the boletus bainiugan dentinger. Primers used in the detection method comprise: an upstreamprimer (F) 5'-GGATCATTATCGAGTTAGAC-3', and a downstream primer (R) 5'-TGGACATGCAATAGAGTA-3'. The method has the beneficial effects that 1) due to adoption of a fluorescent PCR (polymerase chain reaction) detection method, the technical defect that some items cannot be detected by mass spectrometry during custom clearance can be overcome; 2) the technical method provided by the invention has a significant advantage of short detection time; 3) the method has very low requirements on masses and concentrations of detected items, and thus goods, commodities or items barely have amount loss; 4) identification results obtained by using the primers and probes provided by the invention are very accurate, the blank of identification of delicious porcini and components of products of the delicious porcini in China can be made up, and although the invention only provides the method for identifying the boletus bainiugan dentinger, scientific research precedent of fluorescent PCR identification methods of other delicious porcini can be developed on the basis; and 5) the invention can provide necessary technical support for Chinese customs to perform relevant tasks.

Description

technical field [0001] The invention belongs to the technical field of biological identification, in particular to the technical field of a fluorescent PCR identification and detection method suitable for the wild fungus species in Yunnan: boletus albicans. Background technique [0002] The unique climate and soil conditions make Yunnan extremely rich in wild fungus resources. In the international market, the mushrooms with huge order demand are: white boletus, matsutake, truffle, morel, chanterelle. The main foreign markets for wild mushroom products in Yunnan are Japan and the European Union. Among the categories of products sold, the main export product of the EU is white boletus ( Boletus bainiugan Dentinger), commonly known as delicious boletus. Yunnan's delicious boletus occupies a very large proportion in the overall wild mushroom industry chain and is the main product of foreign trade. [0003] When performing the task of guarding the security of the country and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12Q1/686C12Q1/04C12N15/11C12R1/645
CPCC12Q1/6895C12Q1/686C12Q2563/107
Inventor 陈丽萍杨玲春丁元明李云飞王英张银张薇高山陈宏仙陈芸殷红李芳范效英吕廷保刘燕
Owner 昆明海关技术中心
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