Method for purifying mononuclear bacterial strain of needle mushroom

A technique for velutipes and mushrooms, which is applied to the field of purification of monokaryotic strains in the cultivation and breeding of enoki mushrooms, can solve problems such as being difficult to distinguish, and achieve the effect of reliable guarantee and avoiding adverse effects.

Inactive Publication Date: 2013-11-20
上海永大菌业有限公司
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  • Abstract
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AI Technical Summary

Problems solved by technology

However, heterokaryotes can be formed between sexually incompatible monokaryotic strains, and such heterokaryotes will

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0016] Prepare PDA medium:

[0017] Recipe: peeled potato 200g boiled juice, glucose 20g, agar 20g, Tween (80) 0.1ml, distilled water 1000ml, pH 6.5-6.8, pour the above PDA culture-based plate, 18-20ml per plate, it becomes the band Plates of PDA medium.

[0018] Purification culture:

[0019] Cultivate Flammulina velutipes strains (provided by the Edible Fungi Institute of Shanghai Academy of Agricultural Sciences) to produce mushrooms, select complete and well-growing fruiting bodies of Flammulina velutipes, pick the caps and place them on a sterile plate, seal the sterile plate and place it in an environment of 18-19°C After 24 hours, take out the cap in the ultra-clean workbench, and a spore print is formed on the sterile plate. At this time, the collection of basidiospores is completed, and 10ul sterile distilled water is pipetted on the spore print and repeated for 10-15 hours. Once, draw the spore liquid, transfer it to a 1ml centrifuge tube, dilute 5 to 8 gradients, ...

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Abstract

The invention relates to a method for purifying a mononuclear bacterial strain of a needle mushroom. The method for purifying the mononuclear bacterial strain of the needle mushroom is characterized by including the steps that basidiospores collected from the sporocarp of the needle mushroom are grafted to a flat plate with PDA culture media to be cultivated so as to form a circular bacterial colony, the hypha of 1-2mm with PDA culture media in a connecting mode can be picked on the edge of the bacterial colony to be used as a grafting block, the hypha is grafted to a novel flat plate with PDA culture media, cultivation is conducted until a radial pattern hypha grows out around the grafting block, a single hypha is picked again to put into novel PDA culture media to be cultivated, cultivation is carried out continuously for 6-7 days so that a purified bacterial colony can be obtained, and the hypha is selected at will to be cultivated again to obtain the purified bacterial strain of the needle mushroom. The formula of the PDA culture media is that 20g of peeled potatoes are used for conducting juice boiling, 20g of glucose, 20g of agar and 1,000ml of distilled water with the twain (80) being 0.1ml and the pH value being 6.5-6.8 are poured onto the flat plate, and 18-20m of the mixing liquid exists on each plat plate. By means of the method for purifying the mononuclear bacterial strain of the needle mushroom, the highly-purified mononuclear bacterial strain can be obtained.

Description

technical field [0001] The invention relates to a method for cultivating edible fungi, and in particular discloses a method for purifying monokaryotic strains in the cultivation of Flammulina velutipes, which is applied to the factory production of Flammulina velutipes. Background technique [0002] At present, China's edible mushroom production accounts for 70% of the world's total output, with an output of more than 10 million tons, and China has become the world's largest mushroom producer. With the increasing demand for edible fungi in domestic and foreign markets, the edible fungus industry in my country has developed rapidly. In 1978, China's fungi output was only 60,000 tons, accounting for 6% of the world's total output. The total national edible fungi output soared from 400,000 tons in 1978 to 10.38 million tons in recent years. According to statistics from the Ministry of Agriculture, in the domestic planting industry, the output value of edible fungi ranks sixth ...

Claims

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Application Information

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IPC IPC(8): A01G1/04
Inventor 张旬旬黄聿长
Owner 上海永大菌业有限公司
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