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Application of bimolecular marker in ganoderma lucidum strain breeding, breeding and planting method and ganoderma lucidum strain

A planting method and bimolecular technology, applied in botany equipment and methods, microbial-based methods, applications, etc., can solve the problems of low basidiospore binding rate and long breeding cycle

Pending Publication Date: 2022-04-12
CHONGQING ACAD OF CHINESE MATERIA MEDICA +1
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  • Summary
  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to solve the problem of low combination rate and long breeding cycle between basidiospores in the existing Ganoderma lucidum breeding, and to provide a method of using MIP and cla4 genotypes to breed Ganoderma lucidum strains that shortens the breeding cycle The application of Ganoderma lucidum hybrid strain and the cultivation method

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1: Breeding of ganoderma lucidum hybrid strain

[0030] The present invention utilizes MIP and cla4 genotype in the method for selection and breeding of ganoderma lucidum hybrid strain, and its steps are,

[0031] 1) Obtain a single basidiospore: Ganoderma lucidum strains found in the wild, use a sterilization bag to collect the Ganoderma lucidum spore powder, weigh 1g of the collected Ganoderma lucidum spore powder into 99mL sterile water, shake well, suck 1mL with a straw and transfer it to 99mL again in sterile water, repeat this until the concentration of basidiospores in the basidiospore suspension is 10 1 -103 Individual / mL, diluted basidiospore suspension, that is, to obtain a single basidiospore;

[0032] 2) Obtain a monospore community: apply and culture the basidiospore suspension obtained in step 1), and the medium used is PDA enriched medium No. Add 10-50 grams of wood chips, add 500ml of distilled water, boil for 20 minutes, filter, take the fi...

Embodiment 2

[0037] Add step 6 on the basis of Example 1)

[0038] 6) Perform genotype identification of the multiple hybrid strains obtained (identification method is the same as step 3), and select hybrid strains that all contain MIP genotype and cla4 genotype to obtain excellent hybrid strains.

Embodiment 3

[0039] Embodiment 3: replace the MIP genotype in Embodiment 1 and Embodiment 2 with HD1 gene or HD2 gene; replace the cla4 genotype with GL31781 gene or GL31782 gene.

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Abstract

The invention relates to the field of medicinal fungus breeding, and discloses application of a bimolecular marker in ganoderma lucidum strain breeding, a breeding and planting method and a ganoderma lucidum strain, including application of the bimolecular marker, MIP and cla4 genotypes in the breeding and planting method of a ganoderma lucidum hybrid strain, the breeding and planting method and obtained ganoderma lucidum sporocarp and spore powder. The invention aims to solve the problems of low binding rate of free pairing between basidiospore and long breeding period in the existing ganoderma lucidum breeding, and provides the application of MIP and cla4 genotypes in ganoderma lucidum strain breeding for shortening the breeding period, and the obtaining and planting method of the ganoderma lucidum hybrid strain.

Description

technical field [0001] The invention relates to the field of medicinal fungus breeding, in particular to the application, acquisition and planting method of a bimolecular marker in the breeding of ganoderma lucidum strains and the ganoderma lucidum strain. Background technique [0002] Ganoderma lucidum is the general term of Ganoderma in a broad sense, belonging to Kingdom Fungi, Basidiomycota, Basidiomycetes, Ganodermatales, Ganodermaceae (Kingdom Fungi) in taxonomy. Ganodermataceae), whose use history can be traced back to the Neolithic Age (Hemudu Culture Period) 6,800 years ago, is a famous traditional Chinese medicinal material in my country. Traditional Chinese medicine has long regarded it as a treasure for nourishing and strengthening, strengthening the foundation and strengthening the body. Ancient and modern pharmacological and clinical studies have proved that Ganoderma lucidum has the functions of nourishing qi and blood, calming the mind and strengthening the s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12N1/14A01G18/00A01G18/40C12R1/645
Inventor 贺宗毅陈仕江王伯初郝石磊李卿张德利邢康康游华建罗昌树谭发银
Owner CHONGQING ACAD OF CHINESE MATERIA MEDICA
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