Wild rice stem smut fungus strain capable of producing anti-fungus antibiotic and its use

An antibiotic and antifungal technology, applied in the direction of fungi, applications, biocides, etc., can solve the problems of destroying ecological balance and killing natural enemies

Inactive Publication Date: 2008-03-05
GUIZHOU UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In addition, chemical pesticides kill a large number of natural enemies and destroy the ecological balance

Method used

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  • Wild rice stem smut fungus strain capable of producing anti-fungus antibiotic and its use

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Embodiment Construction

[0013] (1) the separation and purification method of Zizania esculenta UEZC1 of the present invention:

[0014] Rinse the old cooked Zizania zizania with tap water to remove the dirt on the surface, dry it, and wipe the surface with 75% alcohol. After the alcohol is evaporated, cut it longitudinally with a sterile knife, take an appropriate amount of spore powder and add it to a triangular flask with glass beads and 0.05% Tween 80 aqueous solution, fully break up the teliospores, and the final concentration is 2×10 4 / ml to obtain a uniform suspension of teliospores. Take 50 μl teliospore suspension and coat PDA agar plate or water agar plate, then observe the plate coated with teliospore suspension under a microscope, and mark the position where there is only a single teliospore. Cultivate at a constant temperature of 28°C for 4 to 7 days, transfer the colony grown from the marked place to a PDA slant, and cultivate at a constant temperature of 28°C for 5 days to obtain a pu...

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Abstract

The present invention discloses a kind of Ustilago esculenta strain UEZC1 capable of producing antifungal antibiotic and its use. The Ustilago esculenta strain UEZC1 features that the teleutospore is germinated on potato glucose liquid culture medium to form basidium promycelium with 1-4 basidiospore promycelia, and the basidiospore after falling off further grow to form fusiform short hypha for inserting basidiospore. The present invention can produce antifungal antibiotic, and may be used in developing and producing medical and agricultural antifungal antibiotic.

Description

technical field [0001] The invention relates to a Zizania smut strain capable of producing antifungal antibiotics and its application. Background technique [0002] The implementation of the agricultural sustainable development strategy has aroused people's attention to the dangers and harms of chemical pesticides. Therefore, biopesticides that are environmentally friendly, healthy, efficient, safe, and environmentally friendly have become the subject of current ecological agriculture research. With the rise of global biotechnology, biopesticide has developed rapidly and has become an industry with recent economic and social benefits. For agricultural antibiotics, dozens of antibiotics such as effectivemycin, kasugamycin, polyoxin, and blasticidin have been used in Japan, and many varieties such as Avermectin and Gibberllin have been put into production in the United States. Kazumycin, griseofulvin, Antimycotics, Phytomycin, etc. So far, more than 900 kinds of antibiotics...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12P1/02A01N63/04
Inventor 周礼红程龙梁宗琦
Owner GUIZHOU UNIV
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