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Method for efficiently acquiring basidiospore homocaryon of agaricus bisporus

A technology for Agaricus bisporus and basidiospores, which is applied to the field of efficiently obtaining basidiospore homokaryons of Agaricus bisporus, can solve problems such as the scarcity of homokaryons, time-consuming and laborious separation of single spores, and improve efficiency, reduce labor intensity, The effect of improving worker efficiency

Active Publication Date: 2018-12-11
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The single spore separation method selects the single spore homokaryon by observing the growth speed and shape of the isolated basidiomycetes. Due to the rare number of homokaryotic bodies, according to statistics, the homokaryotic spores account for about 5.6% of the total number of spores, so the isolation of single spores is time-consuming and laborious.
The prior art lacks a method for efficiently obtaining the basidiospore homokaryon of Agaricus bisporus

Method used

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  • Method for efficiently acquiring basidiospore homocaryon of agaricus bisporus
  • Method for efficiently acquiring basidiospore homocaryon of agaricus bisporus
  • Method for efficiently acquiring basidiospore homocaryon of agaricus bisporus

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Embodiment 2

[0086] The difference between this embodiment and Example 1 is that the planting facilities are different. Using the planting facilities in this embodiment can greatly improve the work efficiency of the method, and obtain Agaricus bisporus 17 basidiospore homokaryon more efficiently. As a preference, The method provided by the present invention is further configured as follows: the above-mentioned planting facility is spliced ​​by a plurality of planting components, the above-mentioned planting component includes a bottom plate 1, a mushroom seeding box 2 is longitudinally arranged in the center of the bottom plate 1, and mushrooms 17 are planted in the above-mentioned seeding box 2 . It is used for planting the mushrooms 17 at intervals in rows, which is convenient for detection and harvesting.

[0087] The above-mentioned mushroom 17 is suspended with a lead screw 9, and the two ends of the lead screw 9 are provided with support seats vertically connected with the base plate...

Embodiment 1 and Embodiment 2

[0094] In conjunction with embodiment 1 and embodiment 2, set forth research method as follows:

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Abstract

The invention discloses a method for efficiently acquiring basidiospore homocaryon of agaricus bisporus. The method comprises: step one, carrying out agaricus bisporus planting; to be specific, selecting agaricus bisporus types and planting and cultivating samples in agaricus bisporus planting equipment; step two, carrying out sampling; to be specific, at an agaricus bisporus picking stage, carrying out agaricus bisporus sampling at five developmental stages including a veil non-appearing stage, a veil initial appearing stage, a veil inward recession stage, a veil flattening stage, and a veilfracture stage based on a time sequence; step three, carrying out analysis; to be specific, selecting mediotrastum of agaricus bisporus at different development stages, carrying out statistics of thenumber of basidia with sporophore growing at mediotrastum at different development stages as well as the proportions of different basidia; step four, determining an optimal obtaining period; to be specific, determining at least one development stage as an optimal obtaining period based on the result from the step three; and step five, picking basidiospore homocaryon in batches. According to the invention, the basidiospore homocaryon of agaricus bisporus is obtained at the veil initial appearing stage of the agaricus bisporus, so that the obtaining efficiency is improved substantially.

Description

technical field [0001] The invention relates to the technical field of mushroom cultivation and improvement, in particular to a method for efficiently obtaining the basidiospore homokaryon of Agaricus bisporus. Background technique [0002] Agaricus bisporus (Lange ) Sing, also known as Agaricus bisporus, mushroom (Taiwan). It belongs to Eumycota, Basidiomycetes, Agaricales, Agariceae, Agaricus taxonomically. [0003] Agaricus bisporus is the most widely cultivated edible fungus with the highest yield and largest consumption in the world, and has important economic value. Since the introduction of cultivation in the 1930s in my country, cultivation has been promoted throughout the country, among which provinces such as Fujian, Shandong, Henan and Zhejiang have more planting areas. Agaricus bisporus is a species of different natural populations with different mating-type life histories. The dual life history of heterothalism. The three life histories are mainly divided ac...

Claims

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Application Information

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IPC IPC(8): A01G18/00
CPCA01G18/00
Inventor 赵建霞
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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