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Gjaponicum basidiospore germination medium and germination method of gjaponicum basidiospore

A germination medium and a technology for basidiospores are applied in the field of germination medium for basidiospores of Zizhi and the germination of basidiospores of Zizhi, which can solve the problems of difficulty in obtaining monokaryotic strains, difficult germination, breeding of Zizhi varieties, etc., and achieve easy large-scale popularization. Simple application, simple method, simple composition effect

Active Publication Date: 2019-06-18
SHANGHAI ACAD OF AGRI SCI +1
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the basidiospores of Zizhi that have not germinated cannot produce primary hyphae, so it is impossible to breed Zizhi varieties by means of hybridization, protoplast fusion or mutagenesis.
Zizhi spores have two layers of walls, which are very tough, and it is difficult to break them by general chemical and physical methods; it is very difficult to germinate in nature, and the germination rate is extremely low under laboratory conditions
Thereby restricting the breeding of new varieties of Zizhi
[0004] Although the use of protoplast monokaryonization technology can solve the problem that the germination rate of the basidiospores of Zizhi is extremely low and the monokaryotic strains are not easy to obtain, but the monokaryotic strains obtained by the protoplast monokaryonization technology do not have meiosis. The resulting monokaryotic strains were not rich in genetic diversity
Therefore, it is difficult to obtain new strains with extensive variation through protoplast mononuclearization technology

Method used

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  • Gjaponicum basidiospore germination medium and germination method of gjaponicum basidiospore

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Embodiment 1

[0030] The present embodiment provides a germination medium for the basidiospores of Zizhi lucidum, each 1000mL of the germination medium includes the following components: 200g of potatoes, 15g of glucose, 5g of sucrose, MgSO 4 1.1g, KH 2 PO 4 0.5g, 1.7g agar powder, 170mL of Zizhi boiled liquid, 65μL of glacial acetic acid.

[0031] The preparation method of the Zizhi boiled liquid is as follows: the Zizhi fruiting bodies are boiled in boiling distilled water for 13 minutes, filtered after cooling, the filtrate is the Zizhi boiled liquid, and the solid-to-liquid ratio of the Zizhi fruiting bodies to distilled water is 1:21 .

[0032] The preparation method of the Zizhi basidiospore germination medium is as follows: mix potatoes, glucose, sucrose, MgSO4, KH2PO4, agar powder, Zizhi boiled liquid and distilled water according to the formula, then sterilize at 120°C for 20 minutes, and then add the formula quantity of ice Acetic acid, obtains the described Zizhi basidiospor...

Embodiment 2

[0034] The present embodiment provides a germination medium for the basidiospores of Zizhi lucidum, each 1000mL of the germination medium includes the following components: 180g of potatoes, 12g of glucose, 4g of sucrose, MgSO 4 1g, KH 2 PO 4 0.4g, 1.6g agar powder, 160mL of Zizhi boiled liquid, 60μL of glacial acetic acid.

[0035] The preparation method of the Zizhi boiled liquid is as follows: the Zizhi fruiting bodies are boiled in boiling distilled water for 12 minutes, filtered after cooling, the filtrate is the Zizhi boiled liquid, and the solid-to-liquid ratio of the Zizhi fruiting bodies to distilled water is 1:20 .

[0036] The preparation method of the Zizhi basidiospore germination medium is as follows: mix potatoes, glucose, sucrose, MgSO4, KH2PO4, agar powder, Zizhi boiled liquid and distilled water according to the formula, then sterilize at 120°C for 20 minutes, and then add the formula quantity of ice Acetic acid, obtains the described Zizhi basidiospore ...

Embodiment 3

[0038] The present embodiment provides a germination medium for the basidiospores of Zizhi lucidum, each 1000mL of the germination medium includes the following components: 210g of potatoes, 16g of glucose, 6g of sucrose, MgSO 4 1.2g, KH 2 PO 4 0.6g, 1.8g agar powder, 180mL of Zizhi boiled liquid, 70μL of glacial acetic acid.

[0039] The preparation method of the Zizhi boiled liquid is as follows: the Zizhi fruiting bodies are boiled in boiling distilled water for 14 minutes, filtered after cooling, the filtrate is the Zizhi boiled liquid, and the solid-to-liquid ratio of the Zizhi fruiting bodies to distilled water is 1:22 .

[0040] The preparation method of the Zizhi basidiospore germination medium is as follows: mix potatoes, glucose, sucrose, MgSO4, KH2PO4, agar powder, Zizhi boiled liquid and distilled water according to the formula, then sterilize at 120°C for 20 minutes, and then add the formula quantity of ice Acetic acid, obtains the described Zizhi basidiospor...

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Abstract

The invention belongs to the technical field of edible fungus breeding, and relates to a gjaponicum basidiospore germination medium and a germination method of gjaponicum basidiospore. Every 1,000 mLof the germination medium is prepared from 160-240 g of potatoes, 10-20 g of glucose, 3-8 g of saccharose, 0.8-1.5 g of MgSO4, 0.3-0.8 g of KH2PO4, 1.5-2 g of agar powder, 150-200 mL of gjaponicum cooked liquid, 40-80 microliters of glacial acetic acid and the balance distilled water. According to the gjaponicum basidiospore germination medium, the glacial acetic acid with the specific content isadded, the glacial acetic acid cooperates with the potatoes, the glucose, the saccharose, MgSO4, KH2PO4, the agar powder and the gjaponicum cooked liquid, and the germination rate of the gjaponicum basidiospore is increased to 6.50%. The method for germinating the gjaponicum basidiospore with the gjaponicum basidiospore germination medium is simple, easy to operate and easy to apply and popularizeon a large scale.

Description

technical field [0001] The invention belongs to the technical field of edible fungus breeding, and relates to a germination medium and a germination method of basidiospores of edible fungi, in particular to a germination medium and a germination method of basidiospores of Zizhi. Background technique [0002] Zizhi belongs to Basidiomycotina, Phytomycetes, Polyporaceae, Ganodermaceae, and Ganoderma genus. Zizhi is flat and warm. Non-toxic, slightly bitter, without any side effects. It is mainly used to treat diabetes, high blood pressure, coronary heart disease, insomnia and other diseases. Long-term use can resist tumors, protect the liver, delay aging, strengthen the body, and is beneficial and harmless to the human body. It is suitable for long-term health care. [0003] As a species of Ganoderma lucidum, Zizhi is also a quadrupolar heterothallic combination, that is, two different nuclei coexist in the hyphae of one cell to undergo reproductive growth and form fruiting ...

Claims

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Application Information

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IPC IPC(8): A01G18/20A01G18/40
Inventor 唐传红张劲松王晨光杨焱唐庆九刘艳芳鲍大鹏周靖张赫男谭贻贾薇周帅张忠冯娜冯杰吴迪汪雯翰颜梦秋庄海宁刘方
Owner SHANGHAI ACAD OF AGRI SCI
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