Method for germinating ganoderma lucidum (leyss.fr) karst basidiospores

A technology of basidiospores and spores, which is applied in the fields of botanical equipment and methods, horticulture, and application to achieve the effects of stable genetic traits, rich genetic diversity and rich diversity in offspring

Inactive Publication Date: 2013-04-10
FUJIAN AGRI & FORESTRY UNIV
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AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a method for the germination of Chizhi basidiospores, to solve the difficult problem that Chizhi basidiospores are not easy to germinate, and to lay the foundation for the single-spore hybrid breeding of Chizhi

Method used

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  • Method for germinating ganoderma lucidum (leyss.fr) karst basidiospores
  • Method for germinating ganoderma lucidum (leyss.fr) karst basidiospores

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Embodiment 1

[0036] Embodiment 1: A kind of method of red sesame basidiospore germination

[0037] 1. Parental selection and breeding:

[0038] Japanese Chizhi and Chizhi 5.75 with good production characteristics were selected as parents, activated on the PDA slope, and then transferred to the wood dust bag after the slope was covered. See literature for specific planting methods: Zeng Zhenji, Gu Peizong, Chen Dongbiao, Ling Hongtong, He Huanqing. Cultivation technology of red Ganoderma lucidum with miscellaneous sawdust[J]. Chinese Edible Fungi, 2004, 23(3): 41-42.

[0039] 2. Basidiospore collection period and method

[0040] The release cycle of Chizhi spores is about 30 days, and the basidiospores used for germination should be selected to be released in the first 10 days. That is, when the red lucidum cap is differentiated and formed, select the fruit body to grow to 3-4cm, and collect the basidiospores when the edge of the cap is 1-2cm in white growth circle. Use a sterile whit...

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Abstract

The invention relates to a method for germinating ganoderma lucidum (leyss.fr) karst basidiospores. A ganoderma lucidum (leyss.fr) karst fruiting body period is selected, a bagging method is used for collecting the basidiospores, basidiospore collection time is controlled, the basidiospores are germinated in a malt ganoderma lucidum (leyss.fr) karst culture medium, and the germination rate of the ganoderma lucidum (leyss.fr) karst basidiospores is over 1.0 percent, so that monosporous strains of ganoderma lucidum (leyss.fr) karst are obtained. A formula for the malt ganoderma lucidum (leyss.fr) karst culture medium comprises 200 milliliters of malt wort, 100 milliliters of ganoderma lucidum (leyss.fr) karst extract, 20 grams of agar and 700 milliliters of water, and the time of sterilization at 121 DEG C is 30 minutes. According to the method, the problem of low germination rate of the ganoderma lucidum (leyss.fr) karst basidiospores under a laboratory condition is solved, so that a foundation is laid for the cross breeding of monospores of the ganoderma lucidum (leyss.fr) karst. The method is simple and easy, the use of special medicaments and apparatuses is avoided, and the method can be used for obtaining required monosporous strains for the cross breeding of the ganoderma lucidum (leyss.fr) karst or genetic analysis on the ganoderma lucidum (leyss.fr) karst in an ordinary laboratory.

Description

technical field [0001] The invention relates to a method for germination of Chizhi basidiospores. Background technique [0002] Chizhi, scientific name Ganoderma lucidum (Leyss.Fr) Karst, belonging to the Phylum Fungi, Basidiomycotina, Phytomycetes, Phyllomycetes, Ganodermaceae, and Ganoderma genus. The earliest pharmacy book in my country (Shen Nong’s Materia Medica) records that Chizhi is "bitter, flat, and non-toxic", and it is mainly used to treat "chest knots", "benefits the heart, nourishes the middle, increases wisdom, and does not forget". Food does not grow old, prolongs life and immortality." It can be seen that the use of Ganoderma lucidum was known as early as the first century AD. In the 1970s, Lin Zhibin and others put forward the working hypothesis of the mechanism of action of Ganoderma lucidum "strengthening the body and strengthening the root" in the process of pharmacological and clinical research on Ganoderma lucidum. And prove and perfect this hyp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04
Inventor 吴小平柯斌榕刘芳刘新锐
Owner FUJIAN AGRI & FORESTRY UNIV
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