Black fungus strain JH40 and application thereof

A technology of JH40, black fungus, applied in the fields of application, horticulture, botanical equipment and methods, etc., can solve the problems of runny ears and ear pieces falling off, light color of black fungus, poor quality, etc., and achieve short growth cycle, ear pieces Thickness and high foaming rate, high biological efficiency

Active Publication Date: 2021-03-30
BIOLOGY INST OF HEBEI ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the problems that the existing black fungus varieties grow too fast under the condition of 30°C or higher, resulting in light color, thin flesh

Method used

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  • Black fungus strain JH40 and application thereof
  • Black fungus strain JH40 and application thereof
  • Black fungus strain JH40 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0032] 1 Materials and methods

[0033] 1.1 Materials

[0034] 1.1.1 Enzyme solution

[0035] Lysozyme: purchased from Guangdong Institute of Microbiology.

[0036] Helicase: purchased from Beijing Suolaibao Technology Co., Ltd., dissolved in 0.6M mannitol and sterilized with a 0.22 μm microporous membrane for use.

[0037] 1.1.2 Test medium

[0038] Solid PDA medium (1L): 200g of peeled potatoes, cut into small pieces and boiled for half an hour, then filtered through gauze, added 20g of glucose and 15g of agar, divided into 250mL Erlenmeyer flasks, at natural pH, sterilized at 115°C for 30min.

[0039] Liquid PDA medium (1L): 200g of peeled potatoes, cut into small pieces and boiled for half an hour, then filtered through gauze, added 20g of glucose, the pH was natural, divided into 250mL Erlenmeyer flasks, sterilized at 115°C for 30min.

[0040] NA medium (1L): glucose 10g, peptone 5g, beef extract 3g, yeast extract 1g, agar 15g, with ddH 2 Dilute O to 1L, pH 6.8-7.0, ...

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Abstract

The invention relates to the technical field of edible fungus cultivation, and particularly discloses a black fungus strain JH40 and application thereof. The strain preservation number of the black fungus strain JH40 is CCTCC No: M20191058. The invention also provides protoplasts, basidiospores, mycelia and sporophores generated by the black fungus strain JH40. The black fungus strain JH40 provided by the invention can be applied to black fungus cultivation and health care product development. Compared with a traditional black fungus strain, the black fungus strain JH40 provided by the invention has stronger environmental adaptability, resists high temperature, can normally grow in an environment of 30 DEG C or above, and has the excellent qualities of a short growth period, high biological efficiency, good genetic stability, thick auricle, high soaking rate and the like.

Description

technical field [0001] The invention relates to the technical field of edible fungus cultivation, in particular to a black fungus strain JH40 and its application. Background technique [0002] Black fungus (Auricularia auricula) is fresh and crisp, smooth and refreshing. It is rich in various amino acids, trace elements and elastic jelly. It contains functional nutrients such as polysaccharides, melanin, polyphenols, flavonoids, etc., which can help digestion , prevent thrombosis, arteriosclerosis, iron deficiency anemia and coronary heart disease, etc., is a nutritious and pharmacological edible fungus. [0003] my country is a big producer of black fungus, and the black fungus output accounts for more than 99% of the world's total output. Black fungus products are dried and stored, and the price is stable. The development of this industry is also of great significance to the rural revitalization of our country. In the process of black fungus cultivation, a large amount o...

Claims

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Application Information

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IPC IPC(8): A01G18/50
CPCA01G18/50
Inventor 刘昆昂张根伟马宏尹淑丽刘振国刘萌付艳菊李书生
Owner BIOLOGY INST OF HEBEI ACAD OF SCI
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