Method for collecting clean basidiospores of agaricales fungi

Abstract

The invention discloses a method for collecting clean basidiospores of toadstool fungi. The method comprises: wiping the surface of a fruit body by dipping 70% ethanol with a cotton ball, cutting offthe stipe from the joint between the stipe and the cap, and placing the cap upside down on a super clean bench; folding the sulfuric acid paper twice, then cutting off the right-angle part, spreadingand placing the sulfuric acid paper into the bottom of a petri dish, taking two autoclavable rubber bands to sleeve the petri dish, placing the cap on the rubber bands, with gills or pores facing down, and covering the petri dish to stand for 4 to 24 hours; removing the cap of the fruit body, removing the rubber bands, folding the sulfuric acid paper inward twice, so that the spore-bearing side islocated on the inner side, spreading the sulfuric acid paper into a funnel shape, placing the same above the mouth of a centrifuge tube, and washing the inside of the sulfuric acid paper uniformly with sterile water to collect the basidiospores. The method of the invention can obtain clean basidiospores, has a high spore recovery rate and is simple to operate.

Description

technical field [0001] The invention belongs to the field of biological technology, and in particular relates to a method for collecting clean basidiospores of agaric fungi. Background technique [0002] Basidiospores are important materials for the isolation and cultivation of agaric fungi, genetic improvement and ecological habit research, and their morphological characteristics are also an important basis for the identification of agaric fungi. The collection of pure basidiospores is extremely critical for the study of agaric fungi. At present, the collection methods of agaric fungus basidiospores mainly include bagging collection method, spore print collection method, ejection collection method and so on. In terms of collection methods of basidiospores, Li Rongtong mentioned in "Acta Edible Fungi Sinica" 2010 17(1): 44-47 "Collection and Germination Conditions of Sclerotia Pleurotus Basidiospores" that the use of gill-fold washing method to collect sclerotia Pleurotus...

AI Technical Summary

Problems solved by technology

In terms of collection methods of basidiospores, Li Rongtong mentioned in "Acta Edible Fungi Sinica" 2010 17(1): 44-47 "Collection and Germination Conditions of Sclerotia Pleurotus Basidiospores" that the use of gill-fold washing method to collect sclerotia Pleurotus spores Spores, in this method, the gills are in direct contact with the spore collection liquid, which increases the probability of contamination. In addition, this method is not suitable for the collection of basidiospores of the genus Boletus agaricus containing bacteria pores

Chinese Patent Publication No. CN103688746A disclosed "A Method for Collecting and Cultivating Edible Mushroom Spores" on April 02, 2014. This method is a traditional spore printing method. Since the sporocarp is in contact with the collection paper during the spore collection process, it is difficult to ensure collection. The cleanliness of the spores, in addition the spores collected by the collection paper are difficult to transfer; Chinese Patent Publication No. CN105039178A disclosed on November 11, 2015 "a method for the germination of the basidiospores of Dictyostelium oberatus". The solid tissue adheres to the inner wall of the test tube, so it is difficult to ensure the cleanliness of the spores; in addition, due to the diameter of the test tube, most spores of the fruiting bodies cannot be collected; at the same time, it is difficult to transfer the spores collected in the test tube; Chinese Patent Publication No. CN 106754624A disclosed "a method for the germination of Ganoderma lucidum basidiospores" on May 31, 2017. The collection environment of this method is relatively closed, and the fruiting body is easy to rot, so it is not suitable for the collection of agaric fungus basidiospores; There are many physical contacts, and it is difficult to obtain clean basidiospores