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High-efficiency phosphate-solubilizing penicillium and application thereof in promoting growth of Chinese red pines

A technology of Pinus massoniana and Penicillium, applied in the field of microorganisms, can solve the problems of less research on phosphorus-solubilizing fungi, and achieve excellent strain resources, good application and development prospects, and the effect of promoting growth

Active Publication Date: 2013-05-22
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few studies on phosphate-solubilizing fungi

Method used

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  • High-efficiency phosphate-solubilizing penicillium and application thereof in promoting growth of Chinese red pines
  • High-efficiency phosphate-solubilizing penicillium and application thereof in promoting growth of Chinese red pines
  • High-efficiency phosphate-solubilizing penicillium and application thereof in promoting growth of Chinese red pines

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1JP-NJ4 bacterial strain is to the phosphorus dissolving test of insoluble phosphate

[0027] Inoculate the Penicillium pinophilum JP-NJ4 strain on the PDA plate, and after culturing for 7 days, take the bacterial block (diameter 6mm) with a sterilized hole puncher, and inoculate it into 50mL NBRIP liquid medium and liquid medium with different phosphorus sources respectively. In the 100mL Erlenmeyer flasks of A, B, and C, inoculate 6 bacterial blocks in each flask, and use the same volume of non-inoculated phosphate-dissolving medium as the control (CK). Three replicates were set up for each treatment. 25℃, 200r·min -1 After 7 days of shaking culture, the fermentation broth was centrifuged for 10 min (4°C, 10000r min -1 ); using the molybdenum-antimony anti-colorimetric method, that is, take 10mL of the supernatant obtained by the above centrifugation and add it to a 50mL volumetric flask, then add about 25mL of deionized water, and add 1~2 drops of 2,4-dinit...

Embodiment 2

[0033] The dynamic change of embodiment 2JP-NJ4 strain phosphorus solubilizing ability

[0034] The JP-NJ4 strain was first activated on the NBRIP medium, and then activated twice on the PDA. After the JP-NJ4 strain grew on the PDA for 7 days, the bacterial block with a diameter of 6 mm was taken from the medium and inoculated in 50 mL of NBRIP Medium 100mL Erlenmeyer flask, 25°C, 200r.min -1 Shaking culture, set 7 replicates, with blank medium as the control. Every 24 hours, take the fermentation broth, and centrifuge the fermentation broth for 10min (4°C, 10000r.min -1 ), take 100uL of the supernatant obtained by centrifugation and add it to a 50mL volumetric flask, then add about 25mL of deionized water, then add 1-2 drops of 2,4-dinitrophenol indicator, and then add 5mL of molybdenum-antimony antimony Chromogenic agent, add deionized water to make up volume, mix well, after standing for half an hour, measure OD value. Then the phosphorus solubilizing ability of the stra...

Embodiment 3

[0036] The pot experiment that embodiment 3JP-NJ4 bacterial strain affects the growth of Pinus massoniana

[0037] The JP-NJ4 strain was first activated on the NBRIP medium, and then activated twice on the PDA, the bacterial block with a diameter of 6 mm was taken and inoculated into a 100 mL Erlenmeyer flask containing 50 mL of PD medium, at 25 ° C, 200 r. min -1 Shaking culture 7d. Fermentation broth (4°C, 6000r.min -1 ) centrifuged for 5min, the mycelium retained after centrifugation was crushed with a magnetic stirrer, and the bacterial suspension was adjusted with sterile physiological saline (7~8×10 8 cfu.mL -1 ) to make fungicide.

[0038] The JP-NJ4 strain inoculation test of Pinus massoniana seedlings was divided into four treatments, and each treatment was applied with 15mL of the corresponding substance, A: bacterial suspension, B: supernatant obtained after centrifugation of the fermentation broth, C: blank medium (PD ), D: sterile saline (CK). There were 20 ...

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Abstract

The invention discloses a high-efficiency phosphate-solubilizing penicillium and application thereof in promoting the growth of Chinese red pines. The classification designation of the high-efficiency phosphate-solubilizing penicillium is Penicillium pinophilum JP-NJ4; and the Penicillium pinophilum JP-NJ4 was collected at China Center for Type Culture Collection in Wuhan university, Wuhan, China on May 14, 2012, and the collection number is CCTCC NO:M2012167. The Penicillium pinophilum JP-NJ4 has the following advantages: the Penicillium pinophilum JP-NJ4 has high solubility on insoluble inorganic phosphate such as tricalcium phosphate (Ca3(PO4)2), aluminum phosphate (AlPO4) and calcium hydrogen phosphate (CaHPO4.2H2O); and pot incubation tests verify that the growth of Chinese red pines can be obviously promoted after Chinese red pine seedlings are inoculated with the JP-NJ4 microbial inoculum. Thus, the Penicillium pinophilum JP-NJ4 can provide an excellent strain resource for the development of a microbial bacterial fertilizer, and has favorable application development prospects.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a high-efficiency Penicillium pinophilum JP-NJ4 and its application in promoting the growth of Pinus massoniana, in particular to Penicillium pinophilum JP-NJ4 . Background technique [0002] Masson pine is the most representative afforestation tree species in southern my country, and also a pioneer tree species in forest succession. However, at present, the masson pine forest in southern my country is facing a serious problem of soil fertility decline, resulting in poor growth and biomass decline of the masson pine forest. In the process of masson pine woodland decline, insufficient phosphorus supply is a prominent and common problem. In forestry, phosphorus has a greater impact on seedling quality, especially in coniferous species. Adequate forest supply can accelerate the growth of seedlings, and improve the stress resistance and disease resistance of see...

Claims

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Application Information

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IPC IPC(8): C12N1/14C09K17/14C12R1/80C09K101/00
Inventor 吴小芹魏伟叶建仁乔欢
Owner NANJING FORESTRY UNIV
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