Method for inducing embryonal-suspensor mass (ESM) regeneration plant from immature seed of pinus massoniana

Abstract

The invention relates to a method for inducing an embryonal-suspensor mass (ESM) regeneration plant from an immature seed of pinus massoniana. By using the characteristic of continuous schizogenesis capability of the ESM in the immature seed of the pinus massoniana, solid culture and liquid culture are performed alternatively and ESM forms a completely-grown embryo with a normal structure after a maintaining stage, a multiplication stage, an embryo pre-mature stage and a maturation culture stage, so that a pinus massoniana plant is obtained by germinating and culturing the embryo. Due to the adoption of the method, a large number of regeneration plants of a pinus massoniana embryo of which the hereditary basis is accordant can be obtained and new technology with a short period and high occurring efficiency is provided for the storage of pinus massoniana rare generic resource and the mass production of excellent-gene nursery-grown plants.

Description

1. Technical field

[0001] The invention belongs to the technical field of seedling propagation in forestry, and in particular relates to a method for inducing plant regeneration from immature seeds of immature seeds of Pinus massoniana (Embryonal Suspensor Mass, ESM for short). 2. Background technology

[0002] Masson pine (Pinus massoniana) is one of the main timber tree species in southern my country, with a wide distribution and a large stock volume. Because of its strong adaptability, fast growth, wide range of uses, easy reforestation and low cost, it has played a great role in greening barren hills and improving the ecological environment. Compared with commercial Chinese fir and northern red pine, Masson pine surpasses Chinese fir and red pine in terms of bending strength, flexural modulus, pressure along the grain, and end surface hardness; and its wood cellulose content is higher, and its wood is widely used Used in construction, panel, furniture, plywood, paper an...

Examples

Embodiment Construction

[0163] The test materials were immature embryos of excellent trees of Pine masson, collected from the seed orchards of Pine masson clones grafted in Nanning, Guangxi and Nanping, Fujian, respectively, and repeated for 3 years. The cones are collected every 10 days from the end of May to the end of September every year. The seed development stages corresponding to each seed collection time point were the developmental stages from immature embryo to mature embryo. After the cones were collected, they were placed in a pre-frozen ice box on site and wrapped with moist gauze to prevent water loss; then they were quickly transported back to the laboratory and stored in a 4°C refrigerator for later use. Pretreatment at 4°C refrigerator for at least one week is conducive to the efficient induction of ESM. Before inoculation, take the spare cones out of the refrigerator, soak and clean them with detergent-containing lotion, and then rinse them with running water for 2 hours. Take out...

1. Technical field

[0001] The invention belongs to the technical field of seedling propagation in forestry, and in particular relates to a method for inducing plant regeneration from immature seeds of immature seeds of Pinus massoniana (Embryonal Suspensor Mass, ESM for short). 2. Background technology

[0002] Masson pine (Pinus massoniana) is one of the main timber tree species in southern my country, with a wide distribution and a large stock volume. Because of its strong adaptability, fast growth, wide range of uses, easy reforestation and low cost, it has played a great role in greening barren hills and improving the ecological environment. Compared with commercial Chinese fir and northern red pine, Masson pine surpasses Chinese fir and red pine in terms of bending strength, flexural modulus, pressure along the grain, and end surface hardness; and its wood cellulose content is higher, and its wood is widely used Used in construction, panel, furniture, plywood, paper an...

Examples

Embodiment Construction

[0163] The test materials were immature embryos of excellent trees of Pine masson, collected from the seed orchards of Pine masson clones grafted in Nanning, Guangxi and Nanping, Fujian, respectively, and repeated for 3 years. The cones are collected every 10 days from the end of May to the end of September every year. The seed development stages corresponding to each seed collection time point were the developmental stages from immature embryo to mature embryo. After the cones were collected, they were placed in a pre-frozen ice box on site and wrapped with moist gauze to prevent water loss; then they were quickly transported back to the laboratory and stored in a 4°C refrigerator for later use. Pretreatment at 4°C refrigerator for at least one week is conducive to the efficient induction of ESM. Before inoculation, take the spare cones out of the refrigerator, soak and clean them with detergent-containing lotion, and then rinse them with running water for 2 hours. Take out...

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