Method for storing and unfreezing pinus massoniana pollen

A masson pine and pollen technology is applied in the field of masson pine pollen storage and thawing, can solve problems such as the lack of flowering of male and female flowers, and achieves the effects of simple equipment, easy operation and wide applicability

Active Publication Date: 2011-08-10
GUANGXI FORESTRY RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved in the present invention is to provide a method for storing and thawing pine pollen, so that the lifespan of pine pollen is prolonged and the pollen util

Method used

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  • Method for storing and unfreezing pinus massoniana pollen
  • Method for storing and unfreezing pinus massoniana pollen
  • Method for storing and unfreezing pinus massoniana pollen

Examples

Experimental program
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Effect test

Embodiment 1

[0012] like figure 1 Shown, the production process that applies the present invention to process and use masson pine pollen mainly comprises:

[0013] Pollen collection and drying: At the end of February and the beginning of March every year, collect the male flower branches of Masson pine, and bring them back with homemade bags made of sulfuric acid paper. In order to prevent pollen pollution in the seed garden, all flower branches need to be washed with water, and the washed flower branches Put them in different isolation areas for hydroponics. When the flower buds are ready to open, cut them off and put them in a cowhide envelope to seal and then dry. The drying condition is 28°C and constant temperature for 5 hours; the pollen is scattered, and the corner of the envelope is pierced with a small hole with a needle. Collect the pollen into a brown bottle filled with silica gel and seal it with sealant. Wipe off the pollen scattered outside the bottle with 75% alcohol; store...

Embodiment 2

[0022] Collection and drying of pollen: dry at a constant temperature of 25°C for 10 hours, and the moisture content of pollen is 6%.

[0023] Low temperature storage of pollen: low temperature storage at ~10°C.

[0024] Thawing of stored pollen: Refrigerator at 4°C for 21 hours, room temperature for 8 hours, then open the sealant and place for 10 hours.

[0025] Others are basically the same as in Example 1.

Embodiment 3

[0027] Collection and drying of pollen: dry at a constant temperature of 26°C for 7 hours, and the moisture content of pollen is 5%.

[0028] Low-temperature storage of pollen: low-temperature storage at -15°C.

[0029] Thawing of stored pollen: refrigerated at 6°C for 23h, at room temperature for 10h, then open the sealant and place for 9h.

[0030] Others are basically the same as in Example 1.

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Abstract

The invention discloses a method for storing and unfreezing pinus massoniana pollen. The method comprises the following steps of: storing, namely performing water culture on pinus massoniana male flowers with branches and leaves, picking off buds when the buds is to blossom, placing the buds into a kraft paper envelope, drying at constant temperature, dispersing pollen, pinking one corner of the envelope by using a needle, collecting the pollen into a brown bottle with silica gel, sealing the opening with a sealant, storing the brown bottle at normal temperature and in shade, exchanging the silica gel timely when color of the silica gel is changed, and sealing the bottle when the color of the silica gel is not changed and storing the bottle at a low temperature; and unfreezing, namely taking out the brown bottle and placing the brown bottle into a refrigerating chamber of a refrigerator for 20 to 24 hours, taking out the brown bottle and placing the brown bottle at a normal-temperature environment for 8 to 12 hours, opening the sealant and placing the brown bottle for 8 to 12 hours until the color of the silica gel is changed, or opening the opening if the color of the silica gel is not changed, adhering filter paper absorbed with water to the opening, and covering the opening by using a preservative film and sealing the opening by using a rubber band until the color of the silica gel is changed. By the method, life of the pinus massoniana pollen is prolonged; the problems of cross breeding (seed production) of pine tree seeds and flowering asynchronism of artificially auxiliary pollination in a seed orchard are solved; and utilization ratio of the pollen is increased.

Description

technical field [0001] The invention relates to the storage of pollen, in particular to the storage of masson pine pollen and its thawing method. Background technique [0002] Masson pine (Pinus massoniana) is an important timber and resin-producing tree species in southern my country. It has high economic value and is widely used. In addition, its pollen, needles and bark can be used as medicine; pine nuts contain 30% oil, which can be used to make paints, soaps and lubricating oils; Poria cocos. [0003] Guangxi is the main producing area of ​​masson pine in my country, with the best genetic resources and the highest production potential in the country. In the 1980s, Guangxi built a 200-hectare primary seed orchard of Masson pine with growth as the improvement goal, which played an important role in promoting the process of improving the quality of Masson pine. However, due to the fact that there are as many as 464 clones in the seed orchard, the male and female floweri...

Claims

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Application Information

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IPC IPC(8): A01N3/00A01H1/02
Inventor 谭健晖舒文波杨章旗
Owner GUANGXI FORESTRY RES INST
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