Twig and short shoot cutting seedling raising method for pinus massoniana tissue culture seedlings
A technique for raising seedlings by cuttings and Pinus massoniana, applied in the field of plant reproduction, can solve the problems of unstable rooting effect, unsatisfactory rooting rate, low reproduction coefficient of cuttings, etc., achieves extremely easy rooting by cuttings, overcomes the long and obvious subculture cycle of tissue culture, etc. progressive effect
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Embodiment 1
[0029] The invention discloses a method for raising seedlings of masson pine tissue-cultured seedlings with short branches and short spikes. A 70-hole seedling tray with a hole size of 6 cm x 8 cm and serrated holes on the side of the hole is selected as a container for cutting seedlings. Mix the fermented pine bark, coconut bran, yellow heart soil, and pumice in a volume ratio=2:2:4:2 to obtain a seedling-raising substrate, put the seedling-raising substrate into a non-woven fabric seedling-raising bag with a size of 8cm×9cm, and then place the In the seedling-raising tray, then drench the installed seedling-raising matrix with water, and use a mixture of volume concentration 0.1% carbendazim + volume concentration 0.5% potassium permanganate to sterilize the seedling-raising matrix for later use. When cutting, the seedling-raising matrix in the seedling-raising bag The water content is controlled at 65%.
[0030] Select the newly pumped 3cm long, more than 4mm in diameter, i...
Embodiment 2
[0034] The invention discloses a method for raising seedlings of masson pine tissue-cultured seedlings with short branches and short spikes. A 70-hole seedling tray with a hole size of 6 cm x 8 cm and serrated holes on the side of the hole is selected as a container for cutting seedlings. Mix the fermented pine bark, coconut bran, yellow heart soil, and pumice in a volume ratio=2:2:4:2 to obtain a seedling-raising substrate, put the seedling-raising substrate into a non-woven fabric seedling-raising bag with a size of 8cm×9cm, and then place the In the seedling-raising tray, then drench the installed seedling-raising substrate with water, and use a mixture of volume concentration 0.3% carbendazim + volume concentration 0.5% potassium permanganate to sterilize the seedling-raising substrate for later use. When cutting, the seedling-raising substrate in the seedling-raising bag The water content is controlled at 75%.
[0035]Select the newly pumped 4cm long, more than 4mm in dia...
Embodiment 3
[0039] The invention discloses a method for raising seedlings of masson pine tissue-cultured seedlings with short branches and short spikes. A 70-hole seedling tray with a hole size of 6 cm x 8 cm and serrated holes on the side of the hole is selected as a container for cutting seedlings. Mix the fermented pine bark, coconut bran, yellow heart soil, and pumice in a volume ratio=2:2:4:2 to obtain a seedling-raising substrate, put the seedling-raising substrate into a non-woven fabric seedling-raising bag with a size of 8cm×9cm, and then place the In the seedling-raising tray, then drench the installed seedling-raising substrate with water, and use a mixture of volume concentration 0.2% carbendazim + volume concentration 0.5% potassium permanganate to sterilize the seedling-raising substrate for later use. When cutting, the seedling-raising substrate in the seedling-raising bag The water content is controlled at 70%.
[0040] Select the newly pumped 3cm long, more than 4mm in di...
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