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Method for Seedling Cultivation of Pine Masson Tissue Cultured Seedlings by Cutting Short Branches and Short Spikes

A technique for raising seedlings by cuttings and Pinus massoniana, applied in the field of plant reproduction, can solve the problems of unstable rooting effect, low reproduction coefficient of cuttings, unsatisfactory rooting rate, etc., achieves easy rooting by cuttings, overcomes long tissue culture subgeneration cycle, improves Yield and effect of cuttings

Inactive Publication Date: 2018-05-08
GUANGXI FORESTRY RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The invention overcomes the technical problems of low cutting propagation coefficient, unsatisfactory rooting rate and unstable rooting effect in the existing technology of masson pine cutting seedling raising, and provides a masson pine group with high yield of qualified cuttings, high survival rate of cutting rooting and remarkable effect. The method of cultivating young shoots and short panicle cuttings to raise seedlings

Method used

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  • Method for Seedling Cultivation of Pine Masson Tissue Cultured Seedlings by Cutting Short Branches and Short Spikes
  • Method for Seedling Cultivation of Pine Masson Tissue Cultured Seedlings by Cutting Short Branches and Short Spikes
  • Method for Seedling Cultivation of Pine Masson Tissue Cultured Seedlings by Cutting Short Branches and Short Spikes

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Effect test

Embodiment 1

[0029] A method for cutting seedlings of masson pine tissue culture seedlings with short ears and twigs. A 70-hole seedling tray with a hole size of 6 cm×8 cm and a serrated hole on the side of the hole is selected as a cutting seedling container. Mix the fermented pine bark, coconut bran, yellow heart soil, and pumice at a volume ratio of 2:2:4:2 to obtain a seedling substrate. Put the seedling substrate into a non-woven seedling bag with a size of 8 cm×9 cm. Place it in the seedling tray, and then pour the filled seedling substrate with water. Use a mixture of 0.1% carbendazim + 0.5% potassium permanganate to sterilize the seedling substrate for use. When cutting, the seedlings are grown in the seedling bag The water content of the matrix is ​​controlled at 65%.

[0030] Select the soft and vigorous young masson pine twigs with a length of 3 cm and a diameter of 4 mm or more that are newly ligninized, and the top needles are clustered as cuttings; the cuttings collected are qui...

Embodiment 2

[0034] A method for cutting seedlings of masson pine tissue culture seedlings with short ears and twigs. A 70-hole seedling tray with a hole size of 6 cm×8 cm and a serrated hole on the side of the hole is selected as a cutting seedling container. Mix the fermented pine bark, coconut bran, yellow heart soil, and pumice at a volume ratio of 2:2:4:2 to obtain a seedling substrate. Put the seedling substrate into a non-woven seedling bag with a size of 8 cm×9 cm. Place it in the seedling tray, and then pour the filled seedling substrate with water. Use a mixture of 0.3% carbendazim + 0.5% potassium permanganate to sterilize the seedling substrate for later use. When cutting, seedlings are grown in the seedling bag The water content of the substrate is controlled at 75%.

[0035] Select the newly harvested 4 cm long, 4 mm diameter or more, initially lignified, soft and vigorous young masson pine shoots with clustered needles at the top as cuttings; quickly immerse the collected cutti...

Embodiment 3

[0039] A method for cutting seedlings of masson pine tissue culture seedlings with short ears and twigs. A 70-hole seedling tray with a hole size of 6 cm×8 cm and a serrated hole on the side of the hole is selected as a cutting seedling container. Mix the fermented pine bark, coconut bran, yellow heart soil, and pumice at a volume ratio of 2:2:4:2 to obtain a seedling substrate. Put the seedling substrate into a non-woven seedling bag with a size of 8 cm×9 cm. Place it in the seedling tray, then pour the filled seedling substrate with water, and use a mixture of 0.2% carbendazim + 0.5% potassium permanganate to disinfect the seedling substrate for use. When cutting, the seedlings are grown in the seedling bag The water content of the substrate is controlled at 70%.

[0040] Select the soft and vigorous young masson pine twigs with a length of 3 cm and a diameter of 4 mm or more that are newly ligninized, and the top needles are clustered as cuttings; the cuttings collected are qu...

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Abstract

The invention discloses a twig and short shoot cutting seedling raising method for pinus massoniana tissue culture seedlings. The method includes the working procedures of selecting a container, preparing a matrix, collecting cutting shoots, treating the cutting shoots, conducting cuttage and managing a nursery; the suitable seedling raising container is selected, after the matrix is prepared according to a certain ratio and disinfected through a disinfecting method, juvenile-form twigs are collected to serve as the cutting shoots to be eluted and disinfected, after the cutting shoots are treated with a root growth promoting agent, cuttage is directly carried out on the cutting shoots, control over light, temperature, water and fertilizer conditions is enhanced in the period, the cutting shoots take roots about a month later, the rooting rate reaches 98% or above, and the survival rate reaches 97% or above. The pinus massoniana tissue culture seedlings serve as cutting shoot stock plants, the problems that the tissue culture subculture period is long, seedling raising cost is high are solved, the propagation rate is large, the number of qualified juvenile-form twigs for cottage is large, cutting rooting is easy, the effect is remarkable, and the seedling raising method has good economic benefits, social benefits and ecological benefits.

Description

Technical field [0001] The invention belongs to the technical field of plant propagation, and relates to tissue culture seedling raising technology, in particular to a method for raising young shoots and short ear cuttings of masson pine tissue culture seedlings. Background technique [0002] Masson pine ( Pinus massoniana Lamb.) is the main afforestation tree species in southern my country. Due to its dual-use characteristics, it has high comprehensive utilization value and broad promotion prospects. In recent years, with the intensification of contradictions between people's increasing living needs, the shortage of wood resources and the gradual exhaustion of non-renewable resources such as oil, the sustainable development of ecology, economy and society has attracted more and more attention from the society. Based on my country's ecological safety, resource security and social security, Masson pine is my country's main industrial timber species and biomass energy tree species,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G2/10A01G17/00A01G9/029A01G9/28A01N59/26A01N59/00A01N37/10A01N43/38A01N43/647A01N43/90A01N43/78A01N43/40A01P21/00
CPCA01G2/00A01G9/029A01G9/0299A01G17/00A01G24/00A01G24/23A01N37/10A01N43/38A01N43/40A01N43/647A01N43/78A01N43/90A01N59/00A01N59/26A01N2300/00
Inventor 姚瑞玲王胤苏烜
Owner GUANGXI FORESTRY RES INST
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