The invention discloses a multi-step vertical shearing testing method in a soft soil chamber, which is characterized in that 1) N samples of a same soil sample are arranged in the shearing box of the soft soil chamber; 2) first-step normal load Sigma1 is acted on the N samples simultaneously; under the normal load, a shearing force vertical to the normal load Sigma is acted on the sample; furthermore, respective shearing stress peak values, Tau<1-1>, Tau<1-2>, Tau<1-3>, ellipsis, Tau<1-N>, are respectively recorded; subsequently, all normal loads are unloaded and quickly retrieved; 3) the step 2) is repeated; the second-step normal load Sigma2, the third-step normal load Sigma3,..., the M-step normal load Sigma<M> are acted on the N samples simultaneously, thus obtaining M groups of shearing stress peak values, Tau<M-1>, Tau<M-2>, ellipsis, Tau<M-N>; 4) to the M groups of normal load Sigma<M> and the shearing stress peak values Tau<M-1>, Tau<M-2>, Tau<M-3>, and Tau<M-N> of each sample, according to a formula that Tau<M-N> is equal to c<N>+Sigma<M>tanPhi<N>, the anti-shearing strength parameters, c<N> and Phi<N> of the sample are obtained; and 5) the average value of the anti-shearing strength parameters of the N samples are used as the anti-shearing strength parameter value of the group of soil samples. The method has the effects of saving testing sample resource and has concentrative and exact testing results.