Organ vitrification freezing and preserving fluid

Abstract

The invention relates to the field of medical organ preservation and more particularly discloses a vitrification freezing and preserving fluid for preserving organs for a long term. The organ vitrification freezing and preserving fluid is characterized by comprising dimethyl sulfoxide, acetamide, glycerol, ethylene glycol, propylene glycol, cane sugar, polyethylene glycol and the like, wherein the concentrations of the dimethyl sulfoxide, the acetamide, the glycerol, the ethylene glycol, the propylene glycol and the cane sugar are 0.1-6 mol / L; the concentration of the polyethylene glycol is 1-20 mmol / L; and the pH value is in a range from 5.0 to 8.0. By implementing the vitrification preserving fluid for preserving organs, provided by the invention, the preserving fluid is in a glassy state rather than forming ice crystal in the cooling and freezing process to protect the tissue morphology and structure completeness, completely suppresses energy metabolism of tissue cells under a freezing condition and recovers biological functions of organs after anabiosis. The organ vitrification freezing and preserving fluid, disclosed by the invention, can be used for freezing and preserving kidney and also can be further widely applied to freezing and preserving of other organs for a long term for scientific research and clinic application.

Description

technical field [0001] The kidney is an organ with complex functions in the human body, and it can only be preserved in vitro for a maximum of 50 hours at 4°C perfusion. To preserve the kidney for a long time or even permanently, the storage temperature must be further lowered, the metabolic rate of the tissue cells must be suppressed, and even the cell metabolism must be completely stopped. If the kidneys can be preserved for a long time under suitable freezing conditions, and the cryopreserved donor kidneys can be "resuscitated" by appropriate means and then transplanted when needed, it will open up a new prospect for clinical transplantation. [0002] The cryopreservation method is considered to be an ideal way to achieve long-term preservation of organs. The key to the problem is that the organs are large in size, and it is difficult to overcome the formation of intracellular and intracellular ice crystals during cooling and rewarming. Therefore, the problem of cryopreser...

AI Technical Summary

Problems solved by technology

Although scholars at home and abroad start to prolong the preservation time of kidneys by improving the level of preservation solution and preservation tools, or improve the tissue matching work within the limited preservation time, so as to improve the reasonable utilization rate of donor kidneys and the long-term survival rate after transplantation, However, it still cannot meet the clinical needs

In addition, in the absence of donor organs in China, certain organ resources will be wasted due to reasons such as matching, patient conditions, and regions.