Organ cryopreservation fluid

Abstract

The invention relates to the medical organ preservation field, and in particular discloses an organ cryopreservation fluid for preserving the organ for a long time. The organ cryopreservation fluid is characterized by comprising the ingredients such as dimethyl sulfoxide, acetamide, glucose, glycerol, polyethylene glycol and the like; the concentrations of the dimethyl sulfoxide, the acetamide, the glucose and the glycerol are 0.2 to 5 mol/L; and the concentration of the polyethylene glycol is 1 to 20 mol/L. The cryopreservation fluid for preserving the organs in the invention can protect shape and structure integrity of tissue cells under the cryopreservation under the condition that energy metabolism is fully inhibited, and recover biological function after proper condition anabiosis; and the cryopreservation fluid can be used for cryopreservation for kidneys, and also can be applied to cryopreservation for other organs so that the organs can be preserved for a long time in clinical practices.

Description

technical field [0001] The cryopreservation method is considered to be an ideal way to achieve long-term preservation of organs. The key to the problem is that the organs are large in size, and it is difficult to overcome the formation of intracellular and intracellular ice crystals during cooling and rewarming. Therefore, the problem of cryopreservation of large-volume organs has not yet been solved. The kidney is an organ with complex functions in the human body, and it can only be preserved in vitro for a maximum of 50 hours at 4°C perfusion. If the donated kidney can be preserved for a long time under suitable cryogenic conditions, it will open up a new prospect for clinical transplantation. Background technique [0002] Recently, cryopreservation of cells, sperm, embryos and even ovarian tissue has been successfully achieved, but the cryopreservation of complex organs such as the kidney is still a problem that has not yet been broken through. In today's world, the num...

AI Technical Summary

Problems solved by technology

At present, the donor kidneys used in clinical kidney transplantation are all stored above 0°C. Although this method is relatively mature and simple, the storage time is limited. Scholars at home and abroad have started to prolong the storage time of kidneys by improving the level of storage solution and storage tools. Or improve the tissue matching work within the limited storage time to improve the reasonable utilization rate of the donor kidney and the long-term survival rate after transplantation, but it still cannot meet the clinical needs

In addition, in the absence of donor organs in China, certain organ resources will be wasted due to reasons such as matching, patient conditions, and regions.