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Marine bdellovibrio long-stem preservation method

A technology for long-term preservation of Bdellovibrio, which is applied in the direction of bacteria and microorganism preservation. It can solve the problems of high viable bacteria content, high preservation cost, and difficulty in long-term preservation, and achieve cost-saving, simple preservation methods and good preservation effects.

Active Publication Date: 2019-09-20
GUANGDONG OCEAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, Bdellovibrio, including marine Bdellovibrio, is usually obligately parasitic, so it is difficult to carry out simple and effective long-term storage, especially at room temperature, it is difficult to maintain a high content of viable bacteria, which seriously restricts the development of Bdellovibrio. The application of Vibrio; on the other hand, most of the existing Bdellovibrio preservation studies focus on terrestrial Bdellovibrio, and basically adopt the method of low temperature preservation for preservation, the effect of room temperature preservation is not very ideal, and the preservation cost is high. The specific preservation of Bdellovibrio is rarely reported

Method used

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  • Marine bdellovibrio long-stem preservation method
  • Marine bdellovibrio long-stem preservation method
  • Marine bdellovibrio long-stem preservation method

Examples

Experimental program
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Effect test

Embodiment 1

[0024] In this example, the strains of the Halobacteriovorax genus were selected as the preserved marine Bdellovibrio species, and the Chinese translation of the Halobacteriovorax genus was called the halophilic phage Vibrio genus, and Vibrio parahaemolyticus was selected as the host bacteria for double-layer plate counting of the marine Bdellovibrio species.

[0025] Prepare sterile seawater, the salinity of the sterile seawater is 25-35, and the pH is 7-9, and all are filtered and sterilized. The salinity of the sterile seawater in the present embodiment is 29.8-30.2, and the pH is 7.8-8.2.

[0026] Configure SWYE medium, the configuration process is as follows: take NaCl 24g, MgCl 7H 2 O 7g, KCl 0.7g, MgCl 6H 2 O 5.3g, 1L of distilled water to prepare a four-salt solution with a pH of 7.4-7.8, then take 10g of peptone and 3g of yeast extract, dilute to 1L with four-salt solution, adjust the pH to 7.2-7.4, and autoclave at 121°C for 20min It is used as the culture of host ...

Embodiment 2

[0039] In this example, bacteria of the genus Halobacteriovorax were selected as the preserved marine Bdellovibrio species, and Vibrio parahaemolyticus was selected as the host bacteria for double-layer plate counting of marine Bdellovibrio.

[0040] Prepare sterile seawater, the salinity of the sterile seawater is 25-35, and the pH is 7-9, and all are filtered and sterilized. The salinity of the sterile seawater in the present embodiment is 29.8-30.2, and the pH is 7.8-8.2.

[0041] Configure SWYE medium, the configuration process is as follows: take NaCl 24g, MgCl 7H2O 7g, KCl 0.7g, MgCl 6H2O 5.3g, distilled water 1L to prepare a tetra-salt solution with a pH of 7.4-7.8, then take peptone 10g and yeast extract 3g , dilute to 1 L with tetra-salt solution, adjust the pH to 7.2-7.4, autoclave at 121° C. for 20 minutes, and use it for the cultivation of host bacteria in this example.

[0042] Prepare seawater double-layer agar medium, the bottom layer of the seawater double-lay...

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Abstract

The invention relates to the technical field of strain preservation and discloses a marine bdellovibrio long-stem preservation method. The method includes host bacterium seawater suspension preparation, marine bdellovibrio seawater suspension preparation and strain preservation. A traditional concept of low-temperature long-term preservation of bdellovibrio is broken through, long-term preservation of bdellovibrio under a normal-temperature condition is realized, the preservation method is simple and great in preservation effect, and long-term preservation cost is saved.

Description

technical field [0001] The invention relates to the technical field of strain preservation, in particular to a long-term preservation method for marine Bdellovibrio. Background technique [0002] Bdellovibrio-like organisms, or Bdellovibrio for short, are small bacteria that can lyse other bacteria. The unique predation characteristics of Bdellovibrio can be used as a "live antibiotic", which has significant application value in replacing antibiotics to control harmful bacteria, and has been used in production practice. [0003] Marine Bdellovibrio can lyse a variety of Gram-negative bacteria, especially Vibrio, which has obvious advantages in controlling Vibrio and the diseases caused by it. However, Bdellovibrio, including marine Bdellovibrio, is usually obligately parasitic, so it is difficult to carry out simple and effective long-term storage, especially at room temperature, it is difficult to maintain a high content of viable bacteria, which seriously restricts the de...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/04
CPCC12N1/20C12N1/04
Inventor 薛明温崇庆刘美华
Owner GUANGDONG OCEAN UNIVERSITY
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