A kind of preservation method of liquid bacterial agent for sewage treatment
A liquid bacterial agent and preservation method technology, which is applied to the preservation of microorganisms, etc., can solve the problems of inability to store bacterial agents on a large scale, and achieve good treatment effects, reduced storage costs, and high bacterial activity.
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Embodiment 1
[0019] Degradation of oily wastewater before and after storage by commercially available oily wastewater degrading bacteria (the OBT deoiling bacteria from Bioform Biotechnology Co., Ltd. was used in this example).
[0020] The oily wastewater degrading bacteria were expanded and cultivated by a fermenter to prepare 10L of oily wastewater degrading bacteria liquid (bacteria quantity: 2.5×10 9 cfu / ml, using the plate counting method, the same below), and stored in two batches in 5L square small-mouth plastic buckets. Using the preservation technology described in the present invention, the preservation steps of the degrading bacterial liquid of oily wastewater are as follows: adjust the pH value of the bacterial liquid to 6 with 0.05mol / L phosphate buffer solution, feed nitrogen into the plastic bucket for aeration for 30 minutes, press 0.5‰ and 2 Ascorbic acid and potassium sorbate were added at a ratio of ‰ (m / v), sealed with liquid paraffin and stored at room temperature.
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Embodiment 2
[0024] Comparison of the phenol-degrading activity of commercially available (OBT lysing bacteria from Biwofeng Biotechnology Co., Ltd.) before and after storage of phenol-degrading bacteria.
[0025] The phenol-degrading bacteria is expanded and cultivated by a fermenter, and 10L of bacterial liquid is prepared (bacteria quantity: 3.2×10 9 cfu / ml), and stored in two batches in 5L square small-mouth plastic buckets. Using the preservation technique described in the present invention, the preservation steps of the phenol-degrading bacterial liquid are as follows: adjust the pH of the bacterial liquid to 6.2 with 0.07mol / L citrate buffer solution, feed nitrogen into the plastic bucket for 20min, and press the ratio of 0.8‰ and 3‰ respectively Add ascorbic acid and potassium sorbate, cover with liquid paraffin and keep sealed at room temperature.
[0026] After preservation by the above method, a batch of preserved bacterial liquids were opened for counting when they were preser...
Embodiment 3
[0029] Degradation of ammonia nitrogen by commercially available nitrifying bacteria (the BZT nitrifying bacteria of BIOFORM Biotechnology Co., Ltd. was used in this example) before and after storage.
[0030] Mix the nitrifying bacteria into 10L bacterial solution (bacterial quantity 2.6×10 9 cfu / ml), and stored in two batches in 5L square small-mouth plastic buckets. Using the preservation technique described in the present invention, the preservation steps of nitrifying bacteria are as follows: use 0.1mol / L citrate buffer solution to adjust the pH of the bacterial solution to 6.5, feed nitrogen into the plastic bucket for 10 minutes, and add ascorbic acid in proportions of 1‰ and 4‰ respectively and potassium sorbate, covered with liquid paraffin and sealed at room temperature.
[0031] After being preserved by the above method, a batch of bacteria liquids were opened for counting when they were stored for 6 months and 12 months, and compared with newly prepared nitrifying...
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