Preserving fluid and preserving method for maintaining high cell activity of tissue sample
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A technology for tissue samples and tissue cells, which is applied in the field of preservation solution for maintaining high cell activity of tissue samples and preservation solution for cell survival of fresh tissue samples. Capacitance, maintenance of stability, and high cell viability effects
Inactive Publication Date: 2020-07-17
上海伯豪生物技术有限公司
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[0007] None of the preservation methods in the above three invention patent applications can maintain the high cell activity of the tissue and the integrity of the gene information without changing the expression of the tissue cell gene
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Embodiment 1
[0044] Embodiment 1: the preparation of preservation solution A
[0045] In a specific embodiment, the composition of the preservation solution includes potassium hydroxide (5-6 g / L), lactobionic acid (30-40 g / L), sodium dihydrogen phosphate (3-5 g / L), Magnesium sulfate water (0.5-1 g / L), raffinose (15-20 g / L), hydroxyethyl starch (50-60 g / L), adenosine (1-2 g / L), glutathione Peptide (0.8-2 g / L), dissolved in sterile deionized water, using sodium hydroxide and dilute hydrochloric acid to adjust the pH of the solution to 7.2-7.6.
Embodiment 2
[0046] Embodiment 2: the preparation of preservation solution B
[0047] In a specific embodiment, the composition of the preservation solution includes potassium hydroxide (5-6 g / L), lactobionic acid (30-40 g / L), potassium dihydrogen phosphate (3-5 g / L), Magnesium sulfate water (0.6-1.2 g / L), raffinose (15-20 g / L), hydroxyethyl starch (50-60 g / L), adenosine (1-2 g / L), allopurine Alcohol (0.2-0.5 g / L), glutathione (0.5-2 g / L), dissolved in sterilized deionized water, using potassium hydroxide and dilute hydrochloric acid to adjust the pH of the solution to 7.2-7.6.
Embodiment 3
[0048] Embodiment 3: the preparation of preservation solution C
[0049] In a specific embodiment, the composition of the preservation solution includes potassium hydroxide (5-6 g / L), lactobionic acid (30-40 g / L), sodium dihydrogen phosphate (3-5 g / L), Magnesium sulfate water (0.5-1 g / L), raffinose (15-20 g / L), hydroxyethyl starch (50-60 g / L), adenosine (1-2 g / L), allopurinol (0.2-0.5 g / L), glutathione (0.5-2 g / L), dissolved in sterilized deionized water, using sodium hydroxide and dilute hydrochloric acid to adjust the pH of the solution to 7.2-7.6.
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Abstract
The invention provides a preserving fluid and a preserving method for maintaining high cell activity of a tissue sample, wherein the preserving fluid comprises the components: ion buffer solution components, carbohydrate components,, components avoiding the generation of ice crystals in and out solutions of tissue cells at a low temperature of 0-6 DEG C, components providing supplement, antioxidant and anti-apoptosis components for anabolism of the tissue cells; the preserving fluid has good biocompatibility, does not contain toxic or harmful components, and also does not contain components such as animal-derived protein, antibiotics and hormones influencing tissue gene expression. Different from traditional ultralow-temperature cryopreservation (-80 DEG C), the cryopreservation method disclosed by the invention can effectively realize low-temperature storage (0-6 DEG C) of fresh tissue samples of human beings and animals, maintains high cell activity of tissues, maintains tissue morphology, and is used for preservation and transportation of the tissue samples. Meanwhile, the nucleic acid integrity of the tissue and the stability of gene expression can be effectively maintained, the original state of the tissue is maintained, and the method can be used for gene detection, scientific research and other related experiments.
Description
technical field [0001] The present invention relates to the field of biotechnology, in particular to a preservation solution for maintaining the survival of fresh human or animal tissue sample cells without changing the gene expression of the tissue sample; especially relates to a preservation solution for maintaining high cell activity of tissue samples; In addition, the present invention also relates to a preservation method using the preservation solution. Background technique [0002] With the rapid development and popularization of genetic testing technology, how to safely and effectively preserve the collected biological samples has become the key. There is a time and space distance between the collection of fresh tissue samples and the laboratory testing. How to ensure that the tissue samples maintain the activity of cells and the stability of gene expression in this process will determine whether the experimental results are accurate and effective. In particular, si...
Claims
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Application Information
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