Simulation three-dimensional incubator and culture method for mouth mucosa epithelial cells and applications of incubator and culture method

A technology for oral mucosa and epithelial cells, applied in the field of cell culture, which can solve the problems of three-dimensional culture equipment and culture methods for oral mucosa epithelial cells that have not been seen

Inactive Publication Date: 2013-08-21
GUANGDONG UNIV OF TECH
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  • Abstract
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Problems solved by technology

[0004] At present, three-dimensional culture technology has been applied to in vitro culture models of various tumor cells and stem cells, but there are no reports on three-dimensional culture devices and culture methods for oral mucosal epithelial cells

Method used

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  • Simulation three-dimensional incubator and culture method for mouth mucosa epithelial cells and applications of incubator and culture method
  • Simulation three-dimensional incubator and culture method for mouth mucosa epithelial cells and applications of incubator and culture method
  • Simulation three-dimensional incubator and culture method for mouth mucosa epithelial cells and applications of incubator and culture method

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Embodiment Construction

[0022] The present invention is further explained below in conjunction with the examples, but the examples do not limit the present invention in any form.

[0023] like Figure 1 to Figure 3 As shown, a simulated three-dimensional culture device for oral mucosal epithelial cells, which includes a single or multiple basic structural units, the basic structural unit is composed of a tightly nested inner container 1 and an outer container 2, and the inner container 1 and the outer container The container 2 is made of organic materials used for cell culture; the bottom of the inner container 1 is made of a permeable support membrane 3 and has a certain gap with the bottom of the outer container 2 . The device can be composed of a single inner container and outer container or a series of inner containers and outer containers arranged vertically and horizontally.

[0024] In a further preferred solution, the inner container 1 and the outer container 2 are cylindrical.

[0025] In...

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Abstract

The invention discloses a simulation three-dimensional incubator and a culture method for mouth mucosa epithelial cells and applications of the incubator and the culture method. The incubator comprises a single or a plurality of basic structural units, wherein each basic structural unit consists of an inner container and an outer container which are tightly nested with each other, and are prepared from an organic material for cell culture; and the bottom of the inner container consists of a permeable supporting film, and a certain gap is formed between the bottom of the inner container and the bottom of the outer container. The incubator takes the factors of nutrient supply of the mouth mucosa epithelial cells, blood vessels and blood vessel endothelial cells into account, and the growing environment of the mouth mucosa epithelial cells in the incubator is close to that in a human body, so that the incubator can be used for physiological, pharmacological, toxicological and micro-ecological studies of mouth mucosas, and can also be used for constructing human tissue engineering mouth mucosas and repairing and reconstructing mucosal coloboma. The new technology of the simulation three-dimensional incubator promotes an in vitro culture model of a mouth mucosa to a new height, so that related studies are closer to clinical practices.

Description

technical field [0001] The invention relates to the field of cell culture, in particular to a simulated three-dimensional culture device for oral mucosal epithelial cells, a culture method and an application thereof. Background technique [0002] The in vitro culture of oral mucosal epithelial cells has important biological and clinical significance. It not only provides a good experimental system for the study of oral mucosal differentiation, oral pathophysiology, toxicology and carcinogenesis, but also has great significance for the construction of human tissue engineering oral mucosa and the repair of oral mucosal tissue defects. The repair and reconstruction of mucosal defects such as trachea and urethra have important clinical significance. [0003] Both the development and function of cells depend on the interactions between molecules in the cellular microenvironment. Traditional two-dimensional cell culture is a relatively common method of cell culture, and it is no...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00C12N5/073A61L27/38
Inventor 郑希杜志云艾伦康尼张焜李冬利
Owner GUANGDONG UNIV OF TECH
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