Dielectric material for brain-computer interface, brain spine interface or eye machine interface and application thereof

Abstract

The invention discloses a dielectric material for a brain-computer interface, a brain spine interface or an eye-computer interface and application of the dielectric material. The dielectric material comprises a 3D cell layer, the 3D cell layer is prepared from a membrane matrix and cells; the cells are selected from autologous oral mucosa epithelial cells, allogenic umbilical cord mesenchymal stem cells or allogenic amniotic mesenchymal stem cells; according to the medium material, the biological compatible material is adopted, so that the immune rejection reaction between an implanted chip and human tissues can be reduced, the service life can be prolonged, the contact mode of the implanted chip and brain tissues can be optimized, the stability and integrity of signal acquisition are ensured, and signal abnormity is avoided; meanwhile, the infection risk can be reduced, and formation of colloid scar tissue can be prevented. Wide application prospects are realized.

Description

Technical Field

[0001] The present invention relates to the technical field of brain-computer interface and eye-computer interface media, and in particular to a dielectric material for brain-computer interface, cerebrospinal interface or eye-computer interface and its application. Background Art

[0002] Brain-computer interface (BCI), brain-spinal interface (CSI), and eye-computer interface (OCI) technologies have shown great potential in the fields of medicine, rehabilitation, and human-computer interaction. Existing BCI technologies primarily rely on implantable or non-implantable devices to collect neural signals from the brain or eyes and convert them into computer-readable instructions. However, existing technologies face the following problems: Poor biocompatibility: Existing BCI media often trigger immune rejection reactions, resulting in a short lifespan of the implanted devices, typically only about a year, and requiring frequent replacement. Furthermore, submental chip implantation may trigger local inflammatory reactions, leading to chronic inflammation. Chronic inflammation may promote cell damage and mutations, increasing the risk of gliomas. Immune system activation: As a foreign body, BCI media (chips) may activate the immune system, triggering abnormal immune responses. Long-term immune activation may interfere with the normal function of brain tissue and increase the likelihood of cell malignancy. Mechanical stimulation: Chip implantation may mechanically stimulate surrounding brain tissue, causing cell damage or abnormal proliferation, thereby increasing the risk of tumor development. Unstable signal acquisition: In existing technologies, the signal acquisition area of BCIs is limited, and the interaction between signals and brain tissue is not close enough, resulting in incomplete or unstable signal acquisition. While implantable devices are effective, they carry a high risk of infection: they are prone to infection and may induce neurological disorders such as epilepsy. They can also cause glial scar tissue to form in the brain: When implanted devices come into contact with brain tissue, they can easily trigger glial scar tissue formation, leading to signal abnormalities and further compromising the long-term use of the device.

[0003] In view of this, the present invention is proposed. Summary of the Invention

[0004] The purpose of the present invention is to provide a dielectric material for brain-computer interface, cerebrospinal interface or eye-computer interface and its application. By adopting biocompatible materials, the dielectric material of the present invention can reduce the immune rejection reaction between the implanted chip and human tissue, extend the service life, and optimize the contact mode between the implanted chip and brain tissue, spinal nerves, or eye tissue, ensuring the stability and integrity of signal acquisition, avoiding signal abnormalities, and at the same time reducing the risk of infection and preventing the formation of glial scar tissue.

[0005] In order to achieve the above-mentioned purpose of the present invention, the following technical solutions are adopted:

[0006] A first aspect of the present invention provides a dielectric material for a brain-computer interface, a brain-spinal interface, or an eye-computer interface, the dielectric material comprising a 3D cell layer;

[0007] The 3D cell layer is prepared from a membrane matrix and cells; the cells are selected from autologous oral mucosal epithelial cells, allogeneic umbilical cord mesenchymal stem cells or allogeneic amniotic membrane mesenchymal stem cells.

[0008] Immune cells in allogeneic umbilical cord or amniotic membrane tissue are relatively immature, with immature immune functions, in a quiescent state, or not yet developed. Therefore, as immune-privileged cells, these cells are less likely to trigger immune or stress responses during allogeneic transplantation. Umbilical cord and amniotic membrane are waste medical materials that are readily available and ethically safe.

[0009] Preferably, when the dielectric material is used for a brain-computer interface or a brain-spinal interface, the dielectric material further comprises an isolation membrane adhered to the 3D cell layer.

[0010] Preferably, if the medium material is used for a brain-computer interface or a brain-spinal interface, the cells are autologous oral mucosal epithelial cells, allogeneic umbilical cord mesenchymal stem cells, or allogeneic amniotic membrane mesenchymal stem cells;

[0011] The 3D cell layer is prepared by the following method:

[0012] (a) culturing and passaged the cells to obtain P2 to P4 cells, and then resuspending the cells in DMEM / F12 medium to obtain a cell suspension, wherein the DMEM / F12 medium contains the following components: 10% fetal bovine serum, 1% penicillin / streptomycin, and 510 ng / mL bFGF;

[0013] (b) adding the cell suspension dropwise onto the membrane matrix for culturing to form a cell layer on the membrane matrix, thereby obtaining a membrane matrix with a cell layer attached thereto; forming a three-dimensional cell layer, namely, the 3D cell layer.

[0014] Preferably, in the present invention, by repeating step (c), multiple cell layers can be formed on the membrane matrix to obtain the 3D cell layer.

[0015] Preferably, in step (b), the cell concentration in the cell suspension is (1-10)×10 5 / ml; the amount of cell suspension added is (1~5)×10 4 pieces / cm 2 ; If the medium material is used for brain-computer interface, the culture time is 14 to 21 days; if the medium material is used for brain-spinal interface, the culture time is 5 to 7 days.

[0016] Preferably, if the medium material is used for an eye-machine interface, the cells are allogeneic umbilical cord mesenchymal stem cells or allogeneic amniotic membrane mesenchymal stem cells;

[0017] The 3D cell layer is prepared by the following method:

[0018] (1) culturing and passaged allogeneic umbilical cord mesenchymal stem cells or allogeneic amniotic membrane mesenchymal stem cells to obtain P2 to P4 cells, and then resuspending them in DMEM / F12 low-glucose medium to obtain an allogeneic umbilical cord mesenchymal stem cell suspension or an allogeneic amniotic membrane mesenchymal stem cell suspension;

[0019] (2) adding an allogeneic umbilical cord mesenchymal stem cell suspension or an allogeneic amniotic membrane mesenchymal stem cell suspension onto the membrane matrix for culture to form a cell layer on the membrane matrix, thereby obtaining a membrane matrix with a cell layer attached, forming a three-dimensional cell layer, namely, the 3D cell layer.

[0020] Preferably, in step (2), the cell concentrations of the allogeneic umbilical cord mesenchymal stem cell suspension or the allogeneic amniotic membrane mesenchymal stem cell suspension are (1-10)×10 5 / ml; the amount of dripping is (1~5)×10 4 pieces / cm 2 ; The culture time is 24 to 30 days;

[0021] If the eye-machine interface is located at the cornea, the DMEM / F12 low-glucose culture medium contains the following components: 10% fetal bovine serum, 1% penicillin / streptomycin, 1020 ng / mL EGF, 510 ng / mL KGF, 15 ng / mL TGFβ, 5 μg / mL insulin, 0.4 μg / mL hydrocortisone, and 50 μg / mL ascorbic acid;

[0022] If the eye-machine interface is located at the retina, the DMEM / F12 low-glucose culture medium contains the following components: 2% B27 supplement, 1% non-essential amino acids, 1% L-glutamine, 1% penicillin / streptomycin, 1020ng / mL Activin A, 1020ng / mL BMP4, 50100ng / mL DKK1, 10ng / mL FGF2 and 0.51μM retinoic acid.

[0023] The second aspect of the present invention provides a medium product for brain-computer interface, brain-spinal interface or eye-computer interface, which includes a transparent film, the medium material, and a supporting film for internal identification to prevent deformation of the deformable medium, namely a basement film and a foil film, which are laminated in sequence.

[0024] A third aspect of the present invention provides an application of the above-mentioned dielectric material or the above-mentioned dielectric product in the preparation of a brain-computer interface, a brain-spinal interface or an eye-computer interface.

[0025] A fourth aspect of the present invention provides a brain-computer interface, brain-spinal interface or eye-computer interface, comprising the above-mentioned dielectric material and chip.

[0026] Preferably, the dielectric material wraps the chip.

[0027] Compared with the prior art, the beneficial effects of the present invention include at least:

[0028] By using biocompatible materials, the dielectric material of the present invention can reduce the immune rejection reaction between the implanted chip and human tissue, thereby extending the service life. At the same time, the dielectric material of the present invention has the characteristics of ultra-thinness, softness, and curlability at the nanometer level, and can adapt to the curved surface structure of the brain and eyes, ensuring the stability and integrity of signal acquisition and avoiding signal abnormalities. In addition, the contact surface between the 3D cell layer and the chip adopts a cell contact method and is hydrophilic. The back side is provided with an isolation membrane to prevent foreign matter from forming gliomas and scar tissue with meningeal tissue.

[0029] The dielectric material of the present invention can reduce stimulation of the implanted chip on brain tissue by wrapping the chip before implantation, prevent the formation of glial scar tissue, and avoid the occurrence of signal abnormalities and epilepsy. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] To more clearly illustrate the specific embodiments of the present invention or the technical solutions in the prior art, the following briefly describes the drawings required for the specific embodiments or the description of the prior art. Similar elements or parts are generally identified by similar reference numerals throughout the drawings. Elements or parts in the drawings are not necessarily drawn to scale.

[0031] Figure 1 Schematic diagram of the structure of a medium product for preparing a brain-computer interface, a brain-spinal interface, or an eye-computer interface in an embodiment of the present invention;

[0032] Figure 2 This is a schematic diagram of the result of wrapping a hard chip with a dielectric material in an embodiment of the present invention;

[0033] Figure 3 Schematic diagram of covering the skull defect with different dielectric materials in an experimental example of the present invention;

[0034] Figure 4 Schematic diagram of the cornea covered with different dielectric materials in an experimental example of the present invention;

[0035] Figure 5 This is a staining image of the 3D cell layer of Example 1 in the experimental example of the present invention;

[0036] Figure 6 This is a staining diagram of the membrane matrix in the experimental example of the present invention;

[0037] Figure 7 This is a staining image of the 3D cell layer in Example 4 of the experimental example of the present invention. DETAILED DESCRIPTION

[0038] The following embodiments of the technical solution of the present invention are described in detail in conjunction with the embodiments. The following embodiments are only used to more clearly illustrate the technical solution of the present invention and are therefore only used as examples and cannot be used to limit the scope of protection of the present invention.

[0039] It should be noted that, unless otherwise specified, the technical or scientific terms used in this application should have the common meanings understood by those skilled in the art to which the present invention belongs.

[0040] An embodiment of the present invention provides a dielectric material for a brain-computer interface, a brain-spinal interface, or an eye-computer interface, wherein the dielectric material includes a 3D cell layer;

[0041] The 3D cell layer is prepared from a membrane matrix and cells; the cells are selected from autologous oral mucosal epithelial cells, allogeneic umbilical cord mesenchymal stem cells or allogeneic amniotic membrane mesenchymal stem cells.

[0042] By using biocompatible materials, the dielectric material of the present invention can reduce the immune rejection reaction between the implanted chip and human tissue, thereby extending the service life. At the same time, the dielectric material of the present invention has the characteristics of ultra-thinness, softness, and curlability at the nanometer level, and can adapt to the curved surface structure of the brain and eyes, ensuring the stability and integrity of signal acquisition and avoiding signal anomalies.

[0043] The dielectric material of the present invention can reduce stimulation of the implanted chip on brain tissue by wrapping the chip before implantation, prevent the formation of glial scar tissue, and avoid the occurrence of signal abnormalities and epilepsy.

[0044] In one embodiment, when the dielectric material is used for a brain-computer interface or a brain-spinal interface, the dielectric material also includes an isolation membrane adhered to the 3D cell layer; through the setting of the isolation membrane, the 3D cell layer and the chip contact surface adopt a cell contact method and are hydrophilic, and the back side is prevented from forming glial scar tissue through the setting of the isolation membrane.

[0045] In addition, when the dielectric material is used in the eye-machine interface, no isolation film is required, mainly because the retinal nerve directly adheres to the retinal 3D cell layer and is suitable for repairing defects and retinal diseases. The optic nerve is an important channel connecting the retina and the brain, transmitting visual information.

[0046] In one embodiment, the cell sheet matrix is Membrane matrix or Cell The membrane matrix can be purchased commercially.

[0047] In one embodiment, if the medium material is used for a brain-computer interface or a brain-spinal interface, the cells are autologous oral mucosal epithelial cells, allogeneic umbilical cord mesenchymal stem cells, or allogeneic amniotic membrane mesenchymal stem cells;

[0048] The 3D cell layer is prepared by the following method:

[0049] (a) culturing and passaged the cells to obtain P2 to P4 cells, and then resuspending the cells in DMEM / F12 medium to obtain a cell suspension, wherein the DMEM / F12 medium contains the following components: 10% fetal bovine serum, 1% penicillin / streptomycin, and 510 ng / mL bFGF;

[0050] (b) adding the cell suspension dropwise onto the membrane matrix for culturing to form a cell layer on the membrane matrix, thereby obtaining a membrane matrix with a cell layer attached thereto, and forming a three-dimensional cell layer, namely, the 3D cell layer.

[0051] In one embodiment, in step (b), the cell concentration in the cell suspension is (1-10)×10 5 / ml; the amount of cell suspension added is (1~5)×10 4 pieces / cm 2 ; If the medium material is used for brain-computer interface, the culture time is 14 to 21 days; if the medium material is used for brain-spinal interface, the culture time is 5 to 7 days.

[0052] In one embodiment, if the medium material is used for an eye-machine interface, the cells are allogeneic umbilical cord mesenchymal stem cells or allogeneic amniotic membrane mesenchymal stem cells;

[0053] The 3D cell layer is prepared by the following method:

[0054] (1) culturing and passaged allogeneic umbilical cord mesenchymal stem cells or allogeneic amniotic membrane mesenchymal stem cells to obtain P2 to P4 cells, and then resuspending them in DMEM / F12 low-glucose medium to obtain an allogeneic umbilical cord mesenchymal stem cell suspension or an allogeneic amniotic membrane mesenchymal stem cell suspension;

[0055] (2) adding an allogeneic umbilical cord mesenchymal stem cell suspension or an allogeneic amniotic membrane mesenchymal stem cell suspension onto the membrane matrix for culture to form a cell layer on the membrane matrix, thereby obtaining a membrane matrix with a cell layer attached, forming a three-dimensional cell layer, namely, the 3D cell layer.

[0056] In one embodiment, in step (2), the cell concentrations of the allogeneic umbilical cord mesenchymal stem cell suspension or the allogeneic amniotic membrane mesenchymal stem cell suspension are (1-10)×10 5 / ml; the amount of dripping is (1~5)×10 4 pieces / cm 2 ; The culture time is 24 to 30 days;

[0057] If the eye-machine interface is located at the cornea, the DMEM / F12 low-glucose culture medium contains the following components: 10% fetal bovine serum, 1% penicillin / streptomycin, 1020 ng / mL EGF, 510 ng / mL KGF, 15 ng / mL TGFβ, 5 μg / mL insulin, 0.4 μg / mL hydrocortisone, and 50 μg / mL ascorbic acid;

[0058] If the eye-machine interface is located at the retina, the DMEM / F12 low-glucose culture medium contains the following components: 2% B27 supplement, 1% non-essential amino acids, 1% L-glutamine, 1% penicillin / streptomycin, 1020ng / mL Activin A, 1020ng / mL BMP4, 50100ng / mL DKK1, 10ng / mL FGF2 and 0.51μM retinoic acid.

[0059] Another embodiment of the present invention provides a dielectric product for a brain-computer interface, a brain-spinal interface, or an eye-computer interface. The dielectric product comprises a transparent film, the dielectric material, a basement membrane, and a foil film laminated sequentially. The transparent film, basement membrane, and foil film are configured so that the dielectric material is protected and the dielectric material is properly oriented, rather than being used as a transplant material.

[0060] Another embodiment of the present invention provides the use of the aforementioned dielectric material or dielectric product in the preparation of a brain-computer interface, a brain-spinal interface, or an eye-computer interface. These interfaces primarily rely on signal transmission from retinal cells, interacting with the brain by capturing retinal electrical signals or directly stimulating them. The cornea's role in the eye-computer interface is primarily to assist in focusing and collecting light signals, rather than directly participating in signal transmission.

[0061] By wrapping the chip with the above-mentioned dielectric material, the immune rejection reaction between the implanted chip and human tissue can be reduced, the service life can be extended, and the contact method between the implanted chip and brain tissue can be optimized to ensure the stability and integrity of signal acquisition, avoid signal abnormalities, and at the same time reduce the risk of infection and prevent the formation of glial scar tissue.

[0062] Yet another embodiment of the present invention provides a brain-computer interface, a brain-spinal interface, or an eye-computer interface, which includes the above-mentioned dielectric material and chip.

[0063] In one embodiment, the dielectric material wraps the chip.

[0064] In one embodiment, the chip is a flexible chip or a rigid chip.

[0065] The technical solution of the present invention is further described in detail below through specific embodiments.

[0066] The raw materials used in the following examples are as follows:

[0067] Membrane matrix: Membrane matrix Cell Seed (セルシ-ド) was purchased from CellSeed Co., Ltd.

[0068] Barrier film: Purchased from Terumo; products include anti-adhesion materials for surgical procedures. AdSpray, trade name, is a medical material used to prevent postoperative adhesions and has been approved for manufacture and sale in Japan. Market Situation: Terumo's anti-adhesion materials are highly recognized in the market and are widely used in surgical procedures, particularly abdominal and pelvic surgeries, to reduce the occurrence of postoperative adhesions.

[0069] Basement membrane: Part of the material of CureAid Elastic fabric plaster (trade name: CureAid).

[0070] Example 1

[0071] This embodiment is a dielectric material for a brain-spinal interface, the dielectric material comprising a 3D cell layer and an isolation membrane bonded to the 3D cell layer;

[0072] The 3D cell layer is prepared from a membrane matrix and cells; the cells are autologous oral mucosal epithelial cells;

[0073] The 3D cell layer is prepared by the following method:

[0074] (a) The cells were cultured and passaged to obtain P3 cells, which were then resuspended in DMEM / F12 medium to obtain a cell suspension. The cell concentration in the cell suspension was 5×10 5 / ml; DMEM / F12 medium contains the following components: 10% fetal bovine serum, 1% penicillin / streptomycin and 510ng / mL bFGF;

[0075] (b) According to the drop amount of 2×10 4 pieces / cm 2 The cell suspension is added dropwise to the membrane matrix and cultured for 6 days. During the culture process, the culture medium is replaced every 3 days to form a cell layer on the membrane matrix, thereby obtaining a membrane matrix with a cell layer attached to form a three-dimensional cell layer, namely the 3D cell layer.

[0076] Example 2

[0077] This embodiment is a dielectric material for a brain-computer interface, the dielectric material comprising a 3D cell layer and an isolation membrane bonded to the 3D cell layer;

[0078] The 3D cell layer is prepared from a membrane matrix and cells; the cells are allogeneic umbilical cord mesenchymal stem cells;

[0079] The 3D cell layer is prepared by the following method:

[0080] (a) The cells were cultured and passaged to obtain P4 cells, which were then resuspended in DMEM / F12 medium to obtain a cell suspension. The cell concentration in the cell suspension was 5×10 5 DMEM / F12 medium contains the following components: 10% fetal bovine serum, 1% penicillin / streptomycin and 510 ng / mL bFGF

[0081] (b) The drop amount is 5×10 4 pieces / cm 2 The cell suspension is added dropwise to the membrane matrix and cultured for 16 days. During the culture process, the culture medium is replaced every 4 days to form a cell layer on the membrane matrix, thereby obtaining a membrane matrix with a cell layer attached to form a three-dimensional cell layer, namely the 3D cell layer.

[0082] Example 3

[0083] This embodiment is a dielectric material for an eye-machine interface, the eye-machine interface being located at the cornea, and the dielectric material comprising a 3D cell layer;

[0084] The 3D cell layer is prepared from a membrane matrix and cells; the cells are allogeneic umbilical cord mesenchymal stem cells;

[0085] The 3D cell layer is prepared by the following method:

[0086] (a) The cells were cultured and passaged to obtain P4 cells, which were then resuspended in DMEM / F12 low-glucose medium to obtain a cell suspension, wherein the cell concentration in the cell suspension was 5×10 5 / ml; DMEM / F12 low-glucose medium containing the following components: 10% fetal bovine serum, 1% penicillin / streptomycin, 1020ng / mL EGF, 510ng / mL KGF, 15ng / mL TGFβ, 5μg / mL insulin, 0.4μg / mL hydrocortisone, and 50μg / mL ascorbic acid;

[0087] (b) The drop amount is 5×10 4 pieces / cm 2The cell suspension is added dropwise to the membrane matrix and cultured for 24 days. The culture medium is replaced every 4 days during the culture process to form a cell layer on the membrane matrix, thereby obtaining a membrane matrix with a cell layer attached to form a three-dimensional cell layer, namely the 3D cell layer.

[0088] Example 4

[0089] This embodiment is a dielectric material for an eye-machine interface, the eye-machine interface being located at the retina, the dielectric material comprising a 3D cell layer;

[0090] The 3D cell layer is prepared from a membrane matrix and cells; the cells are allogeneic umbilical cord mesenchymal stem cells;

[0091] The 3D cell layer is prepared by the following method:

[0092] (a) The cells were cultured and passaged to obtain P4 cells, which were then resuspended in DMEM / F12 low-glucose medium to obtain a cell suspension, wherein the cell concentration in the cell suspension was 5×10 5 / ml; DMEM / F12 low-glucose medium containing the following components: 2% B27 supplement, 1% non-essential amino acids, 1% L-glutamine, 1% penicillin / streptomycin, 1020ng / mL Activin A, 1020ng / mL BMP4, 50100ng / mL DKK1, 10ng / mL FGF2, and 0.51μM retinoic acid;

[0093] (b) The drop amount is 5×10 4 pieces / cm 2 The cell suspension is added dropwise to the membrane matrix and cultured for 24 days. The culture medium is replaced every 4 days during the culture process to form a cell layer on the membrane matrix, thereby obtaining a membrane matrix with a cell layer attached to form a three-dimensional cell layer, namely the 3D cell layer.

[0094] Example 5

[0095] This embodiment is a medium product for brain-computer interface or brain-spinal interface, such as Figure 1 As shown, the dielectric product includes a transparent film, the dielectric material of Example 1, a base film and a foil film which are laminated in sequence.

[0096] Example 6

[0097] This embodiment is a dielectric product for a brain-computer interface or a brain-spinal interface, and the dielectric product includes a transparent film, the dielectric material of Example 2, a base film, and a foil film, which are laminated in sequence.

[0098] Example 7

[0099] This embodiment is a dielectric product for an eye-machine interface, which includes a transparent film, the dielectric material of embodiment 3, a base film, and a foil film laminated in sequence.

[0100] Example 8

[0101] This embodiment is a brain-computer interface or brain-spinal interface, which includes the dielectric material and chip of embodiment 1;

[0102] The dielectric material wraps the chip; the chip is a flexible chip.

[0103] Example 9

[0104] This embodiment is a brain-computer interface or brain-spinal interface, such as Figure 2 As shown, the brain-computer interface includes the dielectric material and chip of Example 1;

[0105] The dielectric material wraps the chip, and the chip is a hard chip.

[0106] Example 10

[0107] This embodiment is an eye-computer interface, which includes the dielectric material and chip of embodiment 3;

[0108] The dielectric material wraps the chip, and the chip is a hard chip.

[0109] Experimental example

[0110] 1. The dielectric material for brain-computer interface of Example 1 and the brain-computer interfaces of Examples 8 and 9 were implanted into the skull defect of the animal experiment. The implantation conditions were as follows: Figure 3 As shown; Figure 3 In the figure, from left to right, there are the pictures of the skull defect without membrane, the skull defect covered with dielectric material, the result picture of the brain-computer interface covered with Example 8 (soft chip membrane), and the result picture of the brain-computer interface covered with Example 9 (hard chip membrane).

[0111] 2. Implant the dielectric material for brain-computer interface of Example 3 and the eye-computer interface of Example 10 into the cornea. Figure 4 As shown; Figure 4 In the figure, from left to right are the uncoated cornea, the cornea coated with the dielectric material of Example 3, and the cornea coated with the eye-machine interface (hard chip film) of Example 10.

[0112] 3. The 3D cell layer prepared in Example 1, the 3D cell layer prepared in Example 4 and the blank membrane matrix were stained with DAPI nuclei respectively. The staining results are shown in FIG. Figures 5-7 As shown;

[0113] Figure 5This is the DAPI staining result of the 3D cell layer prepared in Example 1. Figure 6 This is the DAPI staining result of the blank membrane matrix; Figure 7 This is the DAPI staining result of the 3D cell layer prepared in Example 4;

[0114] Depend on Figures 5 to 7 It can be seen that the 3D cell layer of the present application forms a thin cell layer on the surface of the membrane matrix. Figure 7 After 24 days of culture, the 3D cell layer is thicker.

[0115] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the above embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the above embodiments, or make equivalent replacements for some or all of the technical features therein. These modifications or replacements do not deviate the essence of the corresponding technical solutions from the scope of the technical solutions of the embodiments of the present invention, and they should all be included in the scope of the claims and description of the present invention.

Claims

1. A dielectric material for a brain-computer interface, a brain-spinal interface, or an eye-computer interface, characterized in that: The medium material includes a 3D cell layer; The 3D cell layer is prepared from a membrane matrix and cells; the cells are selected from autologous oral mucosal epithelial cells, allogeneic umbilical cord mesenchymal stem cells or allogeneic amniotic membrane mesenchymal stem cells.

2. The dielectric material according to claim 1, wherein When the dielectric material is used for a brain-computer interface or a brain-spinal interface, the dielectric material further includes an isolation membrane adhered to the 3D cell layer.

3. The dielectric material according to claim 1, wherein If the medium material is used for a brain-computer interface or a brain-spinal interface, the cells are autologous oral mucosal epithelial cells, allogeneic umbilical cord mesenchymal stem cells, or allogeneic amniotic membrane mesenchymal stem cells; The 3D cell layer is prepared by the following method: (a) culturing and passaged the cells to obtain P2 to P4 cells, and then resuspending the cells in DMEM / F12 medium to obtain a cell suspension, wherein the DMEM / F12 medium contains the following components: 10% fetal bovine serum, 1% penicillin / streptomycin, and 510 ng / mL bFGF; (b) adding the cell suspension dropwise onto the membrane matrix for culturing to form a cell layer on the membrane matrix, thereby obtaining a membrane matrix with a cell layer attached thereto, and forming a three-dimensional cell layer, namely, the 3D cell layer.

4. The dielectric material according to claim 3, characterized in that In the step (b), the cell concentration in the cell suspension is (1-10)×10 5 / ml; the amount of cell suspension added is (1~5)×10 4 pieces / cm 2 ; If the medium material is used for brain-computer interface, the culture time is 14 to 21 days; if the medium material is used for brain-spinal interface, the culture time is 5 to 7 days.

5. The dielectric material according to claim 1, wherein If the medium material is used for an eye-machine interface, the cells are allogeneic umbilical cord mesenchymal stem cells or allogeneic amniotic membrane mesenchymal stem cells; The 3D cell layer is prepared by the following method: (1) culturing and passaged allogeneic umbilical cord mesenchymal stem cells or allogeneic amniotic membrane mesenchymal stem cells to obtain P2 to P4 cells, and then resuspending them in DMEM / F12 low-glucose medium to obtain an allogeneic umbilical cord mesenchymal stem cell suspension or an allogeneic amniotic membrane mesenchymal stem cell suspension; (2) adding an allogeneic umbilical cord mesenchymal stem cell suspension or an allogeneic amniotic membrane mesenchymal stem cell suspension onto the membrane matrix for culture to form a cell layer on the membrane matrix, thereby obtaining a membrane matrix with a cell layer attached, forming a three-dimensional cell layer, namely, the 3D cell layer.

6. The dielectric material according to claim 5, characterized in that In the step (2), the cell concentrations in the allogeneic umbilical cord mesenchymal stem cell suspension or the allogeneic amniotic membrane mesenchymal stem cell suspension are (1-10)×10 5 / ml; the amount of dripping is (1~5)×10 4 pieces / cm 2 ; The culture time is 24 to 30 days; If the eye-machine interface is located at the cornea, the DMEM / F12 low-glucose culture medium contains the following components: 10% fetal bovine serum, 1% penicillin / streptomycin, 1020 ng / mL EGF, 510 ng / mL KGF, 15 ng / mL TGFβ, 5 μg / mL insulin, 0.4 μg / mL hydrocortisone, and 50 μg / mL ascorbic acid; If the eye-machine interface is located at the retina, the DMEM / F12 low-glucose culture medium contains the following components: 2% B27 supplement, 1% non-essential amino acids, 1% L-glutamine, 1% penicillin / streptomycin, 1020 ng / mL ActivinA, 1020 ng / mL BMP4, 50100 ng / mL DKK1, 10 ng / mL FGF2 and 0.51 μM retinoic acid.

7. A medium product for a brain-computer interface, a brain-spinal interface, or an eye-computer interface, characterized in that: The dielectric product comprises a transparent film, the dielectric material according to any one of claims 1 to 5, a base film and a foil film which are laminated in sequence.

8. Use of the dielectric material according to any one of claims 1 to 6 or the dielectric product according to claim 7 in the preparation of a brain-computer interface, a brain-spinal interface or an eye-computer interface.

9. A brain-computer interface, brain-spinal interface, or eye-computer interface, characterized in that: The invention comprises the dielectric material and chip described in any one of claims 1 to 5.

10. The brain-computer interface, brain-spinal interface or eye-computer interface according to claim 9, characterized in that: The dielectric material wraps the chip.

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