Wastewater treating microbial agent and preparation method thereof

A microbial agent and wastewater treatment technology, applied in the field of environmental microorganisms, to achieve the effects of strong tolerance and adaptability, good performance and wide application range

Active Publication Date: 2013-10-30
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition to solving the problems existing in the existing short-range nitrification and denitrification, the microbial agent can be added to variou

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0025] Example 1 Preparation method of microbial agent

[0026] 1. Bacterial activation: The bacterial activation medium formula of C. marsh FSDN-A is: beef extract: 8g / L, peptone: 12g / L, NaNO 2 :0.5g / L, KNO 3 : 0.5g / L; The activation medium formula of Arthrobacter FDN-1 and Flavobacterium waters FDN-2 are: beef extract: 5g / L, peptone: 10g / L, NaNO 2 :1g / L, add 2.0% agar; the activation medium formula of Paracoccus denitrificans DN-3: KNO 3 :1g / L, sodium succinate: 8g / L, KH 2 PO 4 :1g / L, FeCL 2 : 0.5g / L, add 2.0% agar; the activation medium formula of Methylobacterium SDN-3 is: ammonium sulfate: 0.5g / L, methanol: 0.75mL / L, KH 2 PO 4 :1g / L, FeCL 2 : 0.5g / L,; add 2% agar. Spread evenly on the plate and place it in a constant temperature incubator at 30°C for activation.

[0027] 2. Scrape the bacteria on the plate with an inoculation ring and inoculate them in the corresponding liquid culture solution, and culture for 1 to 3 days to the logarithmic growth phase under aerobic condition...

Example Embodiment

[0030] Example 2 Application in a wastewater treatment system

[0031] Influent NH of a wastewater treatment system 3 -N concentration averages 320mg / L, total nitrogen (TN) concentration averages 512mg / L, COD Cr The average concentration is 661 mg / L. During operation, the average removal rate of ammonia nitrogen was 70%, the average removal rate of total nitrogen was only 48%, and the average removal rate of COD was 69%. Take a certain amount of liquid bacterial suspension A and add it to the aeration tank at a time of 5% (volume). After 30 days of operation, the average removal rate of ammonia nitrogen is 92%, the average removal rate of total nitrogen is 87%, and COD The average removal rate is 94%, and the sludge concentration is reduced by 5% compared to before the addition of bacteria. The addition of the bacteria increases the removal rate of total nitrogen and COD in wastewater. At the same time, the sludge concentration of the wastewater treatment system is running. Th...

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PUM

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Abstract

The invention discloses a wastewater treating microbial agent and a preparation method thereof. The microbial agent contains kocuriapalustris FSDN-A, arthrobactercreatinolyticus FDN-1, flavobacteriummizutaii FDN-2, paracoccusdenitrificans DN-3 and methylobacteriumphyllosphaerae SDN-3, wherein the collection register numbers of the five strains are respectively CGMCCNo.5061, CGMCCNo.3657, CGMCCNo.3659, CGMCCNo.3658 and CGMCCNo.3660. The microbial agent can achieve removal of ammonia nitrogen, total nitrogen and CODcr in the same reactor, has a good wastewater treatment effect, and can achieve short-cut nitrification and denitrification or simultaneous nitrification and denitrification while removing COD. Kocuriapalustris FSDN-A can utilize the two nitric oxides including nitrite nitrogen and nitric nitrogen, can ensure thorough denitrification of a wastewater treatment system, is slow in appreciation in the process of competing for substrates, is favorable for reduction of sludge, and further widens the application range of the microbial agent.

Description

technical field [0001] The invention belongs to the field of environmental microorganisms, and in particular relates to a microbial bacterial agent for wastewater treatment and a preparation method thereof. The bacterial agent can complete the removal of ammonia nitrogen, total nitrogen and CODcr, and realize the industrialization and production of microbial bacterial agents for wastewater treatment. commoditization. Background technique [0002] Biological denitrification is one of the more cost-effective methods to solve nitrogen pollution. Whether it is a traditional microbial-attached sewage treatment structure or some newly developed high-efficiency biofilm treatment systems, the microorganisms responsible for denitrification are mainly autotrophic nitrifying bacteria. The proliferation rate of autotrophic bacteria is slow, they cannot compete with heterotrophic bacteria in the mixed culture activated sludge system, it is difficult to obtain high biomass, and the nitri...

Claims

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Application Information

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IPC IPC(8): C12N1/00C12N1/20C02F3/34C12R1/01C12R1/06C12R1/20
Inventor 高会杰孙丹凤许谦
Owner CHINA PETROLEUM & CHEM CORP
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