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Tissue culture and rapid propagation method for corydalis saxicola bunting

A tissue culture and Coptis chinensis technology, applied in the field of plant reproduction, can solve the problems of low seed germination rate and difficulty in realizing large-scale cultivation, and achieve the effects of high-efficiency tissue culture, improving the quality of seedlings, and improving the reproduction coefficient.

Active Publication Date: 2014-02-05
GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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Problems solved by technology

[0003] In China, Yanhuanglian mainly relies on seeds for breeding, but the germination rate of seeds is low, and it is difficult to achieve large-scale cultivation in production.

Method used

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  • Tissue culture and rapid propagation method for corydalis saxicola bunting

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Embodiment Construction

[0017] The technical solutions of the present invention will be further described below in conjunction with the examples.

[0018] The rapid propagation method of Litectinus chinensis tissue culture of the present invention comprises the following steps:

[0019] (1) Selection and disinfection of explants: take the lateral buds of Litectinus chinensis as explants, soak them in 2% aqueous solution of detergent for 5-15 minutes, rinse them with linear tap water for 10-20 minutes, add 2-3 drops of Tween- 100 milliliters of 0.1% mercury liter at 20 minutes was sterilized for 8-10 minutes, rinsed with sterile water for 3-5 times, and finally the surface moisture was sucked off with sterile filter paper to obtain explants, wherein the sterile water was distilled water sterilized by high pressure;

[0020] (2) Differentiation of lateral buds to obtain clustered buds and rapid propagation: Inoculate the explants obtained in step (1) into MS medium at a culture temperature of 23-26°C, ...

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Abstract

The invention provides a tissue culture and rapid propagation method for corydalis saxicola bunting. The tissue culture and rapid propagation method comprises the following steps: (1) taking a corydalis saxicola bunting lateral bud as an explant and disinfecting; (2) putting the disinfected explant into an MS (Murashige and Skoog) culture medium to be induced and burgeoned to obtain cluster buds; (3) putting the cluster buds into an MS strong culture medium to be subjected to strong seedling culture to obtain a strong plant; (4) putting the strong plant into an MS rooting culture medium to be cultured to obtain a complete seedling with roots; and (5) taking the complete seedling with the roots and carrying out domestication; and transplanting the seedling into a sand bed to grow for one and a half months and then transplanting the seedling to a large field. With the adoption of the culture method, an obtained germchit is strong and the survival rate is high; a lot of high-quality germchits of the corydalis saxicola bunting can be provided in short time so that the large-scale seedling growth problem of the corydalis saxicola bunting is solved effectively.

Description

technical field [0001] The invention relates to a method for plant propagation, in particular to a method for rapid propagation of rock berthia by tissue culture. Background technique [0002] Corydalis saxicola Bunting, also known as Corydalis saxicola Bunting, is a perennial herb of the genus Corydalis in the family Corydalis. The whole herb contains active ingredients such as dehydrocarvitin (petidine); it has significant antibacterial, anti-inflammatory, analgesic and strong stabilizing effects, and it has been observed to inhibit tumor cells; it is mainly used to treat sores, furuncles, hepatitis, and liver cirrhosis , liver cancer and other diseases. The plant distribution of Yanhuanglian is limited to limestone mountainous areas, and it is an endemic species of Shishan. Due to the poor habitat conditions, its natural reproduction rate is very low, the population development is difficult, and the resource reserves are very limited. With the continuous improvement of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 李林轩韦坤华缪剑华李翠韦莹
Owner GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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