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Method for investigating estrogen active constribution material in sewage

An estrogen and contribution technology, applied in the field of detection of trace estrogen activity contribution in sewage, can solve the problems of cumbersome steps and difficult analysis, and achieve the effect of simple process and elimination of interference effect.

Inactive Publication Date: 2007-07-18
TSINGHUA UNIV
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Problems solved by technology

[0004] The purpose of the present invention is to aim at the shortcomings of using instrument test-oriented grading technology to analyze the main estrogen activity contributors in sewage, the steps are cumbersome, and the analysis is difficult. Estrogenic active substances in the medium, to achieve effective determination of the main estrogenic active contributors in sewage

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  • Method for investigating estrogen active constribution material in sewage

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Embodiment Construction

[0016] The technical solution of the present invention will be further described below in conjunction with the embodiments and the drawings.

[0017] Collect a good instantaneous sewage sample or a 24h mixed sample, and take 1L through a 0.7μm glass fiber filter membrane to remove the interference of suspended particles in the water. The filtered water sample is extracted with a solid phase extraction cartridge to extract the active substances of estrogen. In order to improve the extraction efficiency of the extraction cartridge and avoid the interference of water, the extraction cartridge needs to be freeze-dried, using n-hexane, dichloromethane and Elute the estrogenic active substances in the extraction cartridge with methanol, combine the eluates, and pass the two-stage silica gel-alumina chromatography column, including 10g silica gel and 10g alumina, and wash the silica gel with n-hexane, dichloromethane and methanol. -Alumina chromatographic column, eluted to obtain three c...

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Abstract

The invention relates to a method used to detect estrogen activity contribution matter in sewage. It includes the following steps: extracting estrogen activity matter form the sewage sample by solid phase column extractor; using methanol, dichloroethanes, normal hexane purging, and silica gel chromatographic column to classify into polarity, moderate and weak polarity three components; using recombination gene yeast bioassay method to measure the estrogen activity; taking out the maximum component to analyze its kind and density by gas phase color photography-mass spectrum on line. This method can fast effectively detect the main estrogen activity contribution matter in the sewage, supply foundation and support for sewerage treatment technology improvement.

Description

Technical field [0001] The invention relates to the technical field of detection of organic pollutants in sewage, in particular to a detection method of trace estrogen active contributors in sewage. Background technique [0002] At present, endocrine disruptors in water have attracted widespread attention at home and abroad, and endocrine disruptors with estrogen activity occupy a major position. There are hundreds of these substances, and the known ones mainly include pesticides (atrazine, DDT, toxaphene, etc.), natural estrogen (17-beta estradiol, estrone, etc.), surfactants (alkyl Phenol polyoxyethylene ethers and their metabolites, such as nonylphenol, octylphenol, etc.), drugs (diethylstilbestrol, ethinyl estradiol, etc.) and industrial products (phthalates, bisphenol A, etc.). With the rapid development of industry and agriculture and the acceleration of urbanization, these substances are mixed in a large amount of industrial sewage and domestic sewage. Without effective se...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N33/00G01N30/08C12Q1/00
Inventor 余刚孙庆峰邓述波
Owner TSINGHUA UNIV
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