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Rapid breeding method of lamina seedlings of tinospora capillipes gagnep

A technology of Lavender japonica and leaves, which is applied in the field of plant propagation, can solve the problems of low natural reproduction rate, scarcity of seeds and seedlings, and low natural seed setting rate, and achieve the goal of increasing the multiplication coefficient, improving the quality of seedlings, and realizing large-scale production Effect

Inactive Publication Date: 2015-04-01
GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the same time, the golden fruit olive is a dioecious plant, the natural seed setting rate is low, the natural reproduction rate is low, the seeds and seedlings are scarce, and resource renewal is very difficult
Although some scientific research workers have conducted some research on the cutting propagation technology of jinguolan, but the effect is not very obvious, and it cannot meet the needs of artificial cultivation.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] An example of the rapid breeding method of Lavender oleracea leaf becoming seedling of the present invention, comprises the following steps:

[0016] (1) Leaf differentiation and reproduction to obtain clustered buds: take the young leaves of the aseptic test-tube seedlings of Lavender, use a scalpel to scratch the back of the leaves in a # shape, and spread them on the MS propagation medium, at a culture temperature of 23-27 ℃, light intensity 1000lux, and light time 6-8 hours / day under the condition of cultivating for 30 days to obtain test-tube plantlets clustered buds, wherein the MS propagation medium is MS basic medium with 0.5 mg / L 6-benzyl adenine added 6-BA, 0.5mg / L indolebutyric acid IBA, 0.1mg / LTDZ thioridazine, 1.0mg / LGA3 gibberellin, 30g / L sucrose and 3.4g / L agar, the pH value of the medium is 4.0, the proliferation coefficient of clustered buds is 26.4;

[0017] (2) Cultivation of clustered buds and strong seedlings: the test tube plantlets clustered buds...

Embodiment 2

[0020] Another example of the rapid breeding method of Lavender oleracea leaf becoming seedling of the present invention, comprises the following steps:

[0021] (1) Leaf differentiation and reproduction to obtain clustered buds: take the young leaves of the aseptic test-tube seedlings of Lavender, use a scalpel to scratch the back of the leaves in a # shape, and spread them on the MS propagation medium, at a culture temperature of 23-27 ℃, light intensity 1000lux, and light time 6-8 hours / day under the conditions of culturing for 30 days to obtain test tube plantlets with clustered buds, wherein the MS propagation medium is MS basic medium with 1.0 mg / L 6-benzyl adenine added 6-BA, 0.25mg / L indolebutyric acid IBA, 0.5mg / LTDZ thioridazine, 1.0mg / LGA3 gibberellin, 30g / L sucrose and 3.4g / L agar, the pH of the culture medium is 4.2, the proliferation coefficient of clustered buds is 28.6;

[0022] (2) Cultivation of clustered buds and strong seedlings: the test tube plantlets cl...

Embodiment 3

[0025] Another example of the rapid breeding method of Lavender oleracea leaf becoming seedling of the present invention, comprises the following steps:

[0026] (1) Leaf differentiation and reproduction to obtain clustered buds: take the young leaves of the aseptic test-tube seedlings of Lavender, use a scalpel to scratch the back of the leaves in a # shape, and spread them on the MS propagation medium, at a culture temperature of 23-27 ℃, light intensity 1000lux, and light time 6-8 hours / day under the conditions of culturing for 30 days to obtain test-tube plantlets with clustered buds, wherein the MS propagation medium is MS basic medium with 1.5 mg / L of 6-benzyl adenine added 6-BA, 0.5mg / L indolebutyric acid IBA, 0.25mg / LTDZ thioridazine, 1.0mg / LGA3 gibberellin, 30g / L sucrose and 3.4g / L agar, the pH of the culture medium is 4.3, the proliferation coefficient of clustered buds is 32.8;

[0027] (2) Cultivation of clustered buds and strong seedlings: the test tube plantlets...

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Abstract

The invention discloses a rapid breeding method of lamina seedlings of tinospora capillipes gagnep. The method comprises the steps: (1) scratching the back sides of tender laminas of sterile test tube seedlings of tinospora capillipes gagnep to be well-shaped by using a scalpel and putting into an MS propagation medium for culturing; performing induction formation on callus and classifying to obtain multiple shoots; (2) putting the multiple shoots into an MS strong seedling medium for culturing strong seedlings to obtain strong plants; and (3) putting the strong plants into a 1 / 2 MS rooting medium to obtain complete root seedlings. The proliferation coefficient of the multiple shoots of the tinospora capillipes gagnep obtained by using the culturing method reaches 20-30 times, the rooting rate of the obtained tissue culture seedlings is more than 95 percent, the survival rate of seedbed transplanting is more than 90 percent, and the scale seedling breeding problem of the tinospora capillipes gagnep is effectively solved.

Description

technical field [0001] The invention relates to a method for plant propagation, in particular to a rapid propagation method for forming seedlings from Lavender oleifera leaves. Background technique [0002] Tinospora capillipes Gagnep. is a twining vine of the genus Tinospora genus Tinospora capillipes Gagnep. The medicinal part is its dry tuberous root. It is one of the base plants of Tinospora capillipes Gagnep. It has the effects of clearing heat and detoxification, relieving sore throat and relieving pain. It is mainly used for oral administration to treat various inflammations, such as sore throat, tonsillitis, acute gastroenteritis, bacillary dysentery, carbuncle, furuncle, etc.; Grind it into powder and apply it on the skin to treat phlebitis, poisonous snake bites and other diseases. It is mainly produced in Guangxi, Jiangxi, Hubei, Hunan and other places. It is a commonly used traditional Chinese medicine. The root contains tetrandrine palmatum and currumbin, palma...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 王晓峰韦坤华缪剑华李林轩李翠韦莹王一诺冯世鑫
Owner GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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