Tissue culture rapid-multiplying method for bletilla striata protocorm-like bodies

A protocorm-like and tissue culture technology, applied in the field of plant reproduction, can solve the problems of long time required, limited number of seedlings, low survival rate, etc., and achieve the effects of realizing large-scale production, reducing cost consumption and improving multiplication coefficient.

Active Publication Date: 2014-02-05
GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the scarcity of seedlings is the key problem restricting the popularization of white and artificial planting.
The seeds of Baiji are small, and when the seeds mature, they are mostly in the proembryo stage. Under natural conditions, they need to coexist with suitable fungi, the germination rate is extremely low, and the cultivation of seedlings is also relatively difficult.
In addition, although traditional breeding can also be propagated through asexual reproduction methods such as cuttings and divisions, the number of seedlings obtained is very limited, the survival rate is extremely low, and the time required is very long. It is difficult to achieve large-scale cultivation in production.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] A rapid propagation method for tissue culture of white and protocorm-like, comprising the following steps:

[0017] (1) Rapid propagation and cultivation of protocorms: take white and sterile test-tube seedlings, cut them into individual protocorms with seedlings, inoculate them into MS propagation medium, and cultivate them at a culture temperature of 23-27°C and a light intensity of 1500 lux. Protocorm-like stems were obtained by culturing for 30 days under the condition of 12-14 hours / day of light, wherein the MS propagation medium contained 1.5 mg / L of 6-benzyl adenine 6-BA and 0.1 mg / L of indole acetic acid The agar of the kinetin KT of IAA, 0.1mg / L, 30g / L sucrose and 3.4g / L, the pH value of culture medium is 5.8, and the proliferation coefficient of pseudocorm is 20.5;

[0018] (2) Rejuvenation culture of protocorm-like: place the protocorm-like obtained in step (1) in MS strong seedling medium at a culture temperature of 23-27°C, light intensity of 1500lux, and l...

Embodiment 2

[0022] A rapid propagation method for tissue culture of white and protocorm-like, comprising the following steps:

[0023] (1) Rapid propagation and cultivation of protocorms: take white and sterile test-tube seedlings, cut them into individual protocorms with seedlings, inoculate them into MS propagation medium, and cultivate them at a culture temperature of 23-27°C and a light intensity of 1500 lux. Protocorm-like stems were obtained by culturing for 30 days under the condition of 12-14 hours / day of light, wherein the MS propagation medium contained 3.25 mg / L of 6-benzyladenine 6-BA and 0.3 mg / L of indole acetic acid The agar of the kinetin KT of IAA, 0.3mg / L, 30g / L sucrose and 3.4g / L, the pH value of culture medium is 5.8, and the proliferation coefficient of pseudocorm is 26.8;

[0024] (2) Rejuvenation culture of protocorm-like: place the protocorm-like obtained in step (1) in MS strong seedling medium at a culture temperature of 23-27°C, light intensity of 1500lux, and l...

Embodiment 3

[0028] A rapid propagation method for tissue culture of white and protocorm-like, comprising the following steps:

[0029] (1) Rapid propagation and cultivation of protocorms: take white and sterile test-tube seedlings, cut them into individual protocorms with seedlings, inoculate them into MS propagation medium, and cultivate them at a culture temperature of 23-27°C and a light intensity of 1500 lux. Protocorms were obtained by culturing for 30 days under the condition of 12-14 hours / day of light, wherein the MS propagation medium contained 5.0 mg / L of 6-benzyladenine 6-BA and 0.5 mg / L of indole acetic acid The agar of the kinetin KT of IAA, 0.5mg / L, 30g / L sucrose and 3.4g / L, the pH value of culture medium is 5.8, and the proliferation coefficient of pseudocorm is 35.7;

[0030](2) Rejuvenation culture of protocorm-like: place the protocorm-like obtained in step (1) in MS strong seedling medium at a culture temperature of 23-27°C, light intensity of 1500lux, and light time of...

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PUM

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Abstract

The invention relates to a tissue culture rapid-multiplying method for bletilla striata protocorm-like bodies. The method comprises the following steps: (1) slitting bletilla striata and sterile test-tube plantlets into single corms with plantlets, inoculating to an MS protocorm-like body multiplying culture medium for multiplying culture to obtain protocorm-like bodies; (2) putting the obtained protocorm-like bodies into an MS rejuvenation culture medium for rejuvenation culture to obtain protocorms with seedlings; and (3) inoculating the protocorms with seedlings onto a 1 / 2 MS rooting culture medium for rooting culture to obtain small robust plants. The bletilla striata protocorm-like bodies obtained by adopting the tissue culture rapid-multiplying method are high in rejuvenation coefficient, short in rejuvenation time, robust for the rejuvenated multiple buds, the high-quality bletilla striata seedlings with high survival rate can be provided within short period, and the large-scale seedling culture problem of the bletilla striata can be effectively solved.

Description

technical field [0001] The invention relates to a method for plant propagation, in particular to a rapid propagation method for tissue culture of white and protocorm-like plants. Background technique [0002] Baiji, also known as Lianjicao, Ruolan, Gangen, Baigen, Baili, Baiji, Zihui, is a plant of the genus Baiji in the family Orchidaceae. The dry tuber of Baiji is a traditional Chinese medicine in my country, and its medicinal history has a long history. Baiji is warm in nature, bitter, sweet, and pungent. It has the functions of nourishing the lungs, dispelling wind and dehumidification, clearing the orifices, relieving pain, reducing swelling and discharging pus, promoting muscle growth, and astringing sores. It is mainly used to treat colds, headaches, eyebrow bone pain, nasal congestion, Nasal sinusitis, toothache, leucorrhea, lung injury, hemoptysis, epistaxis, hemorrhage from gold sores, carbuncle, ulcer pain, burns from hot soup, wrinkled hands and feet, etc. Baij...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 韦坤华李林轩缪剑华李翠韦莹凌征柱王晓峰
Owner GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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