Quick tissue culture breeding and seedling raising method for narcissus tazetta var. chinensis

A fast, tissue culture technology, applied in the field of plant tissue culture, can solve the problems of the complete process of factory seedling raising, less attention and lack of production cost control in the production cycle, to shorten the production cycle, simplify the production steps, and shorten the production cycle. Effect

Active Publication Date: 2011-09-14
SHANGHAI BOTANICAL GARDEN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, domestic and foreign research on the high-quality seedling rapid propagation technology system of Chongming Narcissus is relatively lacking, and the focus of the research is mostly on the differentiation ability of different explants and the influence of different hormones on bulb induction, which will affect the complete process and production of industrial seedlings. Less attention is paid to the control of cycle time and production cost

Method used

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  • Quick tissue culture breeding and seedling raising method for narcissus tazetta var. chinensis
  • Quick tissue culture breeding and seedling raising method for narcissus tazetta var. chinensis

Examples

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Embodiment 1

[0031] 1) Selection and disinfection of explants. Select Chongming narcissus bulbs that are more than 2 years old with robust growth, no pests and diseases, and no virus symptoms, and store them at a low temperature of 4°C for 6 weeks. Remove the shrunken bulb epidermis and roots, add detergent to scrub 2 to 3 times, and rinse under running water for 30 minutes. Disinfect after cutting the bulbs horizontally along 1 / 2-1 / 3, first disinfect with 75% (v / v) alcohol for 90 seconds, then disinfect with 0.1wt% mercury liter for 10 minutes, and then wash with sterile water at 60°C for 3 Once again, disinfect with 0.1% mercuric chloride for 15 minutes, then wash with sterile water for 3 to 5 times, and set aside.

[0032] 2) Induction culture: under aseptic conditions, cut the sterilized bulb into 0.5-1.0 cm×1.0 cm size, keep 2-3 layers of bulb slices for each piece, and inoculate it on the induction medium. The culture conditions are: the temperature is 24±1° C., the light intensity...

Embodiment 2

[0036] Embodiment 2: industrialized seedling raising operation method

[0037] 1) Selection and disinfection of explants. Select Chongming narcissus bulbs that are more than 2 years old and have robust growth, no pests and diseases, and no virus symptoms, and store them at a low temperature of 4°C for 6 weeks. Remove the shrunken bulb epidermis and roots, add detergent to scrub 2 to 3 times, and rinse under running water for 30 minutes. Disinfect after cutting the bulbs horizontally along 1 / 2-1 / 3, first disinfect with 75% (v / v) alcohol for 90 seconds, then disinfect with 0.1wt% mercury liter for 10 minutes, and then wash with sterile water at 60°C for 3 Once again, disinfect with 0.1% mercuric chloride for 15 minutes, then wash with sterile water for 3 to 5 times, and set aside.

[0038] 2) Induction culture: cut the sterilized bulb into 0.5-1.0 cm×1.0 cm size, keep 2-3 layers of bulb slices for each piece, and inoculate it on the induction medium. The culture conditions ar...

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Abstract

The invention discloses a quick tissue culture breeding and seedling raising method for narcissus tazetta var. chinensis, which comprises the following steps of the selection and disinfection of explants, induction culture, multiplication culture, root induction culture and transplantation rooting. In the tissue culture propagation method for the narcissus tazetta var. chinensis, the complete technical process from the selection of the explants to the transplantation of the explants is accomplished, and bulbs subjected to the multiplication culture are rooted and induced within short time and are transplanted to a perlite substrate for rooting directly, so that test tube seedlings of the narcissus tazetta var. chinensis are not needed to be rooted in vitro; and the steps of induction rooting and transplantation are simplified, the production period is shortened, the cost is reduced, and the survival rate of the transplantation reaches over 92 percent. The method is high in pertinence, simple and easy and high in multiplication rate, produced seed bulbs are high in consistency and few in plant diseases and insect pests, the cost is saved, and the requirements for the industrial production of the narcissus tazetta var. chinensis seedlings can be met.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a Chongming narcissus tissue culture rapid propagation seedling raising method. Background technique [0002] Chongming narcissus (Narcissus tazetta var. chinensis) is a unique narcissus variety in Shanghai. It was once as famous as Zhangzhou narcissus, and it is one of the two major narcissus strains in my country. Chongming has a history of cultivating narcissus for more than 400 years and producing it as a commercial flower for more than 100 years. The characteristic of Chongming narcissus is that there are few natural branches of bulbs, the petals are as white as jade, the shape is like a disc, and the heart is golden, like a wine cup, so it has the good name of "Jalendula Silver Terrace". The fragrance of the flowers is strong, and it lasts for a month, which is not as good as other narcissus. Chongming narcissus is the main cultivated flower variety in Chongm...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G31/00
Inventor 刘炤胡永红陈必胜秦俊蔡新凤朱华芳张亚利殷丽青赵晓峰李建高燕
Owner SHANGHAI BOTANICAL GARDEN
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