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Lecanicilliumpsalliotae strain used for preventing and treating diaphorina citri

A technology of Verticillium ciferium and citrus psyllids, applied in the field of microorganisms, can solve the problems of root-knot nematode infection and achieve the effects of drug resistance, high spore germination rate, and easy preparation

Active Publication Date: 2013-08-14
ZHEJIANG CITRUS RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few domestic studies on this fungus, only reports about its infecting root-knot nematode
However, there has not been any report on the use of Verticillium ciferium to control citrus psyllids at home and abroad.

Method used

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  • Lecanicilliumpsalliotae strain used for preventing and treating diaphorina citri
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  • Lecanicilliumpsalliotae strain used for preventing and treating diaphorina citri

Examples

Experimental program
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Effect test

Embodiment 1

[0021] Example 1: Isolation and Screening of Entomopathogenic Bacteria

[0022]1. Isolation and purification of pathogens: Collect citrus psyllid carcasses from the citrus orchard in Huangyan District, Taizhou City, Zhejiang Province, and perform the following operations in the ultra-clean workbench of the laboratory: first soak the carcasses in 70% ethanol for 30 s, and then After soaking in 0.1% mercuric chloride for 3 minutes, rinse with sterile water for 3 times. Use sterile filter paper to dry the water on the worm body, and then put it on the potato dextrose agar (PDA) medium (preparation method: weigh 200 g potatoes, wash, peel and chop them, add 1000 mL of water and boil for half an hour , filter with gauze, add 20 g of glucose and 20 g of agar, fully dissolve, then filter with gauze while hot, and dispense into Erlenmeyer flasks or glass test tubes, sterilize under high pressure at 121°C for 20 min). Place the petri dish in a microbial incubator and incubate at a con...

Embodiment 2

[0026] Embodiment 2: Identification of bacterial strain ZJLP09

[0027] 1. Morphological characteristics of the strain ZJLP09: Inoculate the isolated and purified strain ZJLP09 into the center of the PDA medium plate, place it in a constant temperature culture at 25°C, observe the growth of the colony every day and record the color and shape of the colony. Inoculate the strain ZJLP09 into the center of another PDA medium plate, insert the sterilized cover glass (1 cm×1 cm) obliquely into the medium about 1 cm away from the inoculation point, and place it in a constant temperature culture at 25 °C About 5 days after the hyphae climbed onto the cover glass, the cover glass was taken out and placed under an optical microscope to observe the hyphae and spore morphology of the strain ZJLP09.

[0028] figure 1 The morphological characteristics of strain ZJLP09 were shown after being cultured on PDA medium at 25 ℃ for 7 days. It can be seen that the colony of strain ZJLP09 was roun...

Embodiment 3

[0032] Embodiment 3: the biological characteristic of bacterial strain ZJLP09

[0033] 1. The effect of different temperatures on the growth of strain ZJLP09: use a sterile puncher to punch 5 mm-sized bacterial cakes around the colonies of strain ZJLP09 cultured on PDA medium for about 7 days, and then place the bacterial cakes on several The center of each freshly prepared PDA plate (9 cm in diameter) was cultured upside down in microbial incubators at 5°C, 10°C, 15°C, 20°C, 25°C, 30°C, 35°C and 40°C, and each temperature Set up 3 repetitions. After culturing for 14 days, the growth of the colony was observed every 24 h and the diameter of the colony was recorded by the cross method.

[0034] The results are shown in Table 1. It can be seen that the strain ZJLP09 can grow on the PDA medium at a temperature of 10°C to 35°C, and the growth rate of mycelia at 25°C and 30°C is significantly higher than that at other temperatures, and the growth rate at 5°C and 30°C is significan...

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Abstract

The invention relates to a Lecanicilliumpsalliotae strain used for preventing and treating diaphorina citri and belongs to the technical field of microorganisms. The Lecanicilliumpsalliotae strain ZJLP09 is registered and preserved in China General Microbiological Culture Collection Center with the preservation number of CGMCCNo.7551. The Lecanicilliumpsalliotae strain ZJLP09 has strong pathogenicity on diaphorina citri; compared with a congeneric verticillum lecanii strain, the Lecanicilliumpsalliotae strain ZJLP09 has a stronger effect on the diaphorina citri and can be developed into a biological fungi-proofing preparation used for preventing and treating the diaphorina citri; and compared with the existing chemical agent used for preventing and treating the diaphorina citri, the Lecanicilliumpsalliotae strain ZJLP09 has the characteristics of high efficiency, low toxicity, low residue, no pollution and difficulty in formation of drug resistance and has a good market application prospect.

Description

technical field [0001] The present invention relates to a strain of Verticillium ciferium ( Lecanicillium psalliotae ) strain, which belongs to the technical field of microorganisms. Background technique [0002] Citrus Huanglongbing (HLB) is currently the most difficult and threatening destructive disease in citrus production. It is widely distributed in many countries and regions such as Asia, Africa and America. The rampant occurrence and spread of the disease has brought huge economic losses to people and has become a major obstacle to the stable development of the citrus industry. Although it has been more than 100 years since the disease was discovered, there is still a lack of effective control agents and ideal disease-resistant varieties. Popularity. The causative agent of the disease is Phloembacterium ( Candidatus Liberibacter) bacteria, mainly through citrus psyllids ( Diaphorina citri ) spread out. Citrus psyllid is not only the only natural insect vecto...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01P7/04C12R1/645
Inventor 鹿连明杜丹超胡秀荣蒲占湑陈国庆张小亚
Owner ZHEJIANG CITRUS RES INST
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