Phellinus igniarius bag cultivation medium and method for cultivating phellinus igniarius sporophore by same

The technology of culture medium and Phellinus linteus bag is applied in the biological field, which can solve the problems of small fruiting body development, easy contamination of miscellaneous bacteria, insufficient nutrient supply of culture medium, etc., and achieves the effects of stable quality, reduced waste and environmental protection.

Active Publication Date: 2012-11-21
ZHEJIANG QIANCAOSU BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In order to solve the shortage of wild Phellinus chinensis resources at present, there are problems such as insufficient nutrient supply of the culture medium in bag material cultivation in artificial cultivation, small fruit body development, and easy contamination of miscellaneous bacteria. The formula of Phellinus japonica bag material medium can provide the nutrients taken by the growth of Phellinus chinensis fruiting bodies;

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Configure 1 kilogram of phellinus fruiting body culture medium described in the present invention, each component weight of nutrient material in every kilogram is: mulberry branch sawdust 720 grams, mulberry leaf powder 100 grams, corn grain 100 grams, sucrose 10 grams, quicklime 50 grams grams, KH 2 PO 4 10 g, MgSO 4 10 grams; stir evenly according to the formula, add 1.8 kg of water, pack into bags after mixing, and the bagged culture medium is sterilized by autoclaving. Sterilization conditions: pressure 0.15MPa, temperature 112°C, time 180min, to obtain Phellinus phellinus bag material cultivation medium .

[0031] Inoculate the Phellinus phellinus mother species in the above-mentioned Phellinus phellinus bag material cultivation medium in the aseptic room, the inoculum amount is 10%, the bacterial stick culture temperature after inoculation is 26 ℃, air humidity 75%, bag material humidity 50%, light The intensity is 20lux, the frequency of ventilation is 2 times...

Embodiment 2

[0034] Configure 1 kilogram of phellinus fruiting body culture medium described in the present invention, each component weight of nutrient material in every kilogram is: 700 grams of mulberry branch sawdust, 100 grams of mulberry leaf powder, 135 grams of wheat grains, 15 grams of glucose, 20 grams of unslaked lime grams, KH 2 PO 4 15 g, MgSO 4 15 grams; stir evenly according to the formula, add 2.5 kg of water, pack into bags after mixing, and sterilize the bagged culture medium by autoclaving. Sterilization conditions: pressure 0.05MPa, temperature 121°C, time 120min, to obtain Phellinus phellinus bag material cultivation medium .

[0035] Inoculate the Phellinus phellinus female species in the above-mentioned Phellinus phellinus bag material cultivation medium in a sterile room, the inoculum amount is 15%, the bacterial stick culture temperature after inoculation is 28 ℃, the air humidity is 65%, the bag material humidity is 55%, and the light The intensity is 10lux, th...

Embodiment 3

[0038] Configure 1 kilogram of phellinus fruiting body culture medium described in the present invention, each component weight of nutrient material in every kilogram is: 650 grams of mulberry branch sawdust, 100 grams of mulberry leaf powder, 200 grams of rice, 10 grams of sucrose, 15 grams of quicklime , KH 2 PO 4 15 g, MgSO 4 10 grams; stir evenly according to the formula, add 2.3 kg of water, pack into bags after mixing, and the bagged culture medium is sterilized by autoclaving. Sterilization conditions: pressure 0.15MPa, temperature 128°C, time 90min, to obtain Phellinus phellinus bag material cultivation medium .

[0039] Inoculate the Phellinus Phellinus mother species into the above-mentioned Phellinus Phellinus bag material cultivation medium in the aseptic room, the inoculation amount is 20%, the bacterial stick culture temperature after inoculation is 26 ℃, the air humidity is 70%, the bag material humidity is 60%, and the light The intensity is 15lux, the frequ...

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PUM

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Abstract

The invention relates to the technical field of biology; in order to solve the deficiency of wild phellinus igniarius resources at present, and the problems of insufficient medium nutrition supply, small sporophore growth, and easy undesired microbe infection of bag cultivation in artificial cultivation, the invention provides a phellinus igniarius bag cultivation medium and a method for cultivating phellinus igniarius sporophores by using the medium; the phellinus igniarius bag cultivation medium is prepared by mixing nutrients with water, packaging the mixture in bags, sterilizing the bags at a temperature of 110-135 DEG C, at a pressure of 0.05-0.25 MPa, for 30-180 min, wherein the mass ratio of the nutrients to water is 1:1.8-2.5. With the phellinus igniarius bag cultivation medium formula of the invention, nutrients absorbed during phellinus igniarius sporophore growth are provided, and the cultivated phellinus igniarius sporophores are similar to wild phellinus igniarius with stable quality; and the cultivation mode of the invention is simple, and can be used for large scale cultivation of phellinus igniarius sporophores with medicinal value.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to the formulation of a culture medium for Phellinus phellinus bag material and a method for large-scale cultivation of Phellinus phellidis fruiting bodies by using the medium. Background technique [0002] Phellinus, Basidiomycotina, Phyllomycetes, Polyporacea, Polyporaceae, Pinfoliobacteria fungi. scientific name [0003] Phellinus Linteus, Chinese name Acupunculus clefthoof, commonly known as Sangchen, Phellinus lenteus, etc.; it is a medicinal fungus that parasitizes broad-leaved trees such as mulberry trees, and is known as "forest gold". According to the "Properties of Medicine" record: sweet and pungent, non-toxic. "National Compilation of Chinese Herbal Medicine" records that Phellinus has the effects of "strengthening five viscera, softening firmness, detoxification, hemostasis, promoting blood circulation and stomach diarrhea", and is currently internationally recognized as ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G1/00A01G1/04
Inventor 陈平华寿旦万和炎马相锋计东风李有贵方俊
Owner ZHEJIANG QIANCAOSU BIOTECH CO LTD
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