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Salt-tolerant COD removal denitrifying microbial agent and preparation method thereof

A microbial agent, denitrifying paracoccus technology, applied in the field of environmental microorganisms, can solve the problems of limited application range and limited processing capacity, and achieve the effects of broadening the scope of application, improving the composition, and strengthening the effect of biochemical treatment

Active Publication Date: 2017-05-10
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method mainly relies on denitrification to remove nitrite to deal with COD, the treatment capacity is limited, and the scope of application is also limited to a certain extent.

Method used

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  • Salt-tolerant COD removal denitrifying microbial agent and preparation method thereof
  • Salt-tolerant COD removal denitrifying microbial agent and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The preparation method of embodiment 1 microbial bacterial agent

[0026] (1) Inoculate Paracoccus FSTB-2, Microbacterium Kitami FSTB-4 and Pseudomonas stutzeri FSTB-5 on FSTB solid medium for activation respectively; denitrifying Paracoccus DN-3 and A Bacteria SDN-3 were respectively inoculated on the corresponding solid medium for activation; spread evenly and placed in a constant temperature incubator at 35°C for activation.

[0027] (2) Use the inoculation loop to inoculate the colonies of Paracoccus FSTB-2, Kitamibacterium FSTB-4 and Pseudomonas stutzeri FSTB-5 on the plate into the corresponding FSTB liquid medium respectively, and use the inoculation loop to Take the colonies of Paracoccus denitrificans DN-3 and Methylobacterium SDN-3 on the plate and inoculate them in the corresponding liquid medium respectively, and culture them with shaking at 30°C and 150rpm for 48 hours to the logarithmic growth phase to obtain liquid bacteria agent seed liquid.

[0028] (...

Embodiment 2

[0032] Example 2 Microbial agent treatment of salt-containing COD and nitrogen-containing wastewater

[0033] The high-salt wastewater produced by a chemical plant has a salt content of 2.5wt%, a COD of 2000mg / L, and a total nitrogen of 80mg / L. Cannot meet the standard. The salt-tolerant microbial agent prepared in Example 1 was added to the system according to 1% of the volume of wastewater treatment every day for a total of 10 days. The treatment effect is shown in Table 2.

[0034] Table 2 Treatment effects of different salt-tolerant bacteria agents

[0035]

[0036] It can be seen from Table 2 that after being treated with the combined bacterial agent prepared by the present invention, the COD of the effluent is always lower than 60 mg / L, and the total nitrogen of the effluent is always lower than 23 mg / L. However, under the same conditions with single bacteria treatment, the effluent COD is higher than 99mg / L. Using the bacterial suspension described in CN2012101306...

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Abstract

The invention relates to a salt-tolerant COD removal denitrifying microbial agent. The microbial agent consists of at least one of paracoccus sp FSTB-2, microbacterium kitamiense FSTB-4 and pseudomonas stutzeri FSTB-5, and further consists of at least one of paracoccus denitrificans DN-3 and methylobacterium phyllosphaerae SDN-3, wherein the paracoccus sp FSTB-2, the microbacterium kitamiense FSTB-4 and the pseudomonas stutzeri FSTB-5 are preserved in China General Microbiological Culture Collection Center on June 1, 2015, with preservation numbers of CGMCC No.10938, CGMCC No.10939 and CGMCC No.10940; and the paracoccus denitrificans DN-3 and the methylobacterium phyllosphaerae SDN-3 are disclosed in CN102465104A and CN102465103, and the preservation numbers of the paracoccus denitrificans DN-3 and the methylobacterium phyllosphaerae SDN-3 are CGMCC No.3658 and CGMCC No.3660. The microbial agent can be used for directly processing total nitrogen and COD in high-salinity wastewater, and the microbial agent can be also added to various biochemical reaction constructions so as to improve microbiological composition, optimize the salt tolerance of a microbial system in wastewater treatment and improve total nitrogen and COD removal rate of the entire process.

Description

technical field [0001] The invention belongs to the field of environmental microorganisms, and in particular relates to a salt-tolerant COD and nitrogen-removing microbial bacterial agent and a preparation method thereof. Industrialization and commercialization of microbial agents for wastewater treatment. Background technique [0002] Highly saline wastewater with a salt content higher than 1% from petroleum, chemical, pharmaceutical, and fertilizer industries generally contains high COD, and the continuous discharge of pollutants in wastewater into the environment will cause serious pollution problems. The shortage of fresh water resources is becoming more and more serious, and the treatment and recycling of waste water have been paid more attention by people. [0003] Physical or chemical treatment of COD in high-saline wastewater is costly and may cause secondary pollution. Traditional biological methods have great advantages in treating low-salinity wastewater, but in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/38C12R1/01
Inventor 郭志华高会杰孙丹凤赵胜楠李宝忠
Owner CHINA PETROLEUM & CHEM CORP
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