Nitrite bacterium culture promoter and preparation method thereof

A technology of nitrous acid bacteria and accelerators, which is applied in the direction of bacteria, chemical instruments and methods, and the addition of compounds to stimulate growth, etc., can solve the problems of no promotion effect and slow growth of nitrous acid bacteria itself, and achieve enhanced reduction and shortened time. Enzyme-catalyzed reaction process, the effect of improving the treatment effect

Active Publication Date: 2016-06-01
CHINA PETROLEUM & CHEM CORP +1
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Problems solved by technology

CN201110315549.6 discloses a short-range nitrification and denitrification granular sludge cultivation method, which is characterized in that 5-15mg / L hydroxylamine is regularly added; CN201010168453.7 discloses a method for quickly starting an anoxic ammonia oxidation biofilter, It is characterized in that hydroxylamine is added in step (2) to induce the inoculated sludge to transform into anoxic ammonium oxidation biofilm. The above invention mainly utilizes the inhibitory effect of hydroxylamine on nitrite oxidizing bacteria to promote short-term nitrification, but slow growth The growth of nitrous bacteria does not promote the growth of

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  • Nitrite bacterium culture promoter and preparation method thereof
  • Nitrite bacterium culture promoter and preparation method thereof

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Embodiment Construction

[0019] The solutions of the present invention will be described in detail below in conjunction with specific embodiments.

[0020] The formulations of the nitrous bacteria growth promoters of Examples 1-6 and Comparative Examples 1-7 are shown in Table 1.

[0021] Formulation and ratio of accelerator of table 1

[0022]

[0023] Activated sludge from a sewage treatment plant was used as the inoculum to start the short-range nitrification process. During the start-up process, a certain concentration of solution was configured according to the formula in Table 1, and then added to Examples 1-6 and Comparative Examples 1-7 respectively. After adding, the accelerator concentration in the enriched culture solution was 15mg / L. After 30 days of start-up, the number of nitrous bacteria in the activated sludge was sampled with a microscope (counted by bacteria counting plate), and water samples were taken to analyze the nitrosation rate. The specific results See Table 2.

[0024] ...

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Abstract

The invention discloses a nitrite bacterium culture promoter, which consists of metal salts, polyamine substances, organic acid hydroxylamine and Na2SO3, wherein in parts by weight, the content of the metal salts is 40-100 parts by weight, preferably 50-80 parts by weight; the content of the polyamine substances is 5-30 parts by weight, preferably 10-20 parts by weight; the content of the organic acid hydroxylamine is 0.05-1.5 parts by weight, preferably 0.1-1.0 part by weight; and the content of the Na2SO3 is 10-40 parts by weight, preferably 20-30 parts by weight; the metal salts include calcium salt, ferrous salt and copper salt; and the molar ratio of Ca<2+> to Fe<2+> to Cu<2+> is (5-15) to (1-8) to (0.5-5), and preferably (8-12) to (2-6) to (1-4). The promoter is simple in formula and easy for preparing; and the promoter can be applied to the culture process of the nitrite bacteria and the promoter can be also directly added to a sewage treatment system, so that the starting of partial nitrification-denitrification and partial nitrification-anaerobic ammonia oxidation processes is accelerated and the stable running of the processes is achieved.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a nitrous bacteria cultivation accelerator and a preparation method thereof. Background technique [0002] Biological denitrification is mainly accomplished by nitrifying bacteria and denitrifying bacteria. Nitrifying bacteria are chemotrophic microorganisms. Biological cells can only use the energy stored in the form of ATP, etc., and cannot directly use the free energy released by chemical reactions. In aerobic metabolism, ATP is mainly synthesized by oxidative phosphorylation of the respiratory chain. The ammonia oxidative phosphorylation efficiency is very low, and the ATP that can be produced is very limited, and the energy is mainly used for electron transition to a higher energy level, which makes the growth of nitrifying bacteria very slow, and the generation period is 8~36h. The cell wall of nitrifying bacteria has low peptidoglycan content and high protein an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/38C12N1/20C02F3/34C02F3/30
Inventor 高会杰郭志华孙丹凤赵胜楠李宝忠
Owner CHINA PETROLEUM & CHEM CORP
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