Microorganism bacterium agent for treating leather wastewater and preparation method thereof

A technology for microbial inoculants and leather wastewater, applied in the directions of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of difficult removal of COD and ammonia nitrogen, and achieve improved sedimentation performance, simple operation and management, and strong impact resistance. Effects of Sexuality and Adaptability

Pending Publication Date: 2017-06-13
北京市科学技术研究院资源环境研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to solve the technical problem that COD and ammonia nitrogen existing in a large amount in leather wastewater are difficult to remove and provide a composite bacterial agent for treating leather wastewater and its preparation method

Method used

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  • Microorganism bacterium agent for treating leather wastewater and preparation method thereof
  • Microorganism bacterium agent for treating leather wastewater and preparation method thereof
  • Microorganism bacterium agent for treating leather wastewater and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] The effect of embodiment 1 microbial bacterial agent in synthetic waste water

[0036] Put the microbial agent in the medium containing molasses, incubate at 30°C, 110rpm for 12-24 hours, then centrifuge at 10,000rpm for 5min, discard the supernatant, wash twice with sterile water, centrifuge in the same steps, and remove the lower layer Bacteria were placed in synthetic wastewater at concentrations of 1%, 2%, and 4% (v / v) respectively. Synthetic wastewater components (g / L): glucose 1.0; ammonium sulfate 0.3; peptone 0.1; sodium bicarbonate 0.6; Potassium hydrogen 0.15; Magnesium sulfate heptahydrate 0.03; Manganese sulfate 0.01; Trace elements 1ml; Distilled water 1L. Three sets of parallel samples were made for each concentration. Put the synthetic waste water in the Erlenmeyer flask, inoculate the microbial agent into the synthetic waste water, place the Erlenmeyer flask in a shaker, shake the Erlenmeyer flask at a speed of 110rpm to make it oxygenated, and measure ...

Embodiment 2

[0038] Application effect of embodiment 2 microbial bacterial agents in leather wastewater

[0039] The embodiment provides a method for the enhanced treatment of actual leather wastewater by microbial agents. The main steps of this method are the same as in Example 1, except that the actual leather wastewater is used instead of synthetic wastewater for experimental research. At 30°C, the degradation conditions of ammonia nitrogen and COD are as follows: Figure 4 shown. Dilute the leather wastewater by 5 times, at 30°C, the degradation of ammonia nitrogen and COD are as follows: Figure 5 shown.

[0040] The results show that: the original ammonia nitrogen concentration of leather wastewater is 168.6mg / L, and the COD is 3326mg / L. Within 67 hours, the highest removal rate of the microbial agent to the ammonia nitrogen concentration is close to 50%, indicating that the microbial agent can achieve the highest removal rate in the actual leather wastewater. Effective degradatio...

Embodiment 3

[0041] Example 3 Treatment of high-concentration leather wastewater with high-efficiency and enhanced bio-activated carbon technology with microbial agents

[0042] Example utilizes Figure 6 The method of water treatment in the experimental device is as follows: the microbial bacterial liquid is added to the biological activated carbon filter 3, and at the same time, the air compressor 4 is used to aerate under the activated carbon in the biological activated carbon filter 3 to realize the rapid loading of activated carbon by the microbial bacterial agent. The dissolved oxygen (DO) is 2-6mg / L, the temperature is 25-30°C, and after 10-16 hours of aerated film-hanging culture, the aerobic biological activated carbon filter is successfully constructed.

[0043] The leather waste water to be treated is placed in the water tank 1, pumped into the bioactive carbon filter 3 by the water inlet pump 2, and at the same time, the bioactive carbon filter 3 is continuously aerated to keep...

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Abstract

The invention discloses a microorganism bacterium agent and a preparation method thereof, and relates to a composite bacterium agent and a preparation method thereof. The composite bacterium agent can be used for efficiently removing a large amount of COD (chemical oxygen demand), ammonia and nitrogen in leather wastewater. The composite bacterium agent is prepared from mycobacterium, microbacterium, acinetobacter, pichia pastoris, lactobacillus, klebsiella oxytoca and a liquid culture medium. The preparation method comprises the following steps of 1, activating the bacteria strains of raw materials; 2, enriching and culturing; 3, mixing the bacterium strains according to a weight ratio, and adding into the liquid culture medium, so as to obtain the composite bacterium agent. The composite bacterium agent has the advantages that the biological activity is high, the growth speed is high, the leather wastewater can be treated under the low temperature condition, the removal rate of organic matter can be obviously increased, the contents of COD, ammonia and nitrogen in the leather wastewater are effectively decreased, and the effect is obvious.

Description

technical field [0001] The invention relates to a composite microbial agent for leather wastewater treatment and a preparation method thereof. Background technique [0002] According to statistics, there are currently more than 20,000 large, medium and small tanneries in my country, with an annual wastewater discharge of 80-120 million tons, accounting for about 0.3% of the total industrial wastewater in the country. The wastewater produced by the leather industry has a large amount of water, strong volatility, and complex components, containing a large amount of suspended solids, organic matter, ammonia nitrogen, and toxic substances, etc., usually manifested as high concentrations of S 2- and Cr 3+ , The COD concentration of the comprehensive wastewater is 1000-4000mg / L, or even higher, and the high concentration of ammonia nitrogen compounds has poor biodegradability and contains a large amount of neutral salts such as chlorides and sulfates. [0003] The existing leath...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/16C02F3/02C02F3/34C12R1/32C12R1/01C12R1/84C12R1/22C02F103/24
CPCC12N1/16C12N1/20C02F3/02C02F3/34C02F2103/24Y02W10/10
Inventor 金京华刘丹程言君张骥
Owner 北京市科学技术研究院资源环境研究所
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