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Composite microbial inoculum for improving settling performance of activated sludge and manufacture method of composite microbial inoculum

A technology of compound bacterial agent and sedimentation performance, applied in the direction of microorganism-based methods, chemical instruments and methods, biochemical equipment and methods, etc., can solve the problems of neurotoxicity, carcinogenicity, secondary pollution, mutagenesis, etc., and achieve improved sedimentation performance , Strong flocculation ability and low price

Inactive Publication Date: 2012-04-04
JIANGSU SHANGDA WATER AFFAIR
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] ①Inorganic salts, such as aluminum salts and iron salts, the treatment effect is not ideal
② Although the polymer treatment effect is good, but the amount is large, causing secondary pollution to the environment
③Organic synthetic polymers, such as polyacrylamide and its derivatives, have the advantages of less dosage and fast flocculation, but the residues are not easy to be biodegraded, and its monomers have strong neurotoxicity and carcinogenicity. Teratogenic and mutagenic effects, causing secondary pollution
The advantage of this production method is: according to the characteristics of different strains, different culture media and fermentation conditions can be selected to achieve the optimal production of various strains, and the composition ratio of the final bacterial preparation is easy to control; but this method also has certain limitations. Insufficient, such as: the required equipment and other production factors are many, and the activity status of various bacteria in the mixed bacterial agent is different. big change etc.

Method used

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  • Composite microbial inoculum for improving settling performance of activated sludge and manufacture method of composite microbial inoculum
  • Composite microbial inoculum for improving settling performance of activated sludge and manufacture method of composite microbial inoculum
  • Composite microbial inoculum for improving settling performance of activated sludge and manufacture method of composite microbial inoculum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1 improves the composite bacterial agent of activated sludge settling performance

[0029] A composite bacterial agent for improving the settling performance of activated sludge, consisting of Aspergillus sojae CGMCC 3.661, Rhodococcus erythropolis ACCC 02579 and Pseudomonas aeruginosa CGMCC No.1.239; wherein Aspergillus sojae 20-30%, Pseudomonas aeruginosa 40-60%, Rhodococcus erythropolis 20-30%.

Embodiment 2

[0030] The compound microbial agent that the optimization of embodiment 2 improves the settling performance of activated sludge

[0031] The optimal value of the content of the three bacteria: 25% of Aspergillus sojae; 50% of Pseudomonas aeruginosa; 25% of Rhodococcus erythroping. Embodiment 3 improves the preparation method of the composite bacterial agent of activated sludge settling performance

Embodiment 3

[0031] The optimal value of the content of the three bacteria: 25% of Aspergillus sojae; 50% of Pseudomonas aeruginosa; 25% of Rhodococcus erythroping. Embodiment 3 improves the preparation method of the composite bacterial agent of activated sludge settling performance

[0032] 1. Activation of strains

[0033] (1) Activation of Aspergillus sojae (Aspergillus sojae) CGMCC 3.661: Take out the strains stored in the refrigerator at -80°C, melt them on ice, inoculate them on the plate medium by aseptic operation, and culture them at 30°C for 24-36 hours. When a single colony appears and the size is suitable for picking and inoculation, seal it with a parafilm and store it in a 4°C refrigerator for later use. Czapek's plate medium: sucrose 30g·L -1 , agar powder 15g·L -1 、NaNO 3 3g·L -1 , MgSO 4 ·7H 2 O 0.5g·L -1 , KCl 0.5g·L -1 , FeSO 4 4H 2 O 0.01g·L -1 、K 2 HPO 4 1g·L -11. After dissolving with deionized water, adjust the pH to 6.5, sterilize at 121°C for 25 minu...

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Abstract

The invention discloses composite microbial inoculum for improving the settling performance of activated sludge, which consists of Aspergillussojae CGMCC3.661, Rhodococcuserythropolis ACCC02579 and Pseudomonasaeruginosa CGMCCNo.1.239. The composite microbial inoculum has the advantages that the flocculating capability is high, no public hazard exists, a good synergistic effect with original microbes of activated sludge can be generated, the settling performance of the activated sludge is improved, the activated sludge expansion is effectively prevented, and the secondary settling pool treatment efficiency is improved. In addition, the culture medium designed in the composite microbial inoculum has low price and meets the industrial high-intensity fermentation requirement of the composite microbial inoculum, the designed fermentation process control condition reaches the high-intensity production requirement of the composite microbial inoculum (the dry weight of thalli in fermentation liquid reaches 30 g.L<-1> or higher after the fermentation is completed), the investment saving of production factors such as equipment and the like is realized, and the basis is laid for the scale production and the application of the composite microbial inoculum.

Description

technical field [0001] The invention relates to a composite microbial agent for improving the settling performance of activated sludge and a preparation method thereof, belonging to the fields of fermentation and sewage treatment. Background technique [0002] Sewage treatment generally uses a secondary treatment process that combines physical treatment and biological treatment. The biological treatment mainly uses activated sludge to degrade the pollutants in the sewage. The type and activity of microorganisms in activated sludge are important factors affecting the effect of sewage treatment. In addition, the settling performance of activated sludge in the secondary settling tank directly affects the quality of the effluent and the operating efficiency of the sewage treatment system; In the sludge tank, flocculants are generally added to concentrate and settle, and then press to remove water. Currently widely used flocculants include the following categories: [0003] ①In...

Claims

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Application Information

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IPC IPC(8): C12N1/00C12N1/14C12N1/20C02F3/34C12R1/01C12R1/66C12R1/385
Inventor 倪丹荆政徐卫东
Owner JIANGSU SHANGDA WATER AFFAIR
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