Pseudomonas XD-3 and application thereof, and microbial flocculant

A microbial flocculant and pseudomonas technology, applied in the direction of microorganisms, microorganisms, and methods based on microorganisms, can solve the problems of inability to achieve mud-water separation, achieve optimized mud-water separation effects, good application prospects, and improve mud-water separation effects Effect

Inactive Publication Date: 2018-12-21
CHONGQING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The object of the present invention is to provide a kind of Pseudomonas and its application, and also provide a kind of microbial flocculant at the same time, aiming at solving the inability of microorganisms with aerobic denitrification ability in the prior art to treat nitrogen-containing wastewater. Technical Problems in Achieving Effective Slurry-Water Separation

Method used

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  • Pseudomonas XD-3 and application thereof, and microbial flocculant
  • Pseudomonas XD-3 and application thereof, and microbial flocculant
  • Pseudomonas XD-3 and application thereof, and microbial flocculant

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1: Isolation and Identification of Pseudomonas XD-3

[0041] 1. Isolation and purification of Pseudomonas XD-3

[0042] The activated sludge used in this example is taken from the reflux well outside the aeration tank of Chongqing Jiguanshi Sewage Plant, and the screening of Pseudomonas XD-3 adopts the plate marking method, and the liquid culture condition is 25 ° C ~ 35 ° C 1. The rotating speed of the shaker is 90rpm-150rpm, and the culture condition of the solid plate is sterile culture at 30°C-35°C in a biochemical incubator. Concrete separation and purification are carried out according to the following steps:

[0043] 1) Preliminary screening and enrichment of strains: Add a certain amount of glass balls used to break up activated sludge into a triangular flask containing 95mL of sterile water, after sterilization, take 5mL from Jiguanshi, Chongqing City The activated sludge from the reflux well outside the aeration tank of the sewage plant was inoculate...

Embodiment 2

[0055] Example two: Pseudomonas XD-3 on NO in wastewater 3 - -Degradation performance of N, TN and COD

[0056] Inoculate Pseudomonas strain XD-3 with KNO 3 as nitrogen source, C 6 h 5 Na 3 o 7 2H 2 In the wastewater where O is the carbon source, the detection of strains on NO in wastewater 3 - - Degradation of N, TN and COD and NO 2 - The accumulation of -N. Among them, COD, TN and NO in wastewater 3 -The initial concentrations of -N were 1898mg / L, 53.9mg / L, 49.3mg / L respectively. After inoculation, it was placed in a 30°C, 120rpm constant temperature shaking incubator for continuous aerobic culture for 72 hours, and the cultured wastewater was taken regularly and centrifuged to measure the COD and OD in the supernatant. 600 (the absorbance value of the solution at 600nm wavelength, indicating the cell density) and TN, NO 3 - -N and NO 2 - -N content.

[0057] Specifically, as figure 2 with image 3 As shown, it shows the application results of Pseudomon...

Embodiment 3

[0063] Example 3. Analysis of the flocculation ability of the flocculation active substance produced by Pseudomonas XD-3

[0064] Experimental results such as Figure 4 As shown, in this example, the fermentation medium was used as the medium to cultivate Pseudomonas XD-3, and the flocculation ability of the flocculation active substance produced by the strain was investigated. Specifically, this example uses the flocculation activity distribution experiment to analyze the flocculation ability of different active substances produced by Pseudomonas XD-3. Active substances: fermentation stock solution, the sterilized supernatant obtained after the fermentation stock solution was centrifuged, and the bacterial cell suspension of Pseudomonas XD-3 cells obtained after the fermentation stock solution was centrifuged. Flocculation ability. More specifically, the experimental procedure is as follows:

[0065] Flocculation activity distribution test: Pseudomonas strain XD-3 was inoc...

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Abstract

The invention belongs to the field of applied and environmental microbiology, and particularly relates to pseudomonas and application thereof, and a microbial flocculant. The pseudomonas XD-3 is namedas Pseudomonas sp., and has been collected into General Microbiology Committee of China Committee for Culture Collection of Microorganisms; the collection number is CGMCC No.15858. The pseudomonas XD-3 has the advantages that the pseudomonas XD-3 can complete high-efficiency degrading of NO3-N and synchronous removal of organic carbon in wastewater under the aerobic condition; in the aerobic denitrification process, the pseudomonas XD-3 can be used for preparing the microbial flocculant; when the pseudomonas XD-3 is applied to the treatment of nitrogen-containing organic wastewater, the pseudomonas XD-3 can be used as a flocculant for accelerating sludge and water separating; the larger application potential and good application prospect are realized in the treatment of nitrogen-containing organic wastewater.

Description

technical field [0001] The invention belongs to the technical field of applied environmental microorganisms, and in particular relates to a pseudomonas XD-3, its application and a microbial flocculant. Background technique [0002] Nitrogen pollution is one of the most harmful and most common types of pollution in water pollution. It has a wide range of sources. Modern industrial sewage, farmland fertilization and drainage, and urban domestic sewage are the main sources of nitrogen pollution in water bodies. Therefore, in recent years, many water treatment researchers have devoted themselves to exploring an economical, energy-saving, efficient and feasible denitrification treatment technology. The practice of modern sewage denitrification technology shows that biological denitrification is one of the most effective, feasible, economical and energy-saving denitrification technologies. Traditional biological denitrification is mainly accomplished by synergistic nitrification ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P1/04C02F3/34C12R1/38C02F101/38
CPCC02F3/34C02F2101/38C12P1/04C12N1/205C12R2001/38
Inventor 赵彬安强陈宇航冉小川但巧
Owner CHONGQING UNIV
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