Detection vessel for rapidly detecting moulds and yeasts and preparation method

A yeast and mold technology, applied in biochemical equipment and methods, microbial measurement/inspection, etc., can solve problems affecting the interpretation of test results, coating and covering film bubbles, improper manual operation, etc., to achieve three-dimensional growth, Strong adsorption and low cost effect

Inactive Publication Date: 2016-04-13
贵州勤邦食品安全科学技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Hubei University of Technology published an invention patent "Preparation and Application of Rapid Detection of Coliform Bacteria in Food and Tableware". Put the filter paper into a sterile plastic mold bag and assemble the test piece. The target bacteria are in the assembled bag. Disadvantages of diffusion growth, fuzzy colony edges, and affecting the interpretation of test results
3M Innovation Co., Ltd. applied for an invention patent "Method and culture device for detecting yeast and mold" 201480007414.5. The culture device needs to coat the sample to be tested, and the test result can be judged within a culture time of no more than 72 hours. The disadvantage is that improper manual operation may cause air bubbles in the coating and cover film.
3M Innovation Co., Ltd. has also applied for an invention patent "Device for Rapid Detection of Microorganisms" 201180032280.9. The use of this device depends on the coating and covering of the sample to be tested, and introduces errors in interpretation results caused by improper human operation. possible

Method used

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  • Detection vessel for rapidly detecting moulds and yeasts and preparation method
  • Detection vessel for rapidly detecting moulds and yeasts and preparation method
  • Detection vessel for rapidly detecting moulds and yeasts and preparation method

Examples

Experimental program
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Effect test

Embodiment 1

[0027] A detection dish for rapid detection of mold and yeast, including a sterile petri dish and a test piece, the test piece is spread on the bottom of the sterile petri dish, and the characteristic chromogenic liquid culture medium of mold and yeast is attached to the test piece;

[0028] Among them, the composition of the medium, per 100g of distilled water contains: tryptone 0.8g, yeast powder 0.4g, glucose 1g, dipotassium hydrogen phosphate 0.05g, sodium chloride 0.05g, potassium dihydrogen phosphate 0.2g, magnesium sulfate 0.2g, Bengal red 0.04g, chloramphenicol 0.1g, N,N-dimethylformamide 2g, enzymatic hydrolysis substrate mixture 0.015g. The enzymatic hydrolysis substrate mixture is obtained by dissolving 5-bromo-4-chloro-3-indolyl-phosphate and NBT in N,N-dimethylformamide at a ratio of 2:1. The test piece is white medium-speed filter paper with a diameter of 70 mm, and each piece of filter paper is treated with 0.05 Mol / L Triton-Tris buffered saline solution, and th...

Embodiment 2

[0036] A detection dish for rapid detection of mold and yeast, including a sterile petri dish and a test piece, the test piece is spread on the bottom of the sterile petri dish, and the characteristic chromogenic liquid culture medium of mold and yeast is attached to the test piece;

[0037] Among them, the composition of the medium, per 100g of distilled water contains: tryptone 1.2g, yeast powder 0.6g, glucose 3g, dipotassium hydrogen phosphate 0.08g, sodium chloride 0.1g, potassium dihydrogen phosphate 0.3g, magnesium sulfate 0.3g, Bengal red 0.06g, chloramphenicol 0.2g, N,N-dimethylformamide 3g, enzymatic hydrolysis substrate mixture 0.02g. The enzymatic hydrolysis substrate mixture is obtained by dissolving 5-bromo-4-chloro-3-indolyl-phosphate and NBT in N,N-dimethylformamide at a ratio of 1:1. The test piece is white medium-speed filter paper with a diameter of 70mm, and each piece of filter paper is treated with 0.05Mol / L Triton-Tris buffered saline solution, and the am...

Embodiment 3

[0045] A detection dish for rapid detection of mold and yeast, including a sterile petri dish and a test piece, the test piece is spread on the bottom of the sterile petri dish, and the characteristic chromogenic liquid culture medium of mold and yeast is attached to the test piece;

[0046] Among them, the composition of the medium, per 100g of distilled water contains: tryptone 2.0g, yeast powder 0.8g, glucose 4g, dipotassium hydrogen phosphate 0.1g, sodium chloride 0.2g, potassium dihydrogen phosphate 0.4g, magnesium sulfate 0.4g, Bengal red 0.1g, chloramphenicol 0.3g, N,N-dimethylformamide 4g, enzymatic hydrolysis substrate mixture 0.04g. The enzymatic hydrolysis substrate mixture is obtained by dissolving 5-bromo-4-chloro-3-indolyl-phosphate and NBT in N,N-dimethylformamide at a ratio of 1:2. The test piece is a white medium-speed filter paper with a diameter of 70mm, and each piece of filter paper is treated with 0.05Mol / L Triton-Tris buffered saline solution, and the am...

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Abstract

The invention provides a detection vessel capable of rapidly culturing and detecting moulds and yeasts at the same time. A biochemical reaction characteristic of microorganisms is utilized, types and reaction conditions of endoenzymes of the microorganisms are determined as main bases for microorganism classification and identification, a microorganism specific enzyme chromogenic substrate is added into an isolation medium or a selective medium, and when target bacteria grow on the medium, the chromogenic substrate can be degraded by specific enzymes and metabolites in special colors can be generated to realize specific colors or forms of colonies, thereby implementing culturing and detection of the microorganisms in a sample. The detection vessel has the advantages of convenience in operation, low cost, labor saving, simple judgment result, reliable detection result, short detection time and the like, and can play an important role in microorganism detection, and accuracy of a detection result reaches 90 percent or over 90 percent of a national standard detection method.

Description

technical field [0001] The technical field to which the patent of the present invention belongs is microbiological detection, and specifically relates to a detection dish for rapid detection of mold and yeast and a preparation method thereof. Background technique [0002] Mold and yeast widely exist in the air, water and soil. Most of them are harmless to the human body, but some molds are harmful to the human body. Mold can cause mildew in food and stimulate the human digestive tract and stomach. Cause damage to the liver, cause food poisoning, and endanger life safety. The current detection technology mainly includes GB4789.15-2010 national food safety standard, food microbiology detection - mold and yeast counting, this method is a plate method, with the characteristics of accurate and reliable detection results, it is a classic microbial detection and identification method, However, this method is time-consuming, needs to prepare the culture medium in advance, and the o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/04
CPCC12Q1/04C12Q1/045
Inventor 杨永菊陈娇金帮明王亚娟杨兴伟侯璐伟陆苇刘红罗贵昆李平冯才伟何进党娟
Owner 贵州勤邦食品安全科学技术有限公司
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