Hypsizygus marmoreus strain and application thereof

A technology of shimeji mushroom and strain, which is applied in the field of shimeji mushroom Finc-B-3 strain, can solve the problems of long cultivation period of strains and cannot meet market demand, etc., and achieves excellent morphological characteristics, short cultivation and cultivation period, and yield per unit. high effect

Active Publication Date: 2015-12-23
青岛丰科生物科技有限公司 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The invention provides a true jiji mushroom strain and its application, which is used to solve the problem that the current strains have a long cultivation cycle and cannot meet the market demand

Method used

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  • Hypsizygus marmoreus strain and application thereof
  • Hypsizygus marmoreus strain and application thereof
  • Hypsizygus marmoreus strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1 hybridization obtains Finc-B-3

[0034] The strain Finc-B-3 was obtained by hybrid breeding method.

[0035] Specific steps are as follows:

[0036] 1) Select parental strains: select bacterial strain TNN-12 and bacterial strain F-FG as parents, and the fruiting bodies of these two parents can be purchased in the market;

[0037] 2) Prepare mother seed culture medium, original seed culture medium and cultivar culture medium: the formulations of each culture medium have been given, and will not be repeated here;

[0038] 3) 300 heterozygotes were obtained by conventional cross-breeding methods;

[0039] 4) Cultivation and screening: The obtained heterozygotes are cultivated according to conventional methods, and through systematic screening, a target strain is obtained from 300 heterozygotes, and the strain is named Finc-B-3. Its cultivation and The cultivation period is short, the mushroom shape is good, and the consistency between bottles is high.

Embodiment 2

[0040] Embodiment 2 Antagonism experiment

[0041] In the present invention, the mushroom strain Finc-B-3 and two parental strains TNN-12 and F-FG are used as test materials, and the medium used is PDA medium.

[0042] Finc-B-3 and TNN-12, Finc-B-3 and F-FG were inoculated and cultured on the plate medium respectively, the inoculation points were 3cm apart, and cultured at 23°C for 33 days. Please refer to Table 1 for the results of this experiment.

[0043] Table 1 Antagonism test among different strains of jijiji mushrooms

[0044] Variety

TNN-12

F-FG

Finc-B-3

+

+

[0045] Note: "+" indicates antagonism.

[0046] Such as Figure 1-4 As shown, the antagonistic lines between Finc-B-3 and TNN-12, Finc-B-3 and F-FG were obvious on the front and back of the junction of the confrontation culture colonies, indicating that Finc-B-3 was related to TNN-12, F-FG -FG different Shimeji mushroom strains.

Embodiment 3

[0047] Embodiment 3 cultivation experiment

[0048] The present invention's jiji mushroom strain Finc-B-3, the most widely planted TNN-12 and jiji mushroom strain Finc-B-1 among the existing jiji mushroom varieties (disclosed in Chinese patent CN103004464A, the strain was published in August 2012 It was preserved in the China Typical Culture Collection Center on the 13th, and the preservation number is CCTCCM2012308) to carry out the optimization experiment of the cultivation cycle and the cultivation cycle respectively, each kind of 80 bottles of each cultivation, the cultivation cycle, the cultivation cycle and the yield per unit area were compared, and the truth of the present invention was summarized. Advantages and characteristics of Jiji mushroom strain Finc-B-3.

[0049] The cultivation conditions that present embodiment adopts are as follows:

[0050] See cultivar culture medium for culture medium, no more details.

[0051] Cultivation bottle volume: 850cc.

[0052]...

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Abstract

The invention belongs to the technical field of edible mushrooms and particularly relates to a hypsizygus marmoreus Finc-B-3 strain and application thereof. The hypsizygus marmoreus strain provided by the invention is named as Finc-B-3 and is obtained by taking a strain TNN-12 and a strain F-FG as parents to be hybridized through systematic selection, belongs to hypsizygus marmoreus and is preserved in Institute of Microbiology Chinese Academy of Sciences, wherein the address is No.3, No.1 Yard, West Beichen Road, Chaoyang District, Beijing, the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.11204 and the preservation date is 20150813. Compared with the prior art, the hypsizygus marmoreus Finc-B-3 strain provided by the invention has short culture and cultivation periods, so that the production efficiency of factories is greatly improved; the unit yield is high, the yields of bottles are similar, and topographic characteristics are good, so that sub-packaging and sales of products are very easy; and the Finc-B-3 has good comprehensive performances and a wide market prospect.

Description

technical field [0001] The invention belongs to the technical field of edible fungi, and in particular relates to jiji mushroom Finc-B-3 strain and application thereof. Background technique [0002] Hypsizygus marmoreus (Latin Hypsizygus marmoreus) belongs to Basidiomycotina, Phyllomycetes, Agaricaceae, Agaricaceae, Liphyllum family, and Amygium genus. Because it has a unique crab flavor, it is also called crab-flavored mushroom. At present, there are mainly two types of Shiji mushrooms produced in factories: brown and pure white. Pure white Shiji mushrooms are also called white jade mushrooms or jade dragon mushrooms. [0003] True jiji mushrooms are rich in vitamins and 17 kinds of amino acids, among which the content of lysine and arginine is higher than that of common mushrooms, which can help teenagers improve intelligence, fight cancer and lower cholesterol. In particular, the extract of the fruiting body (the part above the culture medium) has various physiologicall...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H15/00A01G1/04
Inventor 刘玉吴慧敏张文宏
Owner 青岛丰科生物科技有限公司
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