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EDDHA or ethylenediamine-N,N′-bis(2-hydroxyphenylacetic acid) is a chelating agent. Like EDTA, it binds metal ions as a hexadentate ligand, using two amines, two phenolate centers, and two carboxylates as the six binding sites. The complexes are typically anionic. The ligand itself is a white, water soluble powder. Both the free ligand and its tetraanionic chelating agent are abbreviated EDDHA. In contrast to EDDHA, most related aminopolycarboxylic acid chelating agents feature tertiary amines and few have phenolate groups.
A method for determining the ortho value iron content in EDDHA-Fe chelate by HPLC
This invention discloses an HPLC method for determining the ortho-O iron content in EDDHA-Fe chelates, comprising the following steps: S1. Sample pretreatment: S11. The sample is first extracted with an aqueous solution, followed by solid-liquid separation; S12. The liquid phase is purified by passing it through a strong acid cation exchange resin to obtain a purified solution; S13. EDTA salt is added to obtain a test solution; S2. HPLC detection: A C18 reversed-phase column is used, with gradient elution using mobile phase A and mobile phase B; mobile phase A is a 0.05-0.15% (w / w) aqueous solution of phosphoric acid with a pH of 2.5-3.0; mobile phase B is methanol. This invention, through optimized chromatographic conditions and innovative sample pretreatment, establishes a high-resolution, interference-resistant method for O-O detection, solving the problems of insufficient resolution, interference ion influence, and inaccurate quantification in existing methods.
Owner:ZHENGZHOU RUIPU BIOLOGICAL ENG CO LTD