This invention belongs to the field of
plant genetic engineering technology, specifically relating to a
tobacco nicotine synthesis regulatory
gene NtbHLH25 and its encoded
tobacco nicotine synthesis regulatory
protein NtbHLH25, and further disclosing its applications. This invention cloned a novel
transcription factor gene, NtbHLH25, from cultivated tobacco and studied its role in tobacco synthesis.
Nicotine detection in genetic material showed that lines with reduced NtbHLH25
gene expression had significantly lower
nicotine content in tobacco leaves. The
nicotine synthesis regulatory gene NtbHLH25 and its encoded
protein NtbHLH25 identified in this invention are associated with
nicotine accumulation in tobacco, providing new genetic targets and technical means for the regulation and application of
tobacco nicotine content, and have significant application value in tobacco nicotine regulation.