5results about How to "Lower nicotine levels" patented technology

The invention relates to the technical field of fertilizer preparation, in particular to traditional Chinese medicine residue and mushroom dreg compound fermented fertilizer as well as a preparation method and application thereof. The preparation method of the traditional Chinese medicine residue and fungus residue compound fermented fertilizer comprises the following steps: (1) crushing pleurotus eryngii fungus residues, perilla leaf traditional Chinese medicine residues and cow dung, and mixing to obtain a material 1; and (2) adjusting the carbon nitrogen ratio and the water content of the material 1, and then fermenting. The fermented fertilizer provided by the invention can significantly influence the growth of potted tobacco, soil characteristics and microbial communities, and provides a theoretical basis and practical guidance for optimizing tobacco cultivation and fungus residue utilization.

This invention discloses Aspergillus terreus Aspergillus terreus Applications for reducing nicotine content. Specifically, these applications include Aspergillus terreus. Aspergillus terreus This invention relates to the method for reducing nicotine content in tobacco raw materials, tobacco waste, or tobacco processing byproducts. The method is characterized by high degradation efficiency, mild conditions, and environmental friendliness, providing a new and effective tool for biologically reducing nicotine content in tobacco leaves. It shows promising application prospects in improving tobacco quality and reducing harmful substances and tar content.

This invention belongs to the field of plant genetic engineering technology, specifically relating to a tobacco nicotine synthesis regulatory gene NtbHLH25 and its encoded tobacco nicotine synthesis regulatory protein NtbHLH25, and further disclosing its applications. This invention cloned a novel transcription factor gene, NtbHLH25, from cultivated tobacco and studied its role in tobacco synthesis. Nicotine detection in genetic material showed that lines with reduced NtbHLH25 gene expression had significantly lower nicotine content in tobacco leaves. The nicotine synthesis regulatory gene NtbHLH25 and its encoded protein NtbHLH25 identified in this invention are associated with nicotine accumulation in tobacco, providing new genetic targets and technical means for the regulation and application of tobacco nicotine content, and have significant application value in tobacco nicotine regulation.

The present invention discloses a method for rapidly obtaining a cultivated tobacco nicotine conversion strain and its application. The method for rapidly obtaining a cultivated tobacco nicotine conversion strain is to simultaneously knock out the genes related to tobacco nicotine conversion by using the CRISPR / Cas9 system, the genes being the NtNCP1 and NtNCP2 genes, the NtNCP1 gene sequence being shown in SEQ ID No.1, and the NtNCP2 gene sequence being shown in SEQ ID No.3. The present application uses the CRISPR / Cas9-mediated gene editing technology to simultaneously knock out the tobacco NtNCP1 and NtNCP2 genes to obtain an editing material with reduced nicotine content and increased nicotine reduction content, which provides genetic materials and theoretical basis for the functional study of tobacco nicotine conversion genes and the directional improvement of new tobacco varieties for regulating nicotine content.

The invention belongs to the technical field of plant genetic engineering, particularly relates to a tobacco nicotine synthesis regulation gene NtbHLH25 and a tobacco nicotine synthesis regulation protein NtbHLH25 encoded by the same, and further discloses application of the tobacco nicotine synthesis regulation gene NtbHLH25 and the tobacco nicotine synthesis regulation protein NtbHLH25. According to the invention, a new transcription factor gene NtbHLH25 is cloned from cultivated tobacco, the effect of the gene in tobacco synthesis is studied, and detection on nicotine of genetic materials shows that the nicotine content in strains and tobacco leaves with reduced NtbHLH25 gene expression quantity is obviously reduced. The gene NtbHLH25 for regulating and controlling nicotine synthesis and the encoded protein NtbHLH25 identified by the invention are related to nicotine accumulation in tobacco, provide a new genetic target and technical means for regulation and application of tobacco nicotine content, and have important application value in tobacco nicotine regulation and control.