The invention relates to a pesticide combination for prevention and treatment of aphis gossypii glover and a preparation method thereof, and belongs to the technical field of pesticide. The pesticide combination comprises a spraying agent and a root-irrigation agent which are combined for use. The spraying agent is prepared from aloes, ailanthus altissima leaves, safflower, quisqualis indica, dichroa febrifuga lour and sucrose fatty acid ester. The root-irrigation agent is prepared from diethyl aminoethyl hexanoate, garlic oil, KH2PO4, glucoheptonic acid, phosphatidyl ethanolamine, polyethylene glycol 200 and herb residues obtained after the spraying agent is extracted. According to the pesticide combination, a Chinese traditional medicine extracting solution is used for killing the aphis gossypii glover with poison, the prevention and control effect is good, environmental friendliness is achieved, low toxicity is achieved, the quantity of residues is small, pesticide resistance of the aphis gossypii glover is not prone to be achieved, and thus the pesticide combination is ideal green pesticide; the spraying agent is combined with the root-irrigation agent for use, nutrients needed by crops can be supplemented while the aphis gossypii glover is killed with poison, growth and development of the crops are promoted, and hence the yield is increased; the spraying agent can dissolve honeydew defecated by the aphis gossypii glover, mold breeding is reduced, the influence on the crops by the aphis gossypii glover is eliminated completely, and the growth vigor of the crops is recovered.