28 results about "Erythrocyte hemolysis" patented technology

The invention discloses design of an antibacterial peptide and application of the antibacterial peptide in bacterial infection, belongs to the technical field of biomedical polypeptides, and particularly relates to the antibacterial peptide designed through an amino acid replacement strategy and application of the antibacterial peptide in preparation of drugs for preventing or treating bacterial infection related diseases. The polypeptide designed in the invention has the advantages of short amino acid sequence, high efficiency and rapidness in preparation, strong antibacterial activity, wide antibacterial spectrum and weak red blood cell hemolysis activity, can be used for preventing and treating diseases caused by various microbial infections, and has a good treatment effect on diseases such as pneumonia caused by skin wound infection, sepsis and bacterial infection.

The present application relates to the technical field of vaccine production, in particular to a preparation method of a Sapindus saponin veterinary vaccine adjuvant, comprising: determining the limit concentration of Sapindus saponin with a hemolysis rate lower than 5% and preparing the vaccine adjuvant; determining the limit concentration of Sapindus saponin with a hemolysis rate lower than 5% against red blood cells by spectrophotometry; preparing the vaccine adjuvant by dialysis; and the vaccine adjuvant contains soybean lecithin and cholesterol in a weight ratio of 1:1. The present application first determines the safe use concentration of Sapindus saponin through a standard hemolysis experiment, ensures that the hemolysis rate of the prepared adjuvant meets the safety requirements, and avoids adverse reactions after injection; at the same time, the present application uses soybean lecithin and cholesterol as raw materials, and prepares the nano-liposome adjuvant embedding Sapindus saponin by dialysis, which does not require complex process operation, has low preparation cost, and has safety and practicability, and can effectively assist the vaccine to induce the body to produce stronger immune response and improve the protection effect of the vaccine.

The invention belongs to the technical field of biology, and particularly relates to a method for establishing a progesterone non-gene anti-oxidative stress model. The method comprises the following steps: centrifuging in-vitro blood; centrifuging and then collecting lower-layer red blood cell precipitate to obtain red blood cells; the method comprises the following steps: taking red blood cells, preparing a red blood cell suspension with a volume percentage of 5% by using PBS, adding progesterone and 30mmol / L-80mmol / L of azobis (isobutylamidine hydrochloride) into the red blood cell suspension, and finally obtaining a red blood cell suspension containing the progesterone and the azobis (isobutylamidine hydrochloride); heating and centrifuging; centrifuging, taking a supernatant, measuring the absorbance of 535-545 nm, and recording the absorbance as A1 '; taking red blood cells, and preparing red blood cell suspension which only contains 5 percent by volume of the red blood cells by using PBS (Phosphate Buffer Solution); performing centrifugation; the method comprises the following steps: taking a progesterone sample as a raw material, centrifuging, taking a supernatant, measuring absorbance of 535nm-545nm, recording the absorbance as A2 ', and calculating a red blood cell hemolysis rate according to a percentage value of A1' and A2 'to obtain the progesterone non-gene anti-oxidative stress mode.The method has the advantages that the red blood cells are used for the first time, and the progesterone non-gene anti-oxidative stress model is established.

The present invention relates to a fluorescent probe based on a lanthanide metal organic framework, a preparation method and an application, and belongs to the field of nanomaterial technology. The fluorescent probe provided by the present invention is obtained by covalently cross-linking a lanthanide metal organic framework with a neutrophil elastin binding peptide and a P-selectin binding peptide, and the surface of the lanthanide metal organic framework is modified with a tetracyclic antibiotic and arginine. The fluorescent probe prepared by the present invention specifically targets activated platelets and neutrophils, does not affect cell survival rate, does not cause hemolysis of red blood cells, and can be applied to the preparation of diagnostic products for thrombotic diseases. The use of a fluorescent probe loaded with urokinase achieves stable and efficient delivery of urokinase, inhibits platelet aggregation, slows down blood clot contraction, has a good thrombolytic effect, and has the potential to be used in the preparation of drugs for the treatment of thrombotic diseases. The materials required for the present invention are easy to obtain, the preparation method is simple, and a new method is provided for the diagnosis and treatment of thrombotic diseases.

This invention discloses crystals of nitrogen-containing spiro compounds, methods for producing the same, and uses thereof. This invention provides a type I crystal of a nitrogen-containing spiro compound represented by formula A, in which the powder X-ray diffraction pattern displayed at a 2θ angle using Cu-Kα rays has diffraction peaks at positions 5.67±0.20°, 11.37±0.20°, 16.69±0.20°, 17.32±0.20°, and 19.73±0.20°. This invention also provides a type II crystal of a nitrogen-containing spiro compound represented by formula A, in which the powder X-ray diffraction pattern displayed at a 2θ angle using Cu-Kα rays has diffraction peaks at positions 11.32±0.20°, 11.70±0.20°, 11.93±0.20°, 18.03±0.20°, 18.81±0.20°, and 19.17±0.20°. The nitrogen-containing spiro compound crystals of the present invention exhibit good stability and complement factor D inhibitory activity, good inhibitory effect against rabbit erythrocyte hemolysis, high in vivo exposure levels, and high oral bioavailability. JPEG2026513298000037.jpg5469

The invention discloses a 1, 10-split ring type guaiane sesquiterpene lactone quasi-natural product as well as a preparation method and application of the 1, 10-split ring type guaiane sesquiterpene lactone quasi-natural product. The quasi-natural product and the derivative thereof are prepared by taking (R)-Carvone as a raw material through a chemical synthesis method. Experiments show that the 1, 10-split ring type guaiane sesquiterpene lactone compound prepared by the invention has the effect of inhibiting red blood cell hemolysis, can be used as a candidate drug molecule for preventing and / or treating NLRP3 inflammasome related diseases, and has the potential of being developed into related anti-inflammatory drugs.

The invention discloses a blood hemolysis detection kit and a detection method, and the kit comprises a hemoglobin cracking reagent which is used for destroying hemoglobin to release internal iron ions and / or ferrous ions; and the iron ion fluorescent probe is used for detecting the iron ion concentration in combination with fluorescence detection. The invention provides a strategy different from conventional hemolysis detection: the hemolysis degree is indirectly judged by detecting the content of Fe, specifically, the erythrocyte releases free hemoglobin after hemolysis, and the hemoglobin contains specific iron; based on the principle, hemoglobin is destroyed and cracked to release internal Fe and / or Fe, then a self-made iron ion fluorescent probe is adopted, and a fluorescence measurement means is combined, so that the iron ion concentration can be detected, and the hemolysis degree of the blood to be detected is judged, namely the higher the iron ion concentration is, the more serious the hemolysis is.

The invention discloses an image processing method and device for judging suspension red blood cell hemolysis. The method comprises the following steps: acquiring a blood image set; the blood image set comprises a plurality of blood images and corresponding label information; acquiring a blood parameter information set; the blood parameter information set comprises a plurality of blood parameters and corresponding label information; the blood parameters comprise hemoglobin concentration, reticulocyte concentration, indirect bilirubin concentration, lactic dehydrogenase concentration and globin concentration; utilizing the blood image set and the blood parameter information set to construct a blood hemolysis composite detection model; based on the blood hemolysis composite detection model, processing the collected blood image and blood parameters to obtain hemolysis detection result information; the hemolysis detection result information is used for representing erythrocyte hemolysis information of the blood corresponding to the collected blood image and blood parameters.

The invention relates to a blood shearing device and a blood compatibility evaluation method. The blood shearing device comprises an objective table, a fixing support, a supporting beam, a stator, a rotor and a driving source. The device can simultaneously adjust the roughness and wettability of the material surface, breaks through the limitation that only a single factor can be researched in the prior art, and provides a more comprehensive platform for systematically evaluating the blood compatibility; in the experimental method, red blood cell hemolysis is detected, and the degradation condition of plasma protein is evaluated synchronously, so that the overall injury mode of blood components under the action of an artificial surface can be reflected more truly. Experimental basis can be provided for material selection and surface optimization of blood contact type medical instruments such as an artificial heart pump, an ECMO and a hemodialysis machine, the clinical complication risk can be reduced, and development of artificial organs and blood purification technologies is promoted.

The present invention discloses a metal ruthenium complex with a binuclear structure for resisting drug-resistant bacteria, a preparation method and an application thereof, and relates to the field of antibacterial medicine technology. The structure of the complex is as follows:, which can be used to prepare drugs for resisting drug-resistant bacteria, reducing hemolysis of red blood cells, inhibiting MRSA and / or inhibiting the formation of MRSA biofilms. Since the complex contains metal ions and carries four positive charges, compared with traditional organic small molecules, it enhances its ability to penetrate bacterial cell membranes and retention effect, and the multi-coordinate configuration of the metal complex allows it to be modified with different ligands, thereby making it more biologically active. Experiments have shown that the bis-ruthenium polypyridine complex of the present invention can effectively reduce the hemolytic effect of toxins released by MRSA on red blood cells, and can inhibit the formation of MRSA biofilms.

The invention discloses an anti-drug-resistant bacterium metal ruthenium complex with a dual-core structure as well as a preparation method and application thereof, and relates to the technical field of antibacterial medicines. The structure of the complex is as follows: # imgabs0 #, and the complex can be used for preparing drugs for resisting drug-resistant bacteria, relieving red blood cell hemolysis, inhibiting MRSA and / or inhibiting MRSA biofilm formation. The complex contains metal ions and has four positive charges, compared with traditional small organic molecules, the ability of penetrating through bacterial cell membranes and the retention effect of the complex are enhanced, and due to the multi-coordination configuration of the metal complex, the metal complex can be modified by different ligands, so that the biological activity of the metal complex is higher. Experiments prove that the diruthenium polypyridine complex disclosed by the invention can effectively reduce the hemolysis effect of toxin released by MRSA on red blood cells, and can inhibit the formation of an MRSA biological membrane.

The invention discloses an antibacterial peptide and application thereof as a medicine and a feed additive. The antibacterial peptide is obtained by modifying an amino acid sequence as shown in SEQ ID No: 1. A potential antibacterial peptide sequence is excavated from channel catfish proteome, and a novel antibacterial peptide which has broad-spectrum antibacterial activity and particularly has a remarkable inhibition effect on APEC is obtained through structural modification and optimization. The antibacterial peptide can effectively inhibit the growth of various drug-resistant APEC strains in vitro, shows good safety in cytotoxicity and red blood cell hemolysis experiments, and can be used as a powerful supplement for traditional antibiotic and vaccine prevention and control.

The present invention discloses an anti - enzymolysis antibacterial peptide FRL, its preparation method and application, belonging to the field of bioengineering. The sequence of the antibacterial peptide FRL is shown as SEQ ID No.1. This antibacterial peptide FRL exhibits narrow - spectrum antibacterial activity, can effectively inhibit Gram - negative bacteria, and has the potential to be applied in drugs for treating Gram - negative bacterial infectious diseases. This antibacterial peptide has very low hemolytic activity and eukaryotic cell toxicity. This antibacterial peptide fails to cause 10% erythrocyte hemolysis at a concentration of 128 μmol / L, and the survival rate of mouse macrophage RAW264.7 reaches more than 80%. This antibacterial peptide shows good stability against trypsin, pepsin, chymotrypsin, and proteinase K (0.4 mg / mL; 0.5 h) at pH = 5 and 37°C. In summary, the antibacterial peptide FRL has the development potential to become an alternative to antibiotics.

The invention relates to the field of biological medicine, in particular to a whole blood quality control product which comprises mixed hydroformylated red blood cells, protection liquid and plasma. The preparation method of the hydroformylated red blood cells comprises the following steps: pre-immobilizing red blood cells through non-crosslinked aldehyde, and then mainly immobilizing the red blood cells through crosslinked aldehyde; a quality control standard substance is also added into the whole blood quality control product, and the quality control standard substance is one or more of cholesterol, triglyceride, high-density lipoprotein, low-density lipoprotein, blood sugar and uric acid. Compared with the prior art, the whole blood quality control product has the advantages of low red blood cell hemolysis rate and longer shelf life.

The application provides a preparation method of YjgB family protein and application of the YjgB family protein as a domestication immune agonist vaccine adjuvant; the YjgB family protein is isolated from Bacillus megaterium and has domestication immune activity, and shows a strong natural immune memory induction effect; the protein is relatively stable in nature, is not easily affected by temperature and pH, has a low red blood cell hemolysis rate and no cytotoxicity, and lays a theoretical foundation for practical application; OVA combined with the protein can improve the immune response of the body, has the potential to become a new adjuvant, and lays a solid foundation for future development of a new adjuvant and a double memory vaccine with innate immune memory and adaptive immunity.

The invention relates to an antibacterial peptide extracted from kitchen waste liquid and a preparation method of the antibacterial peptide, and belongs to the technical field of biology. According to the invention, the kitchen waste liquid is used as a raw material, and ultrasonic enzymolysis, microbial fermentation, purification and other processes are adopted to obtain the polypeptide with antibacterial activity; the antibacterial peptide prepared by the invention has broad-spectrum antibacterial activity, can generate inhibiting and killing effects on various pathogens such as bacteria and fungi, is relatively low in red blood cell hemolytic activity, short in synthetic sequence, small in molecular weight and small in chemical synthesis difficulty, can specifically kill pathogenic bacteria in organisms, can well save the large-scale production cost, and is suitable for industrial production. The polypeptide can be used as a polypeptide microbial inhibitor to be applied to the fields of feed, food, skin care products, medicine, medical equipment, animal husbandry, agriculture and the like.

The present application relates to the technical field of vaccine production, in particular to a preparation method of a Sapindus saponin veterinary vaccine adjuvant, comprising: determining the limit concentration of Sapindus saponin with a hemolysis rate lower than 5% and preparing the vaccine adjuvant; determining the limit concentration of Sapindus saponin with a hemolysis rate lower than 5% against red blood cells by spectrophotometry; preparing the vaccine adjuvant by dialysis; and the vaccine adjuvant contains soybean lecithin and cholesterol in a weight ratio of 1:1. The present application first determines the safe use concentration of Sapindus saponin through a standard hemolysis experiment, ensures that the hemolysis rate of the prepared adjuvant meets the safety requirements, and avoids adverse reactions after injection; at the same time, the present application uses soybean lecithin and cholesterol as raw materials, and prepares the nano-liposome adjuvant embedding Sapindus saponin by dialysis, which does not require complex process operation, has low preparation cost, and has safety and practicability, and can effectively assist the vaccine to induce the body to produce stronger immune response and improve the protection effect of the vaccine.

The invention discloses an anti-enzymolysis antibacterial peptide FRL as well as a preparation method and application thereof, and belongs to the field of bioengineering, and the sequence of the antibacterial peptide FRL is as shown in SEQ ID No.1. The antibacterial peptide FRL shows a narrow-spectrum antibacterial effect, can effectively inhibit gram-negative bacteria, and has the potential of being applied to drugs for treating gram-negative bacteria infectious diseases. The antibacterial peptide has very low hemolytic activity and eukaryotic cell toxicity. The antibacterial peptide cannot cause 10% red blood cell hemolysis under the concentration of 128 [mu] mol / L, and the survival rate of mouse macrophages RAW264.7 reaches 80% or above. The antibacterial peptide can be used for treating trypsin, pepsin, chymotrypsin and protease K (0.4 mg / mL) under the conditions that the pH is equal to 5 and the temperature is 37 DEG C; 0.5 h), good stability is shown. In conclusion, the antibacterial peptide FRL has the development potential of becoming an antibiotic substitute.

The invention discloses an image processing method and device for judging suspension red blood cell hemolysis. The method comprises the following steps: acquiring a blood image set; the blood image set comprises a plurality of blood images and corresponding label information; using the blood image set to train a preset hemolysis detection model to obtain a trained hemolysis detection model; processing the acquired actual blood image by using the trained hemolysis detection model to obtain hemolysis detection result information; the hemolysis detection result information is used for representing erythrocyte hemolysis information of the acquired actual blood image. According to the method, the accuracy and the real-time performance of hemolysis detection of the model are improved, and the problems that the detection result is not objective enough and the accuracy is not high due to the fact that current suspension red blood cell hemolysis detection is often influenced by subjective factors of an operator are solved.

The invention relates to a human ghost protein mutant. The human ghost protein mutant is mainly characterized in that a beta subunit of the mutant has a mutation site named D470G compared with a wild type amino acid sequence. In addition, the invention also relates to a nucleic acid molecule of the human ghost protein mutant, a biological material and application thereof. The single base mutation of the ghost protein beta subunit gene provided by the invention can effectively inhibit complement activation mediated red blood cell hemolysis. The hematopoietic stem cells are extracted, single base mutation is performed on the ghost protein beta subunit gene of the hematopoietic stem cells, and then the hematopoietic stem cells are transplanted back to the body, so that the hematopoietic stem cells are expected to be used for treating red blood cell hemolysis mediated by complement activation and related diseases.

This invention discloses a method for preparing an aqueous extract of Artemisia argyi and its application, particularly in the preparation of drugs for treating allergic contact dermatitis. The preparation method includes step 1: naturally air-drying harvested Artemisia argyi leaves, followed by mechanical pulverization to obtain Artemisia floss raw material; step 2: mixing the Artemisia floss raw material with pure water, soaking at room temperature, extracting with ultrasound assistance, combining the extracts, decocting in a water bath, concentrating under reduced pressure, and freeze-drying to obtain the Artemisia argyi aqueous extract. This invention provides an Artemisia argyi aqueous extract, its preparation method, and its application. This Artemisia argyi aqueous extract has significant anti-allergic and anti-irritant effects, effectively inhibiting hyaluronidase activity, reducing erythrocyte hemolysis rate, and exhibiting good therapeutic effects on 2,4-dinitrofluorobenzene-induced allergic contact dermatitis. It also has high bioavailability and good safety. This invention lays an important foundation for developing efficient and safe natural anti-allergic products.

The invention discloses a nitrogen-containing spiro compound crystal form and a preparation method and application thereof. The invention provides a crystal form I of a nitrogen-containing spiro compound as shown in a formula A. The crystal form I of the nitrogen-containing spiro compound is radiated by Cu-K alpha, and an X-ray powder diffraction pattern expressed by a 2 theta angle has diffraction peaks at 5.67 + / -0.20 degrees, 11.37 + / -0.20 degrees, 16.69 + / -0.20 degrees, 17.32 + / -0.20 degrees and 19.73 + / -0.20 degrees. The invention provides a crystal form II of a nitrogen-containing spiro compound as shown in a formula A. The crystal form II is radiated by Cu-K alpha, and an X-ray powder diffraction pattern expressed by a 20 angle has diffraction peaks at the positions of 11.32 + / -0.20 degrees, 11.70 + / -0.20 degrees, 11.93 + / -0.20 degrees, 18.03 + / -0.20 degrees, 18.81 + / -0.20 degrees and 19.17 + / -0.20 degrees. The crystal form of the nitrogen-containing spiro compound has good stability and complement factor D inhibitory activity, has a good inhibitory effect on rabbit red blood cell hemolysis, and has high in-vivo exposure and high oral bioavailability.

The invention discloses a selenium-containing polymer as well as a preparation method and application thereof, and belongs to the technical field of medical polymer materials. The structure of the selenium-containing polymer is shown as a formula 1, a formula 2 or a formula 3. The selenium-containing polymer has excellent antibacterial and anti-inflammatory capabilities, oxidation resistance and blood compatibility, and has excellent antibacterial properties on gram-negative bacteria escherichia coli, pseudomonas aeruginosa, acinetobacter baumannii and gram-positive bacteria staphylococcus aureus; the compound has good scavenging capacity on DPPH free radicals based on electron transfer and ABTS free radicals based on hydrogen atom transfer, and red blood cells cannot be hemolyzed basically even under the high concentration (16 mg / mL). # imgabs0 #

The application discloses an image processing method and device for judging hemolysis of suspended red blood cells, and the method comprises the following steps: acquiring a blood image set; the blood image set comprises a plurality of blood images and corresponding label information; acquiring a blood parameter information set; the blood parameter information set comprises a plurality of blood parameters and corresponding label information; the blood parameters comprise hemoglobin concentration, reticulocyte concentration, indirect bilirubin concentration, lactate dehydrogenase concentration and globin concentration; a blood hemolysis composite detection model is constructed by using the blood image set and the blood parameter information set; the collected blood image and blood parameter are processed based on the blood hemolysis composite detection model, and hemolysis detection result information is obtained; the hemolysis detection result information is used for representing the red blood cell hemolysis information of the corresponding blood of the collected blood image and blood parameter.

The invention relates to a fluorescent probe based on a lanthanide series metal organic framework as well as a preparation method and application thereof, and belongs to the technical field of nano materials. The fluorescent probe provided by the invention is obtained by covalent crosslinking of a lanthanide series metal organic framework, a neutrophil elastin binding peptide and a P-selectin binding peptide, and the surface of the lanthanide series metal organic framework is modified with tetracyclic antibiotics and arginine. The fluorescent probe prepared by the invention specifically targets activated platelets and neutrophils, does not influence the cell survival rate, does not cause red blood cell hemolysis, and can be applied to preparation of thrombosis disease diagnosis products. The fluorescent probe is used for loading urokinase, stable and efficient delivery of urokinase is achieved, aggregation of platelets is inhibited, blood clot contraction is slowed down, and the fluorescent probe has a good thrombolysis effect and has the potential of being used for preparing drugs for treating thrombosis diseases. The required materials are easy to obtain, the preparation method is simple, and a new method is provided for diagnosis and treatment of thrombosis diseases.

The application discloses a peony endophyte, a method for producing exopolysaccharide by the peony endophyte and application of the exopolysaccharide, and the peony endophyte is obtained by culturing root, stem and leaf parts of peony, and the peony endophyte strain with high exopolysaccharide yield is screened out through color reaction and exopolysaccharide content determination; the strain is identified as Kosakonia cowanii LYH-L3, and the exopolysaccharide yield of the strain is 1.176 mg / mL. Safety determination experiments such as red blood cell hemolysis experiment and zebra fish acute toxicity experiment show that the exopolysaccharide of the application has no irritation and acute toxicity; and through zebra fish model experiment, in-vitro water absorption and water loss rate determination and human body test, it is shown that the exopolysaccharide produced by the peony endophyte strain has a moisturizing effect and a skin barrier repair function, and the application provides a theoretical basis for application of the exopolysaccharide produced by the peony endophyte in preparation of natural moisturizing and repairing cosmetics, skin care products, medicines and other related products, and provides a new target and a new direction for development and utilization of plant resources with Chinese characteristics.

The invention relates to the design of an antimicrobial peptide and its application in bacterial infection, which belongs to the field of biopharmaceutical polypeptide technology, and specifically relates to the design of an antimicrobial peptide through an amino acid replacement strategy and its application in the preparation of a drug for preventing or treating bacterial infection-related diseases. The polypeptide designed in the present invention has a short amino acid sequence, can be prepared efficiently and quickly, has strong antimicrobial activity, a broad antimicrobial spectrum, and weak hemolytic activity against red blood cells. It can be used for the prevention and treatment of diseases caused by various microbial infections, and has a good effect on the treatment of diseases such as skin trauma infection, sepsis, and pneumonia caused by bacterial infection.

This invention relates to an antimicrobial peptide derived from kitchen waste liquid and its preparation method, belonging to the field of biotechnology. Using kitchen waste liquid as raw material, this invention employs processes such as ultrasonic enzymatic hydrolysis, microbial fermentation, and purification to obtain peptides with antimicrobial activity. The antimicrobial peptides prepared by this invention exhibit broad-spectrum antimicrobial activity, inhibiting and killing various pathogens such as bacteria and fungi. Furthermore, the antimicrobial peptides show low hemolytic activity against erythrocytes, have short synthetic sequences, small molecular weights, and are easy to synthesize chemically. They can specifically kill pathogens in organisms while significantly reducing the cost of large-scale production. They can be used as peptide-based microbial inhibitors in fields such as feed, food, skincare products, pharmaceuticals, medical devices, animal husbandry, and agriculture.