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Peripheral blood lymphocyte micronucleus detection kit and detection method thereof

A lymphocyte and detection method technology, applied in the field of automatic in vitro diagnostic kits and its detection, can solve the problems of heavy workload and cumbersome steps, and achieve the effects of fast detection, guaranteed objectivity, and reduced differences

Inactive Publication Date: 2017-03-22
SHENZHEN PREVENTION & TREATMENT CENT FOR OCCUPATIONAL DISEASES
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

Routine peripheral blood lymphocyte micronucleus test, the steps are cumbersome, including peripheral blood sampling, inoculation, culture, film production, staining, microscopic examination and other steps, using manual film preparation, film reading and analysis, easily affected by the subjective factors, professional knowledge and experience of technicians impact, while the workload is heavy

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  • Peripheral blood lymphocyte micronucleus detection kit and detection method thereof
  • Peripheral blood lymphocyte micronucleus detection kit and detection method thereof
  • Peripheral blood lymphocyte micronucleus detection kit and detection method thereof

Examples

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Embodiment 1

[0054] Embodiment 1, a kind of detection kit and detection method thereof of peripheral blood lymphocyte micronucleus

[0055] 1. Detection kit and detection method for peripheral blood lymphocyte micronucleus

[0056] (1) Micronucleus Detection Kit of Peripheral Blood Lymphocytes

[0057] The detection kit of peripheral blood lymphocyte micronucleus of the present invention comprises: hemolysin, nucleic acid dye, membrane breaking reagent and positive control.

[0058] 1. Hemolysin

[0059] Dilute 10× hemolysin with deionized water into 1× working solution (1× hemolysin), and lyse according to the amount of 200 μl whole blood added to 1ml working solution (that is, the volume ratio of whole blood: 1× working solution is 1:5) Red blood cells cultured from whole blood.

[0060] 2. Nucleic acid dyes

[0061] In order to distinguish necrotic cells, apoptotic bodies, fragments and micronuclei, and eliminate false positive results, the present invention uses ethidium bromide mo...

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Abstract

The invention discloses a peripheral blood lymphocyte micronucleus detection kit and a detection method thereof. The detection kit provided by the invention comprises erythrocyte hemolysin, a red fluorescent dye solution, a lysis solution and a positive standard product, wherein the lysis solution comprises sodium chloride, sodium citrate, Triton X-114, green fluorescent dye and RNase; and a peripheral blood lymphocyte micronucleus detection method based on the kit and a flow cytometry detection method is provided. An experiment proves that the peripheral blood lymphocyte micronucleus detection kit and the peripheral blood lymphocyte micronucleus detection method are quick and accurate; on the premise of guaranteeing objective and true experimental results, the detection cost is reduced, and the experiment has repeatability and can be widely applied to clinical diagnosis and monitoring of occupational exposure genetic damage of healthy people, rehabilitation detection of genetic damage of cancer patient and cellular genetic science research.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to a detection kit for peripheral blood lymphocyte micronucleus and a detection method thereof, in particular to an automatic in vitro diagnostic kit for detecting peripheral blood lymphocyte micronucleus based on flow cytometry and its Detection method. Background technique [0002] The basic theory of micronucleus formation is that chromosome fragments or entire chromosomes are left in the cell fluid in the replicating cells due to chromosomal breakage or abnormal spindle function, which leads to mitotic chromosome distribution disorder. Currently, micronucleus tests include common culture micronucleus test (MNT) and cytokinesis block micronucleus test (CBMNT). [0003] Micronucleus test is an effective test method for genotoxicity test. Its detection end point is clear, counting is simple, and the results are reproducible. It can be widely used in different cell types and is easy to...

Claims

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Application Information

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IPC IPC(8): G01N15/14G01N1/28G01N1/30
CPCG01N15/14G01N1/28G01N1/30
Inventor 杨学琴黄先青张文
Owner SHENZHEN PREVENTION & TREATMENT CENT FOR OCCUPATIONAL DISEASES
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