The invention discloses toothpaste containing propolis, notoginseng roots, subshrubby peony root-barks, rhizome of deltoid goldthread and ural licorice roots and a preparation method of the toothpaste, and relates to the field of oral care. The toothpaste is natural care toothpaste containing propolis, notoginseng root, subshrubby peony root-bark, rhizome of deltoid goldthread and ural licorice root extracts, and has remarkable curative effects on gingivitis, gingival bleeding, oral ulcer, halitosis and the like. The propolis has the functions of promoting tissue repair, resisting and inhibiting bacteria, diminishing inflammation, alleviating pain and stopping bleeding. The notoginseng roots have the functions of promoting blood circulation, arresting bleeding, resisting thrombi, enhancingimmunity and effectively improving oral mucosa microcirculation. The subshrubby peony root-barks have the functions of clearing heat, cooling blood, diminishing inflammation, alleviating pain and promoting blood circulation to remove blood stasis. The rhizome of deltoid goldthread has the functions of clearing away the heart-fire, clearing away heat and toxic materials and resisting inflammationand viruses. The ural licorice roots have the functions of clearing away heat and toxic materials and discharging fire. Therefore, the toothpaste containing the propolis, notoginseng root, subshrubbypeony root-bark, rhizome of deltoid goldthread and ural licorice root extracts has more remarkable effects of relieving pain, diminishing inflammation, resisting gingivitis, gingival bleeding and pin,repairing oral mucosa tissues, inhibiting the bacteria and preventing halitosis.