Method for extracting antioxidation active substance from Cichorium endivia L.

A technology of antioxidant activity and extraction method, applied in the field of natural product extraction, can solve problems such as the extraction process of antioxidant substances of chrysanthemum, and achieve the effect of simple extraction and abundant plant resources.

Active Publication Date: 2010-10-27
BEIJING UNION GENIUS PHARMA TECH
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  • Method for extracting antioxidation active substance from Cichorium endivia L.
  • Method for extracting antioxidation active substance from Cichorium endivia L.
  • Method for extracting antioxidation active substance from Cichorium endivia L.

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[0013] 1 Preparation of Cultivated Chicory Extract (KJ-I)

[0014] The present invention is further described in detail through the following examples, but the technical content of this example is illustrative rather than limiting, and should not be used to limit the protection scope of the present invention.

[0015] The steps of the method for extracting antioxidant active substances from cultivated chicory involved in the present invention are as follows:

[0016] Cultivated chicory purchased from Beijing Xinfadi Vegetable Market, 5.8kg of dried whole herb was reflux extracted three times in 95% ethanol (80L×2h, 70L×1h, 68L×1h), filtered, combined filtrates, concentrated under reduced pressure, and the resulting infusion The cream was suspended in 70% ethanol solution (total volume 3.5L), extracted four times with petroleum ether (2L, 1.5L, 1.5L, 1.5L), the alcohol layer was concentrated under reduced pressure until it had no alcohol smell, and about 2.3L was added to suspe...

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Abstract

The invention relates to a method for extracting an antioxidation active substance from Cichorium endivia L., which adopts ethanol extraction, petroleum ether and ethyl acetate extraction for purification, macroporous absorption resin elution, concentration and drying to finally obtain the substance with high antioxidation activity and belongs to the field of natural product extraction. The Cichorium endivia L. extract has high radical-removing, antioxidation and reducing capabilities, the activity of the extract is higher than the activity of water-soluble vitamine E, and an erythrocyte hemolysis experiment indicates that the Cichorium endivia L. extract also has the antioxidation capability at the cell level. The method, which adopts Cichorium endivia L. as raw material to prepare the high-antioxidation active substance, has the advantages of simple technique and equipment and less investment, and is suitable for mass production and applicable to industries such as medicine and food additives, health-care products and cosmetics.

Description

technical field [0001] The invention belongs to the field of natural product extraction, in particular to a method for extracting antioxidant active substances from cultivated chicory. The extract has natural antioxidant activity and can be made into antioxidants to prevent food oxidation, prevent diseases, improve body immunity, and health products for prolonging life. technical background [0002] Free radicals and their closely related reactive oxygen species can cause lipid peroxidation and accelerate the oxidation process of lipids, thereby destroying the taste and quality of oil-rich products. In addition, they can also invade many important biomolecules in organisms, such as cell membranes, proteins and DNA, causing damage to cells and tissues. Therefore, preventing and removing free radicals and reactive oxygen species will be of great benefit to food preservation and human health, and the use of antioxidants is the most important means of prevention and treatment. ...

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Application Information

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IPC IPC(8): A23L3/3562A23L1/30A61K36/28A61K8/97A61P39/06A61Q19/00A23L33/105
Inventor 陈超杰秦海林王爱平魏金锋靳洪涛王英明
Owner BEIJING UNION GENIUS PHARMA TECH
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