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White needle mushroom JK01 strain and application thereof

A technology of white Flammulina velutipes and strains, applied in application, fungi, horticulture, etc., can solve the problems of long cultivation period, high requirements, poor resistance, etc., and achieve the effects of short cultivation period, improved production efficiency and thick stipe

Active Publication Date: 2017-10-03
BIOLOGY INST OF HEBEI ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The invention provides a white Flammulina velutipes JK01 strain and its application, which are used to solve the problems of current strains such as high equipment requirements, poor resistance, long cultivation period, many branches, and difficulty in splitting.

Method used

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  • White needle mushroom JK01 strain and application thereof
  • White needle mushroom JK01 strain and application thereof
  • White needle mushroom JK01 strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1 hybridization obtains JK01

[0032] The Flammulina velutipes strain JK01 was obtained by cross breeding.

[0033] Specific steps are as follows:

[0034] 1) Select the parent bacterial strain: select the bacterial strain Taichangbaiheza 19 as the parent, and the fruiting bodies of these two parents can be purchased in the market;

[0035] 2) Preparation of mother seed culture medium, liquid seed culture medium and cultivation medium: the formulations of each culture medium have been given, and will not be repeated here;

[0036] 3) Obtain 200 heterozygotes A by conventional cross-breeding methods;

[0037] 4) Cultivation and screening: The obtained heterozygotes were subjected to cultivation tests according to conventional methods, and through systematic screening, a target strain Jinza 9 was obtained from 200 heterozygotes A, which had fast hyphae growth, strong resistance, Early fruiting, high yield, light yellow color, thick stipe, not easy to open um...

Embodiment 2

[0040] Example 2 Strain Antagonism Experiment and ISSR Authenticity Analysis

[0041] Flammulina velutipes JK01 of the present invention and two parent strains Taichangbai and Za19 are test materials, and the culture medium used is PDA culture medium.

[0042] JK01 and Taichangbai, JK01 and Za19 were inoculated and cultured on the plate medium respectively, the inoculation points were 4 cm apart, and cultured at 25°C for 15 days. The results showed that the antagonistic lines of JK01 and Taichangbai, JK01 and Za19 were obvious at the junction of confrontation culture colonies, which indicated that JK01 was a different Flammulina velutipes strain from Taichangbai and Za19.

[0043] Authenticity Analysis of JK01 Strain ISSR Method

[0044]In order to prove the differences between the JK01 strain and the parental species Taichangbai and Za19, as well as the existing white Flammulina velutipes varieties F21 and Rijin, the strain authenticity analysis was carried out. According t...

Embodiment 3

[0048] Embodiment 3 disease resistance test

[0049] Trichoderma viride and pathogenic Pseudomonas aeruginosa (P.Aeruginosa) are the main diseases in the cultivation of Flammulina velutipes. The pathogenic bacteria Trichoderma viride and Pseudomonas aeruginosa isolated from Flammulina velutipes and Pseudomonas aeruginosa isolated and preserved in our unit were used as indicators to carry out confrontation culture. The disease resistance can be judged by observing the growth of the colony and the width of the antagonistic band. The narrower the antagonistic band, the stronger the disease resistance.

[0050] Activation of strains: Activation culture of Flammulina velutipes strain and Trichoderma virides strain in PDA medium at 25°C respectively, and the dish is ready for use after the dish is full. Before being used as bacteria species, bacteria cakes were prepared with a puncher with a diameter of 0.5 cm. Pseudomonas aeruginosa was activated in PDA medium at 25°C for 2 days,...

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Abstract

The invention belongs to the technical field of edible mushrooms, and particularly relates to a white needle mushroom JK01 strain and application thereof. A needle mushroom provided by the invention is named JK01, is obtained by hybridizing a Taichangbai strain and a hybridized strain 19 serving as parents through systemic breeding, belongs to needle mushroom (Flammulina velutipes), and is collected in the Institute of Microbiology, Chinese Academy of Sciences (address: No. 3, 1# Yard, Beichen West Road, Chaoyang District, Beijing) with a collection number CGMCCNo.13877 on June 14, 2017. Compared with the prior art, the white needle mushroom JK01 strain provided by the invention has the advantages of short culturing period, consistent growth, high stress resistance, high yield, suitability for industrial cultivation, thick stems, superior shape characteristic, crispy mouthfeel, contribution to splitting and cooking of products, high comprehensive performance, and wide market prospect.

Description

technical field [0001] The invention belongs to the technical field of edible fungi, and in particular relates to the JK01 strain of Flammulina velutipes and application thereof. Background technique [0002] Flammulina velutiper (Fr.) Sing is called at school, and it is not only a delicious food, but also a good health food. Flammulina velutipes is cold in nature, sweet and salty in taste, has the effects of nourishing the liver, benefiting the stomach, and anti-cancer. It is mainly used for liver diseases, gastrointestinal inflammation, ulcers, tumors and other diseases. Flammulina velutipes has a high zinc content, which is helpful for preventing male prostate diseases. Moreover, Flammulina velutipes is also a high-potassium and low-sodium food, which can prevent and treat high blood pressure and is beneficial to the elderly. The amino acid content of Flammulina velutipes is also very rich, which is higher than that of common mushrooms, especially the content of lysin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N15/04A01G1/04C12R1/645
CPCC12N15/04C12N1/145C12R2001/645
Inventor 张根伟刘振国李书生马宏刘昆昂尹淑丽
Owner BIOLOGY INST OF HEBEI ACAD OF SCI
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