The invention discloses a method for enriching X sperms of Nubia goats and an enrichment kit for X sperms of Nubia goats, and belongs to the technical field of
livestock breeding and
biology. The method for enriching the Nubia goat X sperms comprises the following steps: (1) carrying out
activity detection on Nubia goat sperms, and washing the Nubia goat sperms which are qualified in
activity detection to obtain washed
sperm samples; (2) adding a
sperm diluent containing R848 into the washed
sperm sample, mixing, and incubating at 37 DEG C for 45 minutes; and (3) after incubation is completed, collecting precipitated sperms at the bottom of the container, and washing with a sperm
diluent to remove R848 so as to obtain enriched Nubia goat X sperms. According to the method, efficient enrichment of the X sperms of the Nubia goats can be achieved through the steps of incubation,
sediment collection and washing, the activity of the X sperms of the Nubia goats obtained through enrichment is not affected, and the method has important value for artificial propagation of the Nubia goats.