The present invention provides a preparation method of a Nubia goat tripe cleaning agent. The method comprises the following steps: 25-30 parts of cortex mori, 15-20 parts of angelica sinensis, 15-20 parts of green tea, 12-16 parts of bamboo leaves, 6-10 parts of toona sinensis and 5-8 parts of dried bitter gourds are crushed into coarse powder, then the coarse powder is soaked into osmanthus fragrans flower wine for 12-24 h, the soaked coarse powder is sieved via a 80-100 mesh sieve and a filtrate is concentrated into an extractum; 15-20 parts of castor oil is added to a pot, the castor oil is heated, 4-7 parts of beeswax is added, the heating is stopped until the beeswax is completely melted, the mixture is cooled to a room temperature, the extractum is added, and the mixture is mixed evenly to prepare a material I; 35-40 parts of lotus leaves, 15-18 parts of citrus leaves, 12-15 parts of wheat bran, 8-12 parts of Chinese prickly ash leaves, 8-12 parts of cymbopogon citratus, 6-10 parts of bagasse and 4-6 parts of coffee residues are dried, the dried materials are crushed into fine powder, the fine powder is added into a pot to be stir-fried, the stir-fried fine powder is cooled, and the cooled fine powder is mixed evenly with radix puerariae starch to prepare a material II; 5-8 parts of brasenia schreberi and 5-8 parts of fresh gingers are cut into blocks, the blocks are added into a juice squeezer, water at an equal amount of the blocks is added to conduct a squeezing, and the filtrate is a material III; and the edible base is dissolved in the remaining water, then the material I, material II and material III are added, and the mixture is beat until a homogeneous slurry body is formed.