Tissue culture method for Japanese maple

A technique of tissue culture and Acer palmatum, applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve problems such as difficult rooting, difficult realization of factory production, and difficult survival of cuttings, and achieves low cost, pollution suppression, The effect of meeting the needs of large-scale industrial seedling cultivation and industrialization development

Active Publication Date: 2018-05-25
SICHUAN COLORLINK CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Acer palmatum ‘sango kaku edisburry’ (sango kaku edisburry) is a species of Acer palmatum that is difficult to root, and the cuttings are not easy to survive; due to the influence of the season and the scion of the female parent, it is difficult to realize its industrial production for grafting

Method used

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  • Tissue culture method for Japanese maple
  • Tissue culture method for Japanese maple
  • Tissue culture method for Japanese maple

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] A method for tissue culture of Acer palmatum, comprising:

[0034] Pretreatment: Take the current-year-old branches of Acer palmatum, cut them into budded stems with 1-2 buds, remove the leaves, sterilize with 75Vol% alcohol for 20-40s, wash with sterile water 1-3 times, Sterilize with 0.1wt% mercuric chloride for 3 to 8 minutes, wash with sterile water for 5 to 6 times, and obtain sterilized explants;

[0035] Start-up culture: inoculate the sterilized explants on the start-up medium for start-up culture to obtain axillary buds; the start-up medium includes: MS salts and H organic, supplemented with 0.1-0.3mg / L6-BA, 0.05 ~0.1mg / LNAA, 0.1~0.4%PPM;

[0036] Proliferation culture: inoculate the axillary buds in the proliferation medium for proliferation culture to obtain clustered buds; the proliferation medium includes: MS salts and H organic, supplemented with 0.01-0.1mg / L TDZ, 0.4-0.6mg / LCPPU;

[0037] Rooting culture: after removing the callus from the base of the c...

Embodiment 2

[0043] Effects of Components in Induction Medium on Germination of Axillary Buds

[0044] Pretreatment: Take the current-year-old branches of Acer palmatum, cut them into budded stems with 1-2 buds, remove the leaves, sterilize with 75Vol% alcohol for 20-40s, wash with sterile water 1-3 times, Sterilize with 0.1wt% mercuric chloride for 3 to 8 minutes, wash with sterile water for 5 to 6 times, and obtain sterilized explants;

[0045] Start-up culture: inoculate the sterilized explants on the start-up medium for start-up culture to obtain axillary buds with a length of 1-2 cm; the start-up medium includes: MS salts and H organic, supplemented with 6-BA, NAA, PPM, 20-30g / L sucrose, 4-6g / L agar. The pH value of the start-up medium is 5.6-5.8; the culture temperature of the start-up culture process is 24-26°C, the light intensity is 1500-2000Lx, and the light time is 12-14h / d.

[0046] Wherein, the Acer palmatum is selected from the cultivar Acer palmatum New Coral Pavilion, and...

Embodiment 3

[0053] Effects of Growth Hormone Concentration in Proliferation Medium on Proliferation of Axillary Buds

[0054] Pretreatment: Take the current-year-old branches of Acer palmatum, cut them into budded stems with 1-2 buds, remove the leaves, sterilize with 75Vol% alcohol for 20-40s, wash with sterile water 1-3 times, Sterilize with 0.1wt% mercuric chloride for 3 to 8 minutes, wash with sterile water for 5 to 6 times, and obtain sterilized explants;

[0055] Start-up culture: inoculate the sterilized explants in the start-up medium for start-up culture to obtain axillary buds with a length of 1-2 cm; the start-up medium includes: MS salts and H organic, supplemented with 0.2mg / L6 -BA, 0.05mg / LNAA, 0.2%PPM, 20~30g / L sucrose, 4~6g / L agar;

[0056] Proliferation culture: inoculate the axillary buds in the proliferation medium for proliferation culture to obtain clustered buds with a plant height of 2-3 cm; the proliferation medium includes: MS salts and H organic, supplemented wi...

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Abstract

The invention discloses a tissue culture method for Japanese maple. The tissue culture method for Japanese maple comprises the following steps: pretreatment: taking an annual shoot of the Japanese maple, shearing off a stem with a bud and a stem tip, and after removing redundant leaves, carrying out sterilizing and cleaning treatment to obtain a sterilized explant; initiation culture: inoculatingthe sterilized explant on an initiation culture medium and carrying out initiation culture to obtain stretched-out axillary buds; propagation culture: inoculating the axillary buds on a propagation culture medium and carrying out propagation culture to obtain cluster buds; and rooting culture: cutting off tissue culture from the bases of the cluster buds, then inoculating the cluster buds on a rooting culture medium, and carrying out rooting culture to obtain test-tube plantlets. The tissue culture and rapid propagation method is short in culture time, a culture process is simple and convenient, the breeding efficiency of a Japanese maple cultivated variety which is Acer palmatum 'sango kakuedisburry' (sango kaku edisburry) is improved, the survival rate of the induced axillary buds is 96%or above, the growth coefficient is 7-9, and the rooting rate is 92% or above. Requirements of large-scale industrialized seedling culture and industrialized development can be met.

Description

technical field [0001] The invention relates to the technical field of tissue culture, in particular to a method for tissue culture of Acer palmatum. Background technique [0002] Acer palmatum (scientific name: Acer palmatum Thunb.) is a small deciduous tree of the Aceraceae family; the crown is umbrella-shaped. The bark is smooth. Bark dark gray. Branchlets purple or lavender-green, old branches lavender-purple. Leaves are nearly round, base heart-shaped or nearly heart-shaped, palmate, often 7-parted, densely serrated. The back leaves bloom; the flowers are purple, polygamous, male and hermaphrodite; corymbs. Sepals ovate-lanceolate; petals elliptic or obovate. The young fruit is purple-red, brownish-yellow when ripe, with a spherical core, prominent veins, and two wings forming obtuse angles. The flowering and fruiting period is from May to September. [0003] Acer palmatum 'sango kaku edisburry' (sango kaku edisburry) is a cultivar of Acer palmatum, belonging to ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 蔡世林杨金财丁龙梅王洁
Owner SICHUAN COLORLINK CO LTD
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